Plasmid incompatibility: cloning analysis of an inc FII determinant of R6-5 View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

1978-05

AUTHORS

Kenneth N. Timmis, Isabel Andrés, Patrick M. Slocombe

ABSTRACT

SalI and PstI restriction endonuclease-generated DNA fragments that specify an FII-type incompatibility function (incFII) of the low copy number antibiotic resistance plasmid R6-5 have been cloned in the high copy number pBR322 plasmid vector. A 1-kilobase DNA sequence that contains this incFII determinant has been identified and is shown to have coordinates of 95.5 and 96.5 kilobases on the R6-5 plasmid physical map. Expression of incompatibility by the cloned PstI fragment depends on its orientation within the vector molecule. The behaviour of pBR322-incFII hybrid plasmids suggests that plasmid replication control is not the only mechanism that can cause incompatibility between two plasmids. More... »

PAGES

27-32

Identifiers

URI

http://scigraph.springernature.com/pub.10.1038/273027a0

DOI

http://dx.doi.org/10.1038/273027a0

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1026269359

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/692666


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