Direct measurement of intracellular pH during metabolic derepression of the sea urchin egg View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

1978-03

AUTHORS

SHELDON S. SHEN, RICHA A. STEINHAT

ABSTRACT

SEA URCHIN eggs are shed in a metabolically repressed state and are derepressed by fertilisation. This derepression occurs in two phases, the first of which is caused by an intracellular release of Ca2+ (refs 1,2). It has been postulated that the second phase of derepression is accomplished by an increase of intracellular pH3–5. Furthermore, attempts to measure intracellular pH by the use of cell homogenates have supported the idea that intracellular pH is increased during egg activation5,6. We report here direct measurements of intracellular pH of sea urchin eggs during fertilisation and activation by ammonia. These measurements demonstrate the postulated increase of intracellular pH resulting from fertilisation or exposure to NH4Cl, although the changes in pH differ from those reported in homogenates and seem to be regulated in a more complex manner than originally anticipated. More... »

PAGES

253-254

Identifiers

URI

http://scigraph.springernature.com/pub.10.1038/272253a0

DOI

http://dx.doi.org/10.1038/272253a0

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1044770975

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/24185


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