Direct expression in Escherichia coli of a DNA sequence coding for human growth hormone View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

1979-10

AUTHORS

David V. Goeddel, Herbert L. Heyneker, Toyohara Hozumi, Rene Arentzen, Keiichi Itakura, Daniel G. Yansura, Michael J. Ross, Giuseppe Miozzari, Roberto Crea, Peter H. Seeburg

ABSTRACT

DNA coding for human growth hormone was constructed by using chemically synthesised DNA in conjunction with enzymatically prepared cDNA. This ‘hybrid’ gene was expressed in Escherichia coli under the control of the lac promoter. A polypeptide was produced having the size and immunological properties characteristic of mature human growth hormone.

PAGES

544-548

Journal

TITLE

Nature

ISSUE

5732

VOLUME

281

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  • Identifiers

    URI

    http://scigraph.springernature.com/pub.10.1038/281544a0

    DOI

    http://dx.doi.org/10.1038/281544a0

    DIMENSIONS

    https://app.dimensions.ai/details/publication/pub.1039289365

    PUBMED

    https://www.ncbi.nlm.nih.gov/pubmed/386136


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