Nucleotide sequence of the hepatitis B virus genome (subtype ayw) cloned in E. coli View Full Text


Ontology type: schema:ScholarlyArticle      Open Access: True


Article Info

DATE

1979-10

AUTHORS

Francis Galibert, Elisabeth Mandart, Françoise Fitoussi, Pierre Tiollais, Patrick Charnay

ABSTRACT

The complete nucleotide sequence of hepatitis B virus genome (subtype ayw) cloned in Escherichia coli has been determined using the Maxam and Gilbert method and the dideoxynucleotide method. This sequence is 3,182 nucleotides long. Location of the nonsense codons shows that the coding capacity of the L chain is larger than the coding capacity of the Schain. Eight open regions, able to code for polypeptide chains larger than 100 amino acids, have been located. Region 6, which is the largest, covers more than 80% of the genome. The gene S which codes for polypeptide I of the Hbs Ag and was previously located between coordinates 95.1 and 73.6 is contained in region 7. More... »

PAGES

646-650

Journal

TITLE

Nature

ISSUE

5733

VOLUME

281

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  • Identifiers

    URI

    http://scigraph.springernature.com/pub.10.1038/281646a0

    DOI

    http://dx.doi.org/10.1038/281646a0

    DIMENSIONS

    https://app.dimensions.ai/details/publication/pub.1004769607

    PUBMED

    https://www.ncbi.nlm.nih.gov/pubmed/399327


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