Monoclonal antibody to a human histocompatibility alloantigen, HLA-A2 View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

1978-11

AUTHORS

P. PARHAM, W. F. BODMER

ABSTRACT

ANTIGENS of the HLA-A, -B, -C and -DR series have been defined mainly using sera from multiparous women1, which generally are comparatively weak, serologically complex and available in only limited quantities. Furthermore, large screening programmes are required to find usable sera, especially for rare specificities. Although antisera to HLA determinants can be produced in high titres by immunising animals of various species with purified HLA-A, -B, -C, -D antigens2–5, they usually require extensive absorption to reveal polymorphic specificity and so have not so far been used as routine tissue-typing reagents. The development of techniques for producing hybrid cell lines secreting monoclonal antibodies of defined specificity6,7, provides a way of circumventing the problems of generating human polymorphic antisera by heteroimmunisation and has already been used to produce a monoclonal antibody against the human blood group A determinant8. Here we describe the production of a cell line secreting an anti-HLA-A2 antibody. More... »

PAGES

397

Identifiers

URI

http://scigraph.springernature.com/pub.10.1038/276397a0

DOI

http://dx.doi.org/10.1038/276397a0

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1027451765

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/714164


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