Uptake of charged lipid vesicles by isolated tomato protoplasts View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

1978-10

AUTHORS

A. C. CASSELLS

ABSTRACT

LIPID vesicles act as carriers of biologically active materials into animal cells1–3. A similar role might be envisaged for vesicles, for example, in the introduction of foreign DNA into plant protoplasts in genetic modification experiments4, or in the initiation of virus infection in plant protoplasts5. The amount of DNA which enters fed protoplasts is difficult to determine as is the multiplicity of virus which initiates infection in the poly-L-ornithine (PLO) (ref. 5) and polyethylene glycol (PEG) (ref. 6) procedures. To overcome these limitations of existing methods for the introduction of materials into protoplasts, I report here a vesicle system with the potential to fuse with the negatively charged plasmalemma7. Single membraned (‘hollow’) vesicles were considered more suitable to combine membrane instability with large ‘load’ volume, required to facilitate carriage of large molecules/virus particles into the protoplast and to release their content synchronously within. More... »

PAGES

760-760

Journal

TITLE

Nature

ISSUE

5682

VOLUME

275

Identifiers

URI

http://scigraph.springernature.com/pub.10.1038/275760a0

DOI

http://dx.doi.org/10.1038/275760a0

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1020338406


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