The invertible segment of bacteriophage Mu DNA determines the adsorption properties of Mu particles View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

1978-02

AUTHORS

A. I. BUKHARI, LINDA AROSIO

ABSTRACT

INTEREST in bacteriophage Mu stems from the highly promiscuous insertion of its DNA into the genome of its host bacterium Escherichia coli (see ref. 1 for review). There are two characterstic features of Mu DNA: first, mature Mu DNA contains heterogeneous host sequences at both ends2,3, and second, near the right end, the S end, of Mu DNA there is a 3,000 base pair sequence that can undergo inversion. The structure of Mu DNA is diagrammed in Fig. 1. The invertible sequence, called the G segment, is remarkable in that it is also found in bacteriophage P1 (ref. 4). The inversion of the G segment in Mu occurs in the prophage state and is independent of the recA function of the host5. According to Hsu and Davidson6, the inversion occurs by recombination between identical but inverted sequences of about 50 base pairs flanking the G segment. Allet and Bukhari7 have presented evidence that a Mu function, located within or close to the G segment, is required for the inversion reaction. When Mu particles are grown by induction of a lysogen, about half of the particles contain DNA with one orientation (referred to as the + or flip orientation) and the rest of the particles have the G segment in the reverse orientation (the − or flop orientation). However, when Mu particles are grown by infection, almost all of the particles contain DNA with the flip orientation. The predominance of the flip orientation is obtained even if the phage lysate used for infection contained equal numbers of the flip and the flop orientations. We show here that the predominance of the flip orientation after infection results from the inability of the flop containing particles to adsorb properly to the bacterial cells. More... »

PAGES

575

References to SciGraph publications

Identifiers

URI

http://scigraph.springernature.com/pub.10.1038/271575a0

DOI

http://dx.doi.org/10.1038/271575a0

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1017101998

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/622195


Indexing Status Check whether this publication has been indexed by Scopus and Web Of Science using the SN Indexing Status Tool
Incoming Citations Browse incoming citations for this publication using opencitations.net

JSON-LD is the canonical representation for SciGraph data.

TIP: You can open this SciGraph record using an external JSON-LD service: JSON-LD Playground Google SDTT

[
  {
    "@context": "https://springernature.github.io/scigraph/jsonld/sgcontext.json", 
    "about": [
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/1108", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Medical Microbiology", 
        "type": "DefinedTerm"
      }, 
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/11", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Medical and Health Sciences", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Adsorption", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Base Sequence", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Coliphages", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "DNA Replication", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "DNA, Viral", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Virus Replication", 
        "type": "DefinedTerm"
      }
    ], 
    "author": [
      {
        "familyName": "BUKHARI", 
        "givenName": "A. I.", 
        "type": "Person"
      }, 
      {
        "familyName": "AROSIO", 
        "givenName": "LINDA", 
        "type": "Person"
      }
    ], 
    "citation": [
      {
        "id": "sg:pub.10.1038/264580a0", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1002220482", 
          "https://doi.org/10.1038/264580a0"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/271577a0", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1009923423", 
          "https://doi.org/10.1038/271577a0"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/271577a0", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1009923423", 
          "https://doi.org/10.1038/271577a0"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/0042-6822(73)90385-1", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1022448629"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/s0022-2836(75)80083-0", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1033296365"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/0042-6822(76)90148-3", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1038598612"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1073/pnas.74.8.3143", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1039779096"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/271573a0", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1047207553", 
          "https://doi.org/10.1038/271573a0"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/271573a0", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1047207553", 
          "https://doi.org/10.1038/271573a0"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/0042-6822(74)90157-3", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1048975178"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/0022-2836(75)90307-1", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1049446378"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1073/pnas.69.10.2823", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1049983340"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1146/annurev.ge.10.120176.002133", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1053439662"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1126/science.322276", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1062600445"
        ], 
        "type": "CreativeWork"
      }
    ], 
    "datePublished": "1978-02", 
    "datePublishedReg": "1978-02-01", 
    "description": "INTEREST in bacteriophage Mu stems from the highly promiscuous insertion of its DNA into the genome of its host bacterium Escherichia coli (see ref. 1 for review). There are two characterstic features of Mu DNA: first, mature Mu DNA contains heterogeneous host sequences at both ends2,3, and second, near the right end, the S end, of Mu DNA there is a 3,000 base pair sequence that can undergo inversion. The structure of Mu DNA is diagrammed in Fig. 1. The invertible sequence, called the G segment, is remarkable in that it is also found in bacteriophage P1 (ref. 4). The inversion of the G segment in Mu occurs in the prophage state and is independent of the recA function of the host5. According to Hsu and Davidson6, the inversion occurs by recombination between identical but inverted sequences of about 50 base pairs flanking the G segment. Allet and Bukhari7 have presented evidence that a Mu function, located within or close to the G segment, is required for the inversion reaction. When Mu particles are grown by induction of a lysogen, about half of the particles contain DNA with one orientation (referred to as the + or flip orientation) and the rest of the particles have the G segment in the reverse orientation (the \u2212 or flop orientation). However, when Mu particles are grown by infection, almost all of the particles contain DNA with the flip orientation. The predominance of the flip orientation is obtained even if the phage lysate used for infection contained equal numbers of the flip and the flop orientations. We show here that the predominance of the flip orientation after infection results from the inability of the flop containing particles to adsorb properly to the bacterial cells.", 
    "genre": "research_article", 
    "id": "sg:pub.10.1038/271575a0", 
    "inLanguage": [
      "en"
    ], 
    "isAccessibleForFree": false, 
    "isPartOf": [
      {
        "id": "sg:journal.1018957", 
        "issn": [
          "0090-0028", 
          "1476-4687"
        ], 
        "name": "Nature", 
        "type": "Periodical"
      }, 
      {
        "issueNumber": "5645", 
        "type": "PublicationIssue"
      }, 
      {
        "type": "PublicationVolume", 
        "volumeNumber": "271"
      }
    ], 
    "name": "The invertible segment of bacteriophage Mu DNA determines the adsorption properties of Mu particles", 
    "pagination": "575", 
    "productId": [
      {
        "name": "readcube_id", 
        "type": "PropertyValue", 
        "value": [
          "517b47fd41006ab7048fedc772d3ae59116d34129d651470b6928feaa56d0490"
        ]
      }, 
      {
        "name": "pubmed_id", 
        "type": "PropertyValue", 
        "value": [
          "622195"
        ]
      }, 
      {
        "name": "nlm_unique_id", 
        "type": "PropertyValue", 
        "value": [
          "0410462"
        ]
      }, 
      {
        "name": "doi", 
        "type": "PropertyValue", 
        "value": [
          "10.1038/271575a0"
        ]
      }, 
      {
        "name": "dimensions_id", 
        "type": "PropertyValue", 
        "value": [
          "pub.1017101998"
        ]
      }
    ], 
    "sameAs": [
      "https://doi.org/10.1038/271575a0", 
      "https://app.dimensions.ai/details/publication/pub.1017101998"
    ], 
    "sdDataset": "articles", 
    "sdDatePublished": "2019-04-11T11:54", 
    "sdLicense": "https://scigraph.springernature.com/explorer/license/", 
    "sdPublisher": {
      "name": "Springer Nature - SN SciGraph project", 
      "type": "Organization"
    }, 
    "sdSource": "s3://com-uberresearch-data-dimensions-target-20181106-alternative/cleanup/v134/2549eaecd7973599484d7c17b260dba0a4ecb94b/merge/v9/a6c9fde33151104705d4d7ff012ea9563521a3ce/jats-lookup/v90/0000000359_0000000359/records_29203_00000000.jsonl", 
    "type": "ScholarlyArticle", 
    "url": "https://www.nature.com/articles/271575a0"
  }
]
 

Download the RDF metadata as:  json-ld nt turtle xml License info

HOW TO GET THIS DATA PROGRAMMATICALLY:

JSON-LD is a popular format for linked data which is fully compatible with JSON.

curl -H 'Accept: application/ld+json' 'https://scigraph.springernature.com/pub.10.1038/271575a0'

N-Triples is a line-based linked data format ideal for batch operations.

curl -H 'Accept: application/n-triples' 'https://scigraph.springernature.com/pub.10.1038/271575a0'

Turtle is a human-readable linked data format.

curl -H 'Accept: text/turtle' 'https://scigraph.springernature.com/pub.10.1038/271575a0'

RDF/XML is a standard XML format for linked data.

curl -H 'Accept: application/rdf+xml' 'https://scigraph.springernature.com/pub.10.1038/271575a0'


 

This table displays all metadata directly associated to this object as RDF triples.

132 TRIPLES      21 PREDICATES      47 URIs      27 LITERALS      15 BLANK NODES

Subject Predicate Object
1 sg:pub.10.1038/271575a0 schema:about N193dc2527d9c416cae4a7e919a86cfa4
2 N2221429e237b46b89b0ed70ef8250ac3
3 N43aac29694fb4cb2bcf6601b42a9046c
4 N7e095a0824f541a7bdcca3c2b2a34b26
5 N84f1c22a59fb4d98b4d512d1e86bc905
6 Nd8418b1d72194fb4920b5e03f5764978
7 anzsrc-for:11
8 anzsrc-for:1108
9 schema:author Nd5282ff0177240bd83e72493fc372123
10 schema:citation sg:pub.10.1038/264580a0
11 sg:pub.10.1038/271573a0
12 sg:pub.10.1038/271577a0
13 https://doi.org/10.1016/0022-2836(75)90307-1
14 https://doi.org/10.1016/0042-6822(73)90385-1
15 https://doi.org/10.1016/0042-6822(74)90157-3
16 https://doi.org/10.1016/0042-6822(76)90148-3
17 https://doi.org/10.1016/s0022-2836(75)80083-0
18 https://doi.org/10.1073/pnas.69.10.2823
19 https://doi.org/10.1073/pnas.74.8.3143
20 https://doi.org/10.1126/science.322276
21 https://doi.org/10.1146/annurev.ge.10.120176.002133
22 schema:datePublished 1978-02
23 schema:datePublishedReg 1978-02-01
24 schema:description INTEREST in bacteriophage Mu stems from the highly promiscuous insertion of its DNA into the genome of its host bacterium Escherichia coli (see ref. 1 for review). There are two characterstic features of Mu DNA: first, mature Mu DNA contains heterogeneous host sequences at both ends2,3, and second, near the right end, the S end, of Mu DNA there is a 3,000 base pair sequence that can undergo inversion. The structure of Mu DNA is diagrammed in Fig. 1. The invertible sequence, called the G segment, is remarkable in that it is also found in bacteriophage P1 (ref. 4). The inversion of the G segment in Mu occurs in the prophage state and is independent of the recA function of the host5. According to Hsu and Davidson6, the inversion occurs by recombination between identical but inverted sequences of about 50 base pairs flanking the G segment. Allet and Bukhari7 have presented evidence that a Mu function, located within or close to the G segment, is required for the inversion reaction. When Mu particles are grown by induction of a lysogen, about half of the particles contain DNA with one orientation (referred to as the + or flip orientation) and the rest of the particles have the G segment in the reverse orientation (the − or flop orientation). However, when Mu particles are grown by infection, almost all of the particles contain DNA with the flip orientation. The predominance of the flip orientation is obtained even if the phage lysate used for infection contained equal numbers of the flip and the flop orientations. We show here that the predominance of the flip orientation after infection results from the inability of the flop containing particles to adsorb properly to the bacterial cells.
25 schema:genre research_article
26 schema:inLanguage en
27 schema:isAccessibleForFree false
28 schema:isPartOf Nca4d113fcf38426d8d7da18d3ae02f83
29 Nf95d2ceceea241f69e4b4c40dd2c1247
30 sg:journal.1018957
31 schema:name The invertible segment of bacteriophage Mu DNA determines the adsorption properties of Mu particles
32 schema:pagination 575
33 schema:productId N1a1cebf829b7452f92d7cc485013f983
34 N43720187365c499e97f740c97273125b
35 Nb823942401694a959e0afd068dfc12da
36 Nc5b95a100e6642489d1adfea98091a62
37 Nd1a488cf7a4c40ccaa4e0551b2e8977b
38 schema:sameAs https://app.dimensions.ai/details/publication/pub.1017101998
39 https://doi.org/10.1038/271575a0
40 schema:sdDatePublished 2019-04-11T11:54
41 schema:sdLicense https://scigraph.springernature.com/explorer/license/
42 schema:sdPublisher Nb7a84337ac01420b80ef56e5f8a9bec5
43 schema:url https://www.nature.com/articles/271575a0
44 sgo:license sg:explorer/license/
45 sgo:sdDataset articles
46 rdf:type schema:ScholarlyArticle
47 N193dc2527d9c416cae4a7e919a86cfa4 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
48 schema:name Adsorption
49 rdf:type schema:DefinedTerm
50 N1a1cebf829b7452f92d7cc485013f983 schema:name pubmed_id
51 schema:value 622195
52 rdf:type schema:PropertyValue
53 N2221429e237b46b89b0ed70ef8250ac3 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
54 schema:name DNA, Viral
55 rdf:type schema:DefinedTerm
56 N43720187365c499e97f740c97273125b schema:name dimensions_id
57 schema:value pub.1017101998
58 rdf:type schema:PropertyValue
59 N43aac29694fb4cb2bcf6601b42a9046c schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
60 schema:name Base Sequence
61 rdf:type schema:DefinedTerm
62 N7e095a0824f541a7bdcca3c2b2a34b26 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
63 schema:name DNA Replication
64 rdf:type schema:DefinedTerm
65 N84f1c22a59fb4d98b4d512d1e86bc905 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
66 schema:name Coliphages
67 rdf:type schema:DefinedTerm
68 N901b310f94af4330934faea955639045 schema:familyName BUKHARI
69 schema:givenName A. I.
70 rdf:type schema:Person
71 Nb606bb110df44827bbbd8dc714fdeec0 schema:familyName AROSIO
72 schema:givenName LINDA
73 rdf:type schema:Person
74 Nb7a84337ac01420b80ef56e5f8a9bec5 schema:name Springer Nature - SN SciGraph project
75 rdf:type schema:Organization
76 Nb823942401694a959e0afd068dfc12da schema:name nlm_unique_id
77 schema:value 0410462
78 rdf:type schema:PropertyValue
79 Nc5b95a100e6642489d1adfea98091a62 schema:name readcube_id
80 schema:value 517b47fd41006ab7048fedc772d3ae59116d34129d651470b6928feaa56d0490
81 rdf:type schema:PropertyValue
82 Nca4d113fcf38426d8d7da18d3ae02f83 schema:issueNumber 5645
83 rdf:type schema:PublicationIssue
84 Nd1a488cf7a4c40ccaa4e0551b2e8977b schema:name doi
85 schema:value 10.1038/271575a0
86 rdf:type schema:PropertyValue
87 Nd5282ff0177240bd83e72493fc372123 rdf:first N901b310f94af4330934faea955639045
88 rdf:rest Nf552db7ce55147559c8bb3fe6ac11823
89 Nd8418b1d72194fb4920b5e03f5764978 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
90 schema:name Virus Replication
91 rdf:type schema:DefinedTerm
92 Nf552db7ce55147559c8bb3fe6ac11823 rdf:first Nb606bb110df44827bbbd8dc714fdeec0
93 rdf:rest rdf:nil
94 Nf95d2ceceea241f69e4b4c40dd2c1247 schema:volumeNumber 271
95 rdf:type schema:PublicationVolume
96 anzsrc-for:11 schema:inDefinedTermSet anzsrc-for:
97 schema:name Medical and Health Sciences
98 rdf:type schema:DefinedTerm
99 anzsrc-for:1108 schema:inDefinedTermSet anzsrc-for:
100 schema:name Medical Microbiology
101 rdf:type schema:DefinedTerm
102 sg:journal.1018957 schema:issn 0090-0028
103 1476-4687
104 schema:name Nature
105 rdf:type schema:Periodical
106 sg:pub.10.1038/264580a0 schema:sameAs https://app.dimensions.ai/details/publication/pub.1002220482
107 https://doi.org/10.1038/264580a0
108 rdf:type schema:CreativeWork
109 sg:pub.10.1038/271573a0 schema:sameAs https://app.dimensions.ai/details/publication/pub.1047207553
110 https://doi.org/10.1038/271573a0
111 rdf:type schema:CreativeWork
112 sg:pub.10.1038/271577a0 schema:sameAs https://app.dimensions.ai/details/publication/pub.1009923423
113 https://doi.org/10.1038/271577a0
114 rdf:type schema:CreativeWork
115 https://doi.org/10.1016/0022-2836(75)90307-1 schema:sameAs https://app.dimensions.ai/details/publication/pub.1049446378
116 rdf:type schema:CreativeWork
117 https://doi.org/10.1016/0042-6822(73)90385-1 schema:sameAs https://app.dimensions.ai/details/publication/pub.1022448629
118 rdf:type schema:CreativeWork
119 https://doi.org/10.1016/0042-6822(74)90157-3 schema:sameAs https://app.dimensions.ai/details/publication/pub.1048975178
120 rdf:type schema:CreativeWork
121 https://doi.org/10.1016/0042-6822(76)90148-3 schema:sameAs https://app.dimensions.ai/details/publication/pub.1038598612
122 rdf:type schema:CreativeWork
123 https://doi.org/10.1016/s0022-2836(75)80083-0 schema:sameAs https://app.dimensions.ai/details/publication/pub.1033296365
124 rdf:type schema:CreativeWork
125 https://doi.org/10.1073/pnas.69.10.2823 schema:sameAs https://app.dimensions.ai/details/publication/pub.1049983340
126 rdf:type schema:CreativeWork
127 https://doi.org/10.1073/pnas.74.8.3143 schema:sameAs https://app.dimensions.ai/details/publication/pub.1039779096
128 rdf:type schema:CreativeWork
129 https://doi.org/10.1126/science.322276 schema:sameAs https://app.dimensions.ai/details/publication/pub.1062600445
130 rdf:type schema:CreativeWork
131 https://doi.org/10.1146/annurev.ge.10.120176.002133 schema:sameAs https://app.dimensions.ai/details/publication/pub.1053439662
132 rdf:type schema:CreativeWork
 




Preview window. Press ESC to close (or click here)


...