Does ultraviolet light enhance postreplication repair in mammalian cells? View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

1978-09

AUTHORS

ROBERT B. PAINTER

ABSTRACT

D'AMBROSIO AND SETLOW reported an apparent enhancement of postreplication repair in Chinese hamster V79 cells1. A first exposure of cells to ultraviolet (UV) light was said to make it possible, after re-exposure to UV, for the cells to join the DNA together to form high molecular weight DNA more rapidly than if the cells had not been previously exposed. They irradiated cells with 2.5 J m−2 of UV 2 h before a second exposure to 5.0 J m−2, and then pulse-labelled the cells with 3H-thymidine before chasing for 1 h. When analysed by alkaline sucrose gradient velocity sedimentation, the DNA of these cells showed a profile of radioactivity very different from that of cells irradiated only once with 5.0 J m−2 before pulse-labelling and chasing. For cells irradiated twice the peak of labelled DNA was at a position corresponding to a higher molecular weight than the peak for cells irradiated once. I report here results confirming the data of D'Ambrosio and Setlow, but use of an alternative method of data presentation suggests that interpretations other than the one assumed by D'Ambrosio and Setlow are equally possible or maybe more likely. More... »

PAGES

243-245

Identifiers

URI

http://scigraph.springernature.com/pub.10.1038/275243a0

DOI

http://dx.doi.org/10.1038/275243a0

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1016979654

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/692701


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