Phenotypic expression in E. coli of a DNA sequence coding for mouse dihydrofolate reductase View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

1978-10

AUTHORS

Annie C. Y. Chang, Jack H. Nunberg, Randal J. Kaufman, Henry A. Erlich, Robert T. Schimke, Stanley N. Cohen

ABSTRACT

The construction and analysis of bacterial plasmids that contain and phenotypically express a mammalian genetic sequence are described. Such plasmids specify a protein that has enzymatic properties, immunological reactivity and molecular size characteristic of the mouse dihydrofolate reductase, and render host cells resistant to the antimetabolic drug trimethoprim.

PAGES

617-624

Journal

TITLE

Nature

ISSUE

5681

VOLUME

275

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  • Identifiers

    URI

    http://scigraph.springernature.com/pub.10.1038/275617a0

    DOI

    http://dx.doi.org/10.1038/275617a0

    DIMENSIONS

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    PUBMED

    https://www.ncbi.nlm.nih.gov/pubmed/360074


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