Mutagen–oligonucleotide complexes with a bulged base as models for frameshift mutation View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

1978-08

AUTHORS

CHE-HUNG LEE, IGNACIO TINOCO

ABSTRACT

ACRIDINES and ethidium are frameshift mutagens1 which intercalate into DNA and RNA2, and into minihelices3,4 made of complementary dinucleotides. However, this intercalation does not provide an explanation of their mutagenic action. Frameshift mutagenesis requires a bulge of one or two bases in either the parent strand (a deletion mutation), or in the newly synthesised strand (an addition mutation). That is, for frameshift mutation to occur, some bases must remain unpaired and outside the normal double helix during replication. We have prepared minihelices containing such bulged bases stabilised by ethidium. Nuclear magnetic resonance (NMR) studies reported here provide strong evidence that a mixture of the oligonucleotides GpUpG plus CpC in the presence of ethidium produces a minihelix containing two G·C base pairs with the uracil base bulged outside the helix and the ethidium intercalated. Similarly, CpUpG forms minihelices in the presence of ethidium containing bulged bases on both strands. These complexes provide models for the mutagenic action of ethidium. More... »

PAGES

609-610

Identifiers

URI

http://scigraph.springernature.com/pub.10.1038/274609a0

DOI

http://dx.doi.org/10.1038/274609a0

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1045069878

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/672994


Indexing Status Check whether this publication has been indexed by Scopus and Web Of Science using the SN Indexing Status Tool
Incoming Citations Browse incoming citations for this publication using opencitations.net

JSON-LD is the canonical representation for SciGraph data.

TIP: You can open this SciGraph record using an external JSON-LD service: JSON-LD Playground Google SDTT

[
  {
    "@context": "https://springernature.github.io/scigraph/jsonld/sgcontext.json", 
    "about": [
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/06", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Biological Sciences", 
        "type": "DefinedTerm"
      }, 
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/0601", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Biochemistry and Cell Biology", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Circular Dichroism", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Ethidium", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Magnetic Resonance Spectroscopy", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Models, Biological", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Mutation", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Nucleic Acid Conformation", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Oligonucleotides", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Oligoribonucleotides", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Ribose", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Temperature", 
        "type": "DefinedTerm"
      }
    ], 
    "author": [
      {
        "affiliation": {
          "alternateName": "Chemistry Department and Laboratory of Chemical Biodynamics, University of California, 94720, Berkeley, California", 
          "id": "http://www.grid.ac/institutes/grid.47840.3f", 
          "name": [
            "Chemistry Department and Laboratory of Chemical Biodynamics, University of California, 94720, Berkeley, California"
          ], 
          "type": "Organization"
        }, 
        "familyName": "LEE", 
        "givenName": "CHE-HUNG", 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Chemistry Department and Laboratory of Chemical Biodynamics, University of California, 94720, Berkeley, California", 
          "id": "http://www.grid.ac/institutes/grid.47840.3f", 
          "name": [
            "Chemistry Department and Laboratory of Chemical Biodynamics, University of California, 94720, Berkeley, California"
          ], 
          "type": "Organization"
        }, 
        "familyName": "TINOCO", 
        "givenName": "IGNACIO", 
        "id": "sg:person.01001201301.89", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01001201301.89"
        ], 
        "type": "Person"
      }
    ], 
    "datePublished": "1978-08", 
    "datePublishedReg": "1978-08-01", 
    "description": "ACRIDINES and ethidium are frameshift mutagens1 which intercalate into DNA and RNA2, and into minihelices3,4 made of complementary dinucleotides. However, this intercalation does not provide an explanation of their mutagenic action. Frameshift mutagenesis requires a bulge of one or two bases in either the parent strand (a deletion mutation), or in the newly synthesised strand (an addition mutation). That is, for frameshift mutation to occur, some bases must remain unpaired and outside the normal double helix during replication. We have prepared minihelices containing such bulged bases stabilised by ethidium. Nuclear magnetic resonance (NMR) studies reported here provide strong evidence that a mixture of the oligonucleotides GpUpG plus CpC in the presence of ethidium produces a minihelix containing two G\u00b7C base pairs with the uracil base bulged outside the helix and the ethidium intercalated. Similarly, CpUpG forms minihelices in the presence of ethidium containing bulged bases on both strands. These complexes provide models for the mutagenic action of ethidium.", 
    "genre": "article", 
    "id": "sg:pub.10.1038/274609a0", 
    "isAccessibleForFree": false, 
    "isPartOf": [
      {
        "id": "sg:journal.1018957", 
        "issn": [
          "0028-0836", 
          "1476-4687"
        ], 
        "name": "Nature", 
        "publisher": "Springer Nature", 
        "type": "Periodical"
      }, 
      {
        "issueNumber": "5671", 
        "type": "PublicationIssue"
      }, 
      {
        "type": "PublicationVolume", 
        "volumeNumber": "274"
      }
    ], 
    "keywords": [
      "presence of ethidium", 
      "nuclear magnetic resonance studies", 
      "magnetic resonance studies", 
      "resonance studies", 
      "uracil base", 
      "double helix", 
      "bulged base", 
      "ethidium", 
      "complementary dinucleotides", 
      "complexes", 
      "intercalation", 
      "helix", 
      "acridine", 
      "frameshift mutation", 
      "base pairs", 
      "mixture", 
      "strands", 
      "oligonucleotide", 
      "mutagenic action", 
      "parent strand", 
      "presence", 
      "minihelix", 
      "dinucleotide", 
      "frameshift mutagenesis", 
      "minihelices", 
      "mutations", 
      "base", 
      "basis", 
      "RNA2", 
      "mutagenesis", 
      "DNA", 
      "pairs", 
      "strong evidence", 
      "CPC", 
      "replication", 
      "action", 
      "study", 
      "evidence", 
      "explanation", 
      "model", 
      "bulge"
    ], 
    "name": "Mutagen\u2013oligonucleotide complexes with a bulged base as models for frameshift mutation", 
    "pagination": "609-610", 
    "productId": [
      {
        "name": "dimensions_id", 
        "type": "PropertyValue", 
        "value": [
          "pub.1045069878"
        ]
      }, 
      {
        "name": "doi", 
        "type": "PropertyValue", 
        "value": [
          "10.1038/274609a0"
        ]
      }, 
      {
        "name": "pubmed_id", 
        "type": "PropertyValue", 
        "value": [
          "672994"
        ]
      }
    ], 
    "sameAs": [
      "https://doi.org/10.1038/274609a0", 
      "https://app.dimensions.ai/details/publication/pub.1045069878"
    ], 
    "sdDataset": "articles", 
    "sdDatePublished": "2022-05-20T07:17", 
    "sdLicense": "https://scigraph.springernature.com/explorer/license/", 
    "sdPublisher": {
      "name": "Springer Nature - SN SciGraph project", 
      "type": "Organization"
    }, 
    "sdSource": "s3://com-springernature-scigraph/baseset/20220519/entities/gbq_results/article/article_146.jsonl", 
    "type": "ScholarlyArticle", 
    "url": "https://doi.org/10.1038/274609a0"
  }
]
 

Download the RDF metadata as:  json-ld nt turtle xml License info

HOW TO GET THIS DATA PROGRAMMATICALLY:

JSON-LD is a popular format for linked data which is fully compatible with JSON.

curl -H 'Accept: application/ld+json' 'https://scigraph.springernature.com/pub.10.1038/274609a0'

N-Triples is a line-based linked data format ideal for batch operations.

curl -H 'Accept: application/n-triples' 'https://scigraph.springernature.com/pub.10.1038/274609a0'

Turtle is a human-readable linked data format.

curl -H 'Accept: text/turtle' 'https://scigraph.springernature.com/pub.10.1038/274609a0'

RDF/XML is a standard XML format for linked data.

curl -H 'Accept: application/rdf+xml' 'https://scigraph.springernature.com/pub.10.1038/274609a0'


 

This table displays all metadata directly associated to this object as RDF triples.

148 TRIPLES      20 PREDICATES      77 URIs      69 LITERALS      17 BLANK NODES

Subject Predicate Object
1 sg:pub.10.1038/274609a0 schema:about N1c9b11f46da04e85b902eb65750ebac3
2 N21478772cd684569b13460b79fc447f0
3 N3f3ce35ef28e4b83947bbe98737c2b7c
4 N676aa55a46d0436eb82a3eb772b0be77
5 N9633ba702dd3420d83d399af9b298979
6 N9883138216c040db8dca577760e61dec
7 Nb95126c942024830a974664a0789c9b5
8 Nc6a3c3c14b124499a2fd914ecd7ab5f7
9 Neebed3c3569f45529855266f5841ae92
10 Nfd7542ba5d0447a19a2e659fee4a6bc4
11 anzsrc-for:06
12 anzsrc-for:0601
13 schema:author Nc7a1f566088f45d78ba64474f74ffafc
14 schema:datePublished 1978-08
15 schema:datePublishedReg 1978-08-01
16 schema:description ACRIDINES and ethidium are frameshift mutagens1 which intercalate into DNA and RNA2, and into minihelices3,4 made of complementary dinucleotides. However, this intercalation does not provide an explanation of their mutagenic action. Frameshift mutagenesis requires a bulge of one or two bases in either the parent strand (a deletion mutation), or in the newly synthesised strand (an addition mutation). That is, for frameshift mutation to occur, some bases must remain unpaired and outside the normal double helix during replication. We have prepared minihelices containing such bulged bases stabilised by ethidium. Nuclear magnetic resonance (NMR) studies reported here provide strong evidence that a mixture of the oligonucleotides GpUpG plus CpC in the presence of ethidium produces a minihelix containing two G·C base pairs with the uracil base bulged outside the helix and the ethidium intercalated. Similarly, CpUpG forms minihelices in the presence of ethidium containing bulged bases on both strands. These complexes provide models for the mutagenic action of ethidium.
17 schema:genre article
18 schema:isAccessibleForFree false
19 schema:isPartOf N05839bedee3c4881ba62e00e02575f05
20 Nfed652c24bcc4e87b25b6f7e026e78ea
21 sg:journal.1018957
22 schema:keywords CPC
23 DNA
24 RNA2
25 acridine
26 action
27 base
28 base pairs
29 basis
30 bulge
31 bulged base
32 complementary dinucleotides
33 complexes
34 dinucleotide
35 double helix
36 ethidium
37 evidence
38 explanation
39 frameshift mutagenesis
40 frameshift mutation
41 helix
42 intercalation
43 magnetic resonance studies
44 minihelices
45 minihelix
46 mixture
47 model
48 mutagenesis
49 mutagenic action
50 mutations
51 nuclear magnetic resonance studies
52 oligonucleotide
53 pairs
54 parent strand
55 presence
56 presence of ethidium
57 replication
58 resonance studies
59 strands
60 strong evidence
61 study
62 uracil base
63 schema:name Mutagen–oligonucleotide complexes with a bulged base as models for frameshift mutation
64 schema:pagination 609-610
65 schema:productId N2665351a833f4705bfb25a76fcb2dbbd
66 Na625706fca4b4380a4c986134567e131
67 Nf752f31beb58476abcb1738af0d3dfaa
68 schema:sameAs https://app.dimensions.ai/details/publication/pub.1045069878
69 https://doi.org/10.1038/274609a0
70 schema:sdDatePublished 2022-05-20T07:17
71 schema:sdLicense https://scigraph.springernature.com/explorer/license/
72 schema:sdPublisher N2d5492865e0649afbeed1e258f6d9ba3
73 schema:url https://doi.org/10.1038/274609a0
74 sgo:license sg:explorer/license/
75 sgo:sdDataset articles
76 rdf:type schema:ScholarlyArticle
77 N05839bedee3c4881ba62e00e02575f05 schema:volumeNumber 274
78 rdf:type schema:PublicationVolume
79 N1c9b11f46da04e85b902eb65750ebac3 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
80 schema:name Nucleic Acid Conformation
81 rdf:type schema:DefinedTerm
82 N21478772cd684569b13460b79fc447f0 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
83 schema:name Ribose
84 rdf:type schema:DefinedTerm
85 N2665351a833f4705bfb25a76fcb2dbbd schema:name dimensions_id
86 schema:value pub.1045069878
87 rdf:type schema:PropertyValue
88 N2d5492865e0649afbeed1e258f6d9ba3 schema:name Springer Nature - SN SciGraph project
89 rdf:type schema:Organization
90 N2e5c2a7ec9c7405e9faf040e4062f6fe rdf:first sg:person.01001201301.89
91 rdf:rest rdf:nil
92 N3f3ce35ef28e4b83947bbe98737c2b7c schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
93 schema:name Temperature
94 rdf:type schema:DefinedTerm
95 N676aa55a46d0436eb82a3eb772b0be77 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
96 schema:name Oligonucleotides
97 rdf:type schema:DefinedTerm
98 N9633ba702dd3420d83d399af9b298979 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
99 schema:name Oligoribonucleotides
100 rdf:type schema:DefinedTerm
101 N9883138216c040db8dca577760e61dec schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
102 schema:name Mutation
103 rdf:type schema:DefinedTerm
104 Na625706fca4b4380a4c986134567e131 schema:name doi
105 schema:value 10.1038/274609a0
106 rdf:type schema:PropertyValue
107 Nb95126c942024830a974664a0789c9b5 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
108 schema:name Ethidium
109 rdf:type schema:DefinedTerm
110 Nc6a3c3c14b124499a2fd914ecd7ab5f7 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
111 schema:name Magnetic Resonance Spectroscopy
112 rdf:type schema:DefinedTerm
113 Nc7a1f566088f45d78ba64474f74ffafc rdf:first Ncbd1342105e04704b7b8e3430422104a
114 rdf:rest N2e5c2a7ec9c7405e9faf040e4062f6fe
115 Ncbd1342105e04704b7b8e3430422104a schema:affiliation grid-institutes:grid.47840.3f
116 schema:familyName LEE
117 schema:givenName CHE-HUNG
118 rdf:type schema:Person
119 Neebed3c3569f45529855266f5841ae92 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
120 schema:name Circular Dichroism
121 rdf:type schema:DefinedTerm
122 Nf752f31beb58476abcb1738af0d3dfaa schema:name pubmed_id
123 schema:value 672994
124 rdf:type schema:PropertyValue
125 Nfd7542ba5d0447a19a2e659fee4a6bc4 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
126 schema:name Models, Biological
127 rdf:type schema:DefinedTerm
128 Nfed652c24bcc4e87b25b6f7e026e78ea schema:issueNumber 5671
129 rdf:type schema:PublicationIssue
130 anzsrc-for:06 schema:inDefinedTermSet anzsrc-for:
131 schema:name Biological Sciences
132 rdf:type schema:DefinedTerm
133 anzsrc-for:0601 schema:inDefinedTermSet anzsrc-for:
134 schema:name Biochemistry and Cell Biology
135 rdf:type schema:DefinedTerm
136 sg:journal.1018957 schema:issn 0028-0836
137 1476-4687
138 schema:name Nature
139 schema:publisher Springer Nature
140 rdf:type schema:Periodical
141 sg:person.01001201301.89 schema:affiliation grid-institutes:grid.47840.3f
142 schema:familyName TINOCO
143 schema:givenName IGNACIO
144 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01001201301.89
145 rdf:type schema:Person
146 grid-institutes:grid.47840.3f schema:alternateName Chemistry Department and Laboratory of Chemical Biodynamics, University of California, 94720, Berkeley, California
147 schema:name Chemistry Department and Laboratory of Chemical Biodynamics, University of California, 94720, Berkeley, California
148 rdf:type schema:Organization
 




Preview window. Press ESC to close (or click here)


...