Suppression of in vitro lymphocyte reactivity by cyclosporin A: existence of a population of drug-resistant cytotoxic lymphocytes View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

1980-08

AUTHORS

T. Horsburgh, P. Wood, L. Brent

ABSTRACT

Cyclosporin A (CS-A) is an unusual endecapeptide isolated from the fungi Cylindrocarpon lucidum Booth and Tri choderma polysporum1. It is a potent immunosuppressive drug that prevents rejection of kidney2–4 and heart5,6 allografts whilst having a low my elotoxicity7. Its mode of action is still unclear but its main target appears to be the T lymphocyte8. The mixed lymphocyte reaction (MLR) and cell-mediated lymphocytoxicity (CML) used here are generally regarded as in vitro correlates of allograft rejection9. Our data show that CS-A is a powerful inhibitor of MLR reactivity, regardless of whether the cells were obtained from normal or presensitized donors; that the CML of lymph node cells is likewise totally inhibited if the drug is added early during cell culture; and that, by contrast, the cytotoxic response of spleen cells from presensitized but not from normal mice is only partially inhibited, even with a tenfold increase in dose. It is therefore suggested that there exists a population of cytotoxic spleen cells that is relatively resistant to the action of this drug. More... »

PAGES

609-611

References to SciGraph publications

  • 1976-06. Cyclosporin A and C in APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
  • 1976-07. Biological effects of cyclosporin A: A new antilymphocytic agent in INFLAMMATION RESEARCH
  • Identifiers

    URI

    http://scigraph.springernature.com/pub.10.1038/286609a0

    DOI

    http://dx.doi.org/10.1038/286609a0

    DIMENSIONS

    https://app.dimensions.ai/details/publication/pub.1049738482

    PUBMED

    https://www.ncbi.nlm.nih.gov/pubmed/6447256


    Indexing Status Check whether this publication has been indexed by Scopus and Web Of Science using the SN Indexing Status Tool
    Incoming Citations Browse incoming citations for this publication using opencitations.net

    JSON-LD is the canonical representation for SciGraph data.

    TIP: You can open this SciGraph record using an external JSON-LD service: JSON-LD Playground Google SDTT

    [
      {
        "@context": "https://springernature.github.io/scigraph/jsonld/sgcontext.json", 
        "about": [
          {
            "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/11", 
            "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
            "name": "Medical and Health Sciences", 
            "type": "DefinedTerm"
          }, 
          {
            "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/1107", 
            "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
            "name": "Immunology", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Animals", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Cells, Cultured", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Cyclosporins", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Cytotoxicity, Immunologic", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Dose-Response Relationship, Drug", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Immune Tolerance", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Lymphocyte Activation", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Lymphocyte Culture Test, Mixed", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Mice", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Peptides, Cyclic", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "T-Lymphocytes", 
            "type": "DefinedTerm"
          }
        ], 
        "author": [
          {
            "affiliation": {
              "alternateName": "Department of Surgery, Leicester General Hospital, Leicester, UK", 
              "id": "http://www.grid.ac/institutes/grid.412934.9", 
              "name": [
                "Department of Immunology, St Mary's Hospital Medical School, W2 1PG, London, UK", 
                "Department of Surgery, Leicester General Hospital, Leicester, UK"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Horsburgh", 
            "givenName": "T.", 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "Department of Immunology, St Mary's Hospital Medical School, W2 1PG, London, UK", 
              "id": "http://www.grid.ac/institutes/grid.426467.5", 
              "name": [
                "Department of Immunology, St Mary's Hospital Medical School, W2 1PG, London, UK"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Wood", 
            "givenName": "P.", 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "Department of Immunology, St Mary's Hospital Medical School, W2 1PG, London, UK", 
              "id": "http://www.grid.ac/institutes/grid.426467.5", 
              "name": [
                "Department of Immunology, St Mary's Hospital Medical School, W2 1PG, London, UK"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Brent", 
            "givenName": "L.", 
            "id": "sg:person.01102552101.81", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01102552101.81"
            ], 
            "type": "Person"
          }
        ], 
        "citation": [
          {
            "id": "sg:pub.10.1007/bf01973261", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1051012626", 
              "https://doi.org/10.1007/bf01973261"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1007/bf00928431", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1025179406", 
              "https://doi.org/10.1007/bf00928431"
            ], 
            "type": "CreativeWork"
          }
        ], 
        "datePublished": "1980-08", 
        "datePublishedReg": "1980-08-01", 
        "description": "Cyclosporin A (CS-A) is an unusual endecapeptide isolated from the fungi Cylindrocarpon lucidum Booth and Tri choderma polysporum1. It is a potent immunosuppressive drug that prevents rejection of kidney2\u20134 and heart5,6 allografts whilst having a low my elotoxicity7. Its mode of action is still unclear but its main target appears to be the T lymphocyte8. The mixed lymphocyte reaction (MLR) and cell-mediated lymphocytoxicity (CML) used here are generally regarded as in vitro correlates of allograft rejection9. Our data show that CS-A is a powerful inhibitor of MLR reactivity, regardless of whether the cells were obtained from normal or presensitized donors; that the CML of lymph node cells is likewise totally inhibited if the drug is added early during cell culture; and that, by contrast, the cytotoxic response of spleen cells from presensitized but not from normal mice is only partially inhibited, even with a tenfold increase in dose. It is therefore suggested that there exists a population of cytotoxic spleen cells that is relatively resistant to the action of this drug.", 
        "genre": "article", 
        "id": "sg:pub.10.1038/286609a0", 
        "isAccessibleForFree": false, 
        "isPartOf": [
          {
            "id": "sg:journal.1018957", 
            "issn": [
              "0028-0836", 
              "1476-4687"
            ], 
            "name": "Nature", 
            "publisher": "Springer Nature", 
            "type": "Periodical"
          }, 
          {
            "issueNumber": "5773", 
            "type": "PublicationIssue"
          }, 
          {
            "type": "PublicationVolume", 
            "volumeNumber": "286"
          }
        ], 
        "keywords": [
          "mixed lymphocyte reaction", 
          "spleen cells", 
          "cytotoxic spleen cells", 
          "lymph node cells", 
          "potent immunosuppressive drug", 
          "presensitized donors", 
          "lymphocyte reactivity", 
          "immunosuppressive drugs", 
          "lymphocyte reaction", 
          "node cells", 
          "normal mice", 
          "cytotoxic lymphocytes", 
          "MLR reactivity", 
          "cytotoxic response", 
          "mode of action", 
          "drugs", 
          "cyclosporin", 
          "powerful inhibitor", 
          "cells", 
          "cell cultures", 
          "lymphocytoxicity", 
          "allografts", 
          "lymphocytes", 
          "population", 
          "endecapeptide", 
          "main target", 
          "mice", 
          "dose", 
          "tenfold increase", 
          "inhibitors", 
          "action", 
          "correlates", 
          "suppression", 
          "donors", 
          "reactivity", 
          "rejection", 
          "response", 
          "target", 
          "contrast", 
          "increase", 
          "culture", 
          "data", 
          "CS", 
          "reaction", 
          "booth", 
          "tri", 
          "existence", 
          "mode"
        ], 
        "name": "Suppression of in vitro lymphocyte reactivity by cyclosporin A: existence of a population of drug-resistant cytotoxic lymphocytes", 
        "pagination": "609-611", 
        "productId": [
          {
            "name": "dimensions_id", 
            "type": "PropertyValue", 
            "value": [
              "pub.1049738482"
            ]
          }, 
          {
            "name": "doi", 
            "type": "PropertyValue", 
            "value": [
              "10.1038/286609a0"
            ]
          }, 
          {
            "name": "pubmed_id", 
            "type": "PropertyValue", 
            "value": [
              "6447256"
            ]
          }
        ], 
        "sameAs": [
          "https://doi.org/10.1038/286609a0", 
          "https://app.dimensions.ai/details/publication/pub.1049738482"
        ], 
        "sdDataset": "articles", 
        "sdDatePublished": "2022-11-24T20:46", 
        "sdLicense": "https://scigraph.springernature.com/explorer/license/", 
        "sdPublisher": {
          "name": "Springer Nature - SN SciGraph project", 
          "type": "Organization"
        }, 
        "sdSource": "s3://com-springernature-scigraph/baseset/20221124/entities/gbq_results/article/article_176.jsonl", 
        "type": "ScholarlyArticle", 
        "url": "https://doi.org/10.1038/286609a0"
      }
    ]
     

    Download the RDF metadata as:  json-ld nt turtle xml License info

    HOW TO GET THIS DATA PROGRAMMATICALLY:

    JSON-LD is a popular format for linked data which is fully compatible with JSON.

    curl -H 'Accept: application/ld+json' 'https://scigraph.springernature.com/pub.10.1038/286609a0'

    N-Triples is a line-based linked data format ideal for batch operations.

    curl -H 'Accept: application/n-triples' 'https://scigraph.springernature.com/pub.10.1038/286609a0'

    Turtle is a human-readable linked data format.

    curl -H 'Accept: text/turtle' 'https://scigraph.springernature.com/pub.10.1038/286609a0'

    RDF/XML is a standard XML format for linked data.

    curl -H 'Accept: application/rdf+xml' 'https://scigraph.springernature.com/pub.10.1038/286609a0'


     

    This table displays all metadata directly associated to this object as RDF triples.

    177 TRIPLES      21 PREDICATES      87 URIs      77 LITERALS      18 BLANK NODES

    Subject Predicate Object
    1 sg:pub.10.1038/286609a0 schema:about N1ef6a40a7ee740ab98c9557309903ba5
    2 N2a651559ee1f4ae29a51f1a1bee05b71
    3 N4370b52ae52f46c982d301eca1495ff0
    4 N43a069e2c6594728bdac465284379462
    5 N45650643448644ca8e757b4c8544f344
    6 N570f661685c8492d9efc76c3ce553e1b
    7 N5c6407045228475c987cb6a16e82d205
    8 N88428f6a17a044b69c4343f61c2c95ee
    9 N9469a59d130d4b8189bf874e82900626
    10 Na2159b8fa8cb44ed9637aaa4a573ff42
    11 Ne5a91fcb9fda46d0b7be21a4edd1927c
    12 anzsrc-for:11
    13 anzsrc-for:1107
    14 schema:author N2b65a1f393024af7a0f21e5679305f22
    15 schema:citation sg:pub.10.1007/bf00928431
    16 sg:pub.10.1007/bf01973261
    17 schema:datePublished 1980-08
    18 schema:datePublishedReg 1980-08-01
    19 schema:description Cyclosporin A (CS-A) is an unusual endecapeptide isolated from the fungi Cylindrocarpon lucidum Booth and Tri choderma polysporum1. It is a potent immunosuppressive drug that prevents rejection of kidney2–4 and heart5,6 allografts whilst having a low my elotoxicity7. Its mode of action is still unclear but its main target appears to be the T lymphocyte8. The mixed lymphocyte reaction (MLR) and cell-mediated lymphocytoxicity (CML) used here are generally regarded as in vitro correlates of allograft rejection9. Our data show that CS-A is a powerful inhibitor of MLR reactivity, regardless of whether the cells were obtained from normal or presensitized donors; that the CML of lymph node cells is likewise totally inhibited if the drug is added early during cell culture; and that, by contrast, the cytotoxic response of spleen cells from presensitized but not from normal mice is only partially inhibited, even with a tenfold increase in dose. It is therefore suggested that there exists a population of cytotoxic spleen cells that is relatively resistant to the action of this drug.
    20 schema:genre article
    21 schema:isAccessibleForFree false
    22 schema:isPartOf N225a27e1e4ac4611be4af1608e89282a
    23 N92c0a05840844bd585348b9e4168a7d1
    24 sg:journal.1018957
    25 schema:keywords CS
    26 MLR reactivity
    27 action
    28 allografts
    29 booth
    30 cell cultures
    31 cells
    32 contrast
    33 correlates
    34 culture
    35 cyclosporin
    36 cytotoxic lymphocytes
    37 cytotoxic response
    38 cytotoxic spleen cells
    39 data
    40 donors
    41 dose
    42 drugs
    43 endecapeptide
    44 existence
    45 immunosuppressive drugs
    46 increase
    47 inhibitors
    48 lymph node cells
    49 lymphocyte reaction
    50 lymphocyte reactivity
    51 lymphocytes
    52 lymphocytoxicity
    53 main target
    54 mice
    55 mixed lymphocyte reaction
    56 mode
    57 mode of action
    58 node cells
    59 normal mice
    60 population
    61 potent immunosuppressive drug
    62 powerful inhibitor
    63 presensitized donors
    64 reaction
    65 reactivity
    66 rejection
    67 response
    68 spleen cells
    69 suppression
    70 target
    71 tenfold increase
    72 tri
    73 schema:name Suppression of in vitro lymphocyte reactivity by cyclosporin A: existence of a population of drug-resistant cytotoxic lymphocytes
    74 schema:pagination 609-611
    75 schema:productId N145087d94990425bad1638d7005256c3
    76 N6eb45df98c33401d891469b0c343def6
    77 N74bdcd27dbb145da9b0f8b0703703696
    78 schema:sameAs https://app.dimensions.ai/details/publication/pub.1049738482
    79 https://doi.org/10.1038/286609a0
    80 schema:sdDatePublished 2022-11-24T20:46
    81 schema:sdLicense https://scigraph.springernature.com/explorer/license/
    82 schema:sdPublisher Ndac1f1964c804e688496b7854e4b076d
    83 schema:url https://doi.org/10.1038/286609a0
    84 sgo:license sg:explorer/license/
    85 sgo:sdDataset articles
    86 rdf:type schema:ScholarlyArticle
    87 N145087d94990425bad1638d7005256c3 schema:name doi
    88 schema:value 10.1038/286609a0
    89 rdf:type schema:PropertyValue
    90 N15447c8da105459397f144cec302076f schema:affiliation grid-institutes:grid.412934.9
    91 schema:familyName Horsburgh
    92 schema:givenName T.
    93 rdf:type schema:Person
    94 N1ef6a40a7ee740ab98c9557309903ba5 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    95 schema:name Cytotoxicity, Immunologic
    96 rdf:type schema:DefinedTerm
    97 N225a27e1e4ac4611be4af1608e89282a schema:volumeNumber 286
    98 rdf:type schema:PublicationVolume
    99 N2a651559ee1f4ae29a51f1a1bee05b71 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    100 schema:name Lymphocyte Culture Test, Mixed
    101 rdf:type schema:DefinedTerm
    102 N2b65a1f393024af7a0f21e5679305f22 rdf:first N15447c8da105459397f144cec302076f
    103 rdf:rest N4d5435f021be4857818306e21f38e7f5
    104 N4370b52ae52f46c982d301eca1495ff0 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    105 schema:name Cells, Cultured
    106 rdf:type schema:DefinedTerm
    107 N43a069e2c6594728bdac465284379462 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    108 schema:name Immune Tolerance
    109 rdf:type schema:DefinedTerm
    110 N45650643448644ca8e757b4c8544f344 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    111 schema:name Cyclosporins
    112 rdf:type schema:DefinedTerm
    113 N4d5435f021be4857818306e21f38e7f5 rdf:first N64d577ac78f441d99b459b7b498c7f4d
    114 rdf:rest Nddd86c3b3aa949f7a4c542583c74b92a
    115 N570f661685c8492d9efc76c3ce553e1b schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    116 schema:name Lymphocyte Activation
    117 rdf:type schema:DefinedTerm
    118 N5c6407045228475c987cb6a16e82d205 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    119 schema:name Dose-Response Relationship, Drug
    120 rdf:type schema:DefinedTerm
    121 N64d577ac78f441d99b459b7b498c7f4d schema:affiliation grid-institutes:grid.426467.5
    122 schema:familyName Wood
    123 schema:givenName P.
    124 rdf:type schema:Person
    125 N6eb45df98c33401d891469b0c343def6 schema:name pubmed_id
    126 schema:value 6447256
    127 rdf:type schema:PropertyValue
    128 N74bdcd27dbb145da9b0f8b0703703696 schema:name dimensions_id
    129 schema:value pub.1049738482
    130 rdf:type schema:PropertyValue
    131 N88428f6a17a044b69c4343f61c2c95ee schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    132 schema:name T-Lymphocytes
    133 rdf:type schema:DefinedTerm
    134 N92c0a05840844bd585348b9e4168a7d1 schema:issueNumber 5773
    135 rdf:type schema:PublicationIssue
    136 N9469a59d130d4b8189bf874e82900626 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    137 schema:name Peptides, Cyclic
    138 rdf:type schema:DefinedTerm
    139 Na2159b8fa8cb44ed9637aaa4a573ff42 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    140 schema:name Mice
    141 rdf:type schema:DefinedTerm
    142 Ndac1f1964c804e688496b7854e4b076d schema:name Springer Nature - SN SciGraph project
    143 rdf:type schema:Organization
    144 Nddd86c3b3aa949f7a4c542583c74b92a rdf:first sg:person.01102552101.81
    145 rdf:rest rdf:nil
    146 Ne5a91fcb9fda46d0b7be21a4edd1927c schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    147 schema:name Animals
    148 rdf:type schema:DefinedTerm
    149 anzsrc-for:11 schema:inDefinedTermSet anzsrc-for:
    150 schema:name Medical and Health Sciences
    151 rdf:type schema:DefinedTerm
    152 anzsrc-for:1107 schema:inDefinedTermSet anzsrc-for:
    153 schema:name Immunology
    154 rdf:type schema:DefinedTerm
    155 sg:journal.1018957 schema:issn 0028-0836
    156 1476-4687
    157 schema:name Nature
    158 schema:publisher Springer Nature
    159 rdf:type schema:Periodical
    160 sg:person.01102552101.81 schema:affiliation grid-institutes:grid.426467.5
    161 schema:familyName Brent
    162 schema:givenName L.
    163 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01102552101.81
    164 rdf:type schema:Person
    165 sg:pub.10.1007/bf00928431 schema:sameAs https://app.dimensions.ai/details/publication/pub.1025179406
    166 https://doi.org/10.1007/bf00928431
    167 rdf:type schema:CreativeWork
    168 sg:pub.10.1007/bf01973261 schema:sameAs https://app.dimensions.ai/details/publication/pub.1051012626
    169 https://doi.org/10.1007/bf01973261
    170 rdf:type schema:CreativeWork
    171 grid-institutes:grid.412934.9 schema:alternateName Department of Surgery, Leicester General Hospital, Leicester, UK
    172 schema:name Department of Immunology, St Mary's Hospital Medical School, W2 1PG, London, UK
    173 Department of Surgery, Leicester General Hospital, Leicester, UK
    174 rdf:type schema:Organization
    175 grid-institutes:grid.426467.5 schema:alternateName Department of Immunology, St Mary's Hospital Medical School, W2 1PG, London, UK
    176 schema:name Department of Immunology, St Mary's Hospital Medical School, W2 1PG, London, UK
    177 rdf:type schema:Organization
     




    Preview window. Press ESC to close (or click here)


    ...