Properties of haematopoietic stem cells surviving 5-fluorouracil treatment: evidence for a pre-CFU-S cell? View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

1979-10

AUTHORS

G S Hodgson, T R Bradley

ABSTRACT

The spleen colony assay of Till and McCulloch1, which is a clonal assay2, is considered to be a measure of the stem cell content of haematopoietic tissue. Stem cells are a heterogeneous population3–6 with respect to their capacity to generate further stem cells (assayed as colony-forming units in spleen, CFU-S) and progenitors of red cells, granulocytes, macrophages and platelets. It has been suggested that stem cells are organised for use according to their generation age5,6. There is evidence to indicate that drugs such as hydroxyurea (HU) and 5-fluorouracil (5-FU), which preferentially eliminate proliferating cells, spare non-cycling stem cells which have a high capacity to generate other stem cells6 and progenitors of granulocytes and macrophages6 as well as megakaryocytes7. We show here, however, that the CFU-S content of haematopoietic tissue is not an adequate predictor of the capacity of that tissue to repopulate bone marrow of a lethally irradiated host. Furthermore it appears that haematopoietic stem cells are not only heterogeneous with respect to their capacity to generate other stem cells and progenitors of haemic cells in the spleen but also differ in their capacity to ‘home’ to either the bone marrow or spleen. The evidence suggests a class of ‘primitive’ haematopoietic stem cell (pre-CFU-S) that accumulates in the bone marrow to give rise to cells capable of growing in spleen. More... »

PAGES

381-382

Identifiers

URI

http://scigraph.springernature.com/pub.10.1038/281381a0

DOI

http://dx.doi.org/10.1038/281381a0

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1013220393

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/481601


Indexing Status Check whether this publication has been indexed by Scopus and Web Of Science using the SN Indexing Status Tool
Incoming Citations Browse incoming citations for this publication using opencitations.net

JSON-LD is the canonical representation for SciGraph data.

TIP: You can open this SciGraph record using an external JSON-LD service: JSON-LD Playground Google SDTT

[
  {
    "@context": "https://springernature.github.io/scigraph/jsonld/sgcontext.json", 
    "about": [
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/1102", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Cardiorespiratory Medicine and Haematology", 
        "type": "DefinedTerm"
      }, 
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/11", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Medical and Health Sciences", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Animals", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Bone Marrow", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Bone Marrow Cells", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Cell Survival", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Fluorouracil", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Hematopoietic Stem Cells", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Mice", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Spleen", 
        "type": "DefinedTerm"
      }
    ], 
    "author": [
      {
        "familyName": "Hodgson", 
        "givenName": "G S", 
        "type": "Person"
      }, 
      {
        "familyName": "Bradley", 
        "givenName": "T R", 
        "type": "Person"
      }
    ], 
    "citation": [
      {
        "id": "https://doi.org/10.1111/j.1365-2184.1979.tb00110.x", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1020466327"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1002/jcp.1040970327", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1045285177"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/197452a0", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1050179231", 
          "https://doi.org/10.1038/197452a0"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/264068a0", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1051975381", 
          "https://doi.org/10.1038/264068a0"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/264068a0", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1051975381", 
          "https://doi.org/10.1038/264068a0"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.2307/3570892", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1070374292"
        ], 
        "type": "CreativeWork"
      }
    ], 
    "datePublished": "1979-10", 
    "datePublishedReg": "1979-10-01", 
    "description": "The spleen colony assay of Till and McCulloch1, which is a clonal assay2, is considered to be a measure of the stem cell content of haematopoietic tissue. Stem cells are a heterogeneous population3\u20136 with respect to their capacity to generate further stem cells (assayed as colony-forming units in spleen, CFU-S) and progenitors of red cells, granulocytes, macrophages and platelets. It has been suggested that stem cells are organised for use according to their generation age5,6. There is evidence to indicate that drugs such as hydroxyurea (HU) and 5-fluorouracil (5-FU), which preferentially eliminate proliferating cells, spare non-cycling stem cells which have a high capacity to generate other stem cells6 and progenitors of granulocytes and macrophages6 as well as megakaryocytes7. We show here, however, that the CFU-S content of haematopoietic tissue is not an adequate predictor of the capacity of that tissue to repopulate bone marrow of a lethally irradiated host. Furthermore it appears that haematopoietic stem cells are not only heterogeneous with respect to their capacity to generate other stem cells and progenitors of haemic cells in the spleen but also differ in their capacity to \u2018home\u2019 to either the bone marrow or spleen. The evidence suggests a class of \u2018primitive\u2019 haematopoietic stem cell (pre-CFU-S) that accumulates in the bone marrow to give rise to cells capable of growing in spleen.", 
    "genre": "research_article", 
    "id": "sg:pub.10.1038/281381a0", 
    "inLanguage": [
      "en"
    ], 
    "isAccessibleForFree": false, 
    "isPartOf": [
      {
        "id": "sg:journal.1018957", 
        "issn": [
          "0090-0028", 
          "1476-4687"
        ], 
        "name": "Nature", 
        "type": "Periodical"
      }, 
      {
        "issueNumber": "5730", 
        "type": "PublicationIssue"
      }, 
      {
        "type": "PublicationVolume", 
        "volumeNumber": "281"
      }
    ], 
    "name": "Properties of haematopoietic stem cells surviving 5-fluorouracil treatment: evidence for a pre-CFU-S cell?", 
    "pagination": "381-382", 
    "productId": [
      {
        "name": "readcube_id", 
        "type": "PropertyValue", 
        "value": [
          "3efb034f002206848429f7224fffed9038800b44567466db73713096fbdfcecb"
        ]
      }, 
      {
        "name": "pubmed_id", 
        "type": "PropertyValue", 
        "value": [
          "481601"
        ]
      }, 
      {
        "name": "nlm_unique_id", 
        "type": "PropertyValue", 
        "value": [
          "0410462"
        ]
      }, 
      {
        "name": "doi", 
        "type": "PropertyValue", 
        "value": [
          "10.1038/281381a0"
        ]
      }, 
      {
        "name": "dimensions_id", 
        "type": "PropertyValue", 
        "value": [
          "pub.1013220393"
        ]
      }
    ], 
    "sameAs": [
      "https://doi.org/10.1038/281381a0", 
      "https://app.dimensions.ai/details/publication/pub.1013220393"
    ], 
    "sdDataset": "articles", 
    "sdDatePublished": "2019-04-11T12:27", 
    "sdLicense": "https://scigraph.springernature.com/explorer/license/", 
    "sdPublisher": {
      "name": "Springer Nature - SN SciGraph project", 
      "type": "Organization"
    }, 
    "sdSource": "s3://com-uberresearch-data-dimensions-target-20181106-alternative/cleanup/v134/2549eaecd7973599484d7c17b260dba0a4ecb94b/merge/v9/a6c9fde33151104705d4d7ff012ea9563521a3ce/jats-lookup/v90/0000000362_0000000362/records_87119_00000000.jsonl", 
    "type": "ScholarlyArticle", 
    "url": "http://www.nature.com/articles/281381a0"
  }
]
 

Download the RDF metadata as:  json-ld nt turtle xml License info

HOW TO GET THIS DATA PROGRAMMATICALLY:

JSON-LD is a popular format for linked data which is fully compatible with JSON.

curl -H 'Accept: application/ld+json' 'https://scigraph.springernature.com/pub.10.1038/281381a0'

N-Triples is a line-based linked data format ideal for batch operations.

curl -H 'Accept: application/n-triples' 'https://scigraph.springernature.com/pub.10.1038/281381a0'

Turtle is a human-readable linked data format.

curl -H 'Accept: text/turtle' 'https://scigraph.springernature.com/pub.10.1038/281381a0'

RDF/XML is a standard XML format for linked data.

curl -H 'Accept: application/rdf+xml' 'https://scigraph.springernature.com/pub.10.1038/281381a0'


 

This table displays all metadata directly associated to this object as RDF triples.

118 TRIPLES      21 PREDICATES      42 URIs      29 LITERALS      17 BLANK NODES

Subject Predicate Object
1 sg:pub.10.1038/281381a0 schema:about N030937af66e64ed1aff4d7e4672f54da
2 N136a9018698d459497b871bbebc9b2f8
3 N20b1f70200c747b3996c0890b5be6a8d
4 N44d5a42c8adb4daaa17ace839ef95f29
5 N6ff7c3a7c3214a6a9c93595b01a0811a
6 N86d772cc21424e42be37676f912b93d5
7 Ne57e89a900bd4f46bbb3013155104d0b
8 Nfb8ac113db244aa0bc46e80ae78a6046
9 anzsrc-for:11
10 anzsrc-for:1102
11 schema:author N23fe2b29ff9945729068de66833648ae
12 schema:citation sg:pub.10.1038/197452a0
13 sg:pub.10.1038/264068a0
14 https://doi.org/10.1002/jcp.1040970327
15 https://doi.org/10.1111/j.1365-2184.1979.tb00110.x
16 https://doi.org/10.2307/3570892
17 schema:datePublished 1979-10
18 schema:datePublishedReg 1979-10-01
19 schema:description The spleen colony assay of Till and McCulloch1, which is a clonal assay2, is considered to be a measure of the stem cell content of haematopoietic tissue. Stem cells are a heterogeneous population3–6 with respect to their capacity to generate further stem cells (assayed as colony-forming units in spleen, CFU-S) and progenitors of red cells, granulocytes, macrophages and platelets. It has been suggested that stem cells are organised for use according to their generation age5,6. There is evidence to indicate that drugs such as hydroxyurea (HU) and 5-fluorouracil (5-FU), which preferentially eliminate proliferating cells, spare non-cycling stem cells which have a high capacity to generate other stem cells6 and progenitors of granulocytes and macrophages6 as well as megakaryocytes7. We show here, however, that the CFU-S content of haematopoietic tissue is not an adequate predictor of the capacity of that tissue to repopulate bone marrow of a lethally irradiated host. Furthermore it appears that haematopoietic stem cells are not only heterogeneous with respect to their capacity to generate other stem cells and progenitors of haemic cells in the spleen but also differ in their capacity to ‘home’ to either the bone marrow or spleen. The evidence suggests a class of ‘primitive’ haematopoietic stem cell (pre-CFU-S) that accumulates in the bone marrow to give rise to cells capable of growing in spleen.
20 schema:genre research_article
21 schema:inLanguage en
22 schema:isAccessibleForFree false
23 schema:isPartOf N8798f13765b643c5be4bfa0a80d09249
24 Nc8310b1f802c47c190230967dfa3067f
25 sg:journal.1018957
26 schema:name Properties of haematopoietic stem cells surviving 5-fluorouracil treatment: evidence for a pre-CFU-S cell?
27 schema:pagination 381-382
28 schema:productId N2245181023e74dcd8df6c7cee1ef6f31
29 N2d82f6549f454f5ab3135ccd11808ad2
30 N3f3c426aae944543b54bf50ffd2cf7c3
31 N82f6e31da1e9498892266861fbfd089c
32 Nbce44d5294a542aea245431cc66ca401
33 schema:sameAs https://app.dimensions.ai/details/publication/pub.1013220393
34 https://doi.org/10.1038/281381a0
35 schema:sdDatePublished 2019-04-11T12:27
36 schema:sdLicense https://scigraph.springernature.com/explorer/license/
37 schema:sdPublisher Nbc919200662e481084fe37ad0febba25
38 schema:url http://www.nature.com/articles/281381a0
39 sgo:license sg:explorer/license/
40 sgo:sdDataset articles
41 rdf:type schema:ScholarlyArticle
42 N030937af66e64ed1aff4d7e4672f54da schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
43 schema:name Animals
44 rdf:type schema:DefinedTerm
45 N0bdaf00d80c1468391c8ed8e36b850a6 schema:familyName Hodgson
46 schema:givenName G S
47 rdf:type schema:Person
48 N136a9018698d459497b871bbebc9b2f8 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
49 schema:name Cell Survival
50 rdf:type schema:DefinedTerm
51 N20b1f70200c747b3996c0890b5be6a8d schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
52 schema:name Mice
53 rdf:type schema:DefinedTerm
54 N2245181023e74dcd8df6c7cee1ef6f31 schema:name readcube_id
55 schema:value 3efb034f002206848429f7224fffed9038800b44567466db73713096fbdfcecb
56 rdf:type schema:PropertyValue
57 N23fe2b29ff9945729068de66833648ae rdf:first N0bdaf00d80c1468391c8ed8e36b850a6
58 rdf:rest N6682adfac18b467e982e2d6c22e4c4ca
59 N277d08021bd1410b9c0c47fa1ba3d702 schema:familyName Bradley
60 schema:givenName T R
61 rdf:type schema:Person
62 N2d82f6549f454f5ab3135ccd11808ad2 schema:name nlm_unique_id
63 schema:value 0410462
64 rdf:type schema:PropertyValue
65 N3f3c426aae944543b54bf50ffd2cf7c3 schema:name dimensions_id
66 schema:value pub.1013220393
67 rdf:type schema:PropertyValue
68 N44d5a42c8adb4daaa17ace839ef95f29 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
69 schema:name Spleen
70 rdf:type schema:DefinedTerm
71 N6682adfac18b467e982e2d6c22e4c4ca rdf:first N277d08021bd1410b9c0c47fa1ba3d702
72 rdf:rest rdf:nil
73 N6ff7c3a7c3214a6a9c93595b01a0811a schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
74 schema:name Hematopoietic Stem Cells
75 rdf:type schema:DefinedTerm
76 N82f6e31da1e9498892266861fbfd089c schema:name pubmed_id
77 schema:value 481601
78 rdf:type schema:PropertyValue
79 N86d772cc21424e42be37676f912b93d5 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
80 schema:name Bone Marrow Cells
81 rdf:type schema:DefinedTerm
82 N8798f13765b643c5be4bfa0a80d09249 schema:issueNumber 5730
83 rdf:type schema:PublicationIssue
84 Nbc919200662e481084fe37ad0febba25 schema:name Springer Nature - SN SciGraph project
85 rdf:type schema:Organization
86 Nbce44d5294a542aea245431cc66ca401 schema:name doi
87 schema:value 10.1038/281381a0
88 rdf:type schema:PropertyValue
89 Nc8310b1f802c47c190230967dfa3067f schema:volumeNumber 281
90 rdf:type schema:PublicationVolume
91 Ne57e89a900bd4f46bbb3013155104d0b schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
92 schema:name Fluorouracil
93 rdf:type schema:DefinedTerm
94 Nfb8ac113db244aa0bc46e80ae78a6046 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
95 schema:name Bone Marrow
96 rdf:type schema:DefinedTerm
97 anzsrc-for:11 schema:inDefinedTermSet anzsrc-for:
98 schema:name Medical and Health Sciences
99 rdf:type schema:DefinedTerm
100 anzsrc-for:1102 schema:inDefinedTermSet anzsrc-for:
101 schema:name Cardiorespiratory Medicine and Haematology
102 rdf:type schema:DefinedTerm
103 sg:journal.1018957 schema:issn 0090-0028
104 1476-4687
105 schema:name Nature
106 rdf:type schema:Periodical
107 sg:pub.10.1038/197452a0 schema:sameAs https://app.dimensions.ai/details/publication/pub.1050179231
108 https://doi.org/10.1038/197452a0
109 rdf:type schema:CreativeWork
110 sg:pub.10.1038/264068a0 schema:sameAs https://app.dimensions.ai/details/publication/pub.1051975381
111 https://doi.org/10.1038/264068a0
112 rdf:type schema:CreativeWork
113 https://doi.org/10.1002/jcp.1040970327 schema:sameAs https://app.dimensions.ai/details/publication/pub.1045285177
114 rdf:type schema:CreativeWork
115 https://doi.org/10.1111/j.1365-2184.1979.tb00110.x schema:sameAs https://app.dimensions.ai/details/publication/pub.1020466327
116 rdf:type schema:CreativeWork
117 https://doi.org/10.2307/3570892 schema:sameAs https://app.dimensions.ai/details/publication/pub.1070374292
118 rdf:type schema:CreativeWork
 




Preview window. Press ESC to close (or click here)


...