Isolation of the chicken thymidine kinase gene by plasmid rescue View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

1980-05

AUTHORS

Manuel Perucho, Douglas Hanahan, Leah Lipsich, Michael Wigler

ABSTRACT

We have used the bacterial plasmid pBR322 as a vehicle to isolate genes coding for selectable markers from higher eukaryotes. In this way, we have obtained the chicken thymidine kinase (tk) gene as a 2.2-kilobase EcoRI/HindIII insert in pBR322. The cloned gene transforms tk− animal cells with an efficiency equal to that of the cloned herpes simplex virus-1 tk gene. More... »

PAGES

207-210

Identifiers

URI

http://scigraph.springernature.com/pub.10.1038/285207a0

DOI

http://dx.doi.org/10.1038/285207a0

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1009045089

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/6246445


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