Two enzymes are required for strand incision in repair of alkylated DNA View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

1977-10

AUTHORS

JACQUES LAVAL

ABSTRACT

THE excision-repair model for DNA repair involves the incision by an endonuclease of the phosphodiester chain adjacent or close to the damage and subsequent excision by an exonuclease of the defective nucleotide(s) (for review see ref. 1). Specific endonuclease for ultraviolet-induced damage1 and for apurinic sites have been isolated associated with2,3, or without an exonuclease activity (refs 4–6 and J.L. and L. Grossman, in preparation). Another class of enzymes does not incise DNA, but recognises and excises defective bases as uracil in the DNA. Belonging to this class of DNA N-glycosidases, is the enzyme described here which excises 3-methyladenine (3-MeAde) from alkylated DNA. This base excision repair is a preparatory step for the nucleotide excision repair describedabove. It has been claimed that endonuclease II of Escherichia coli8 both excises the alkylated bases 3-MeAde and O6-methylguanine (O6-MeGua) and incises DNA atapurinic sites. This enzyme also recognises a number of additional substrates9–11. To establish the (precise mechanism of incision of alkylated DNA we have purified two enzymes from Micrococcus luteus, a DNA N-glycosidase, which excises 3-MeAde, and an endonuclease for apurinic sites. We report here a reconstruction experiment showing that incision of DNA treated with carcinogenic and mutagenic compound methyl methanesulphonate (MMS) is a two-step mechanism involving the sequential action of these two enzymes. More... »

PAGES

829-832

References to SciGraph publications

Identifiers

URI

http://scigraph.springernature.com/pub.10.1038/269829a0

DOI

http://dx.doi.org/10.1038/269829a0

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1034421803

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/927511


Indexing Status Check whether this publication has been indexed by Scopus and Web Of Science using the SN Indexing Status Tool
Incoming Citations Browse incoming citations for this publication using opencitations.net

JSON-LD is the canonical representation for SciGraph data.

TIP: You can open this SciGraph record using an external JSON-LD service: JSON-LD Playground Google SDTT

[
  {
    "@context": "https://springernature.github.io/scigraph/jsonld/sgcontext.json", 
    "about": [
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/06", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Biological Sciences", 
        "type": "DefinedTerm"
      }, 
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/0601", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Biochemistry and Cell Biology", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Apurinic Acid", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "DNA", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "DNA Repair", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "DNA, Bacterial", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "DNA, Superhelical", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "DNA, Viral", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Endonucleases", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Glycoside Hydrolases", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Methylation", 
        "type": "DefinedTerm"
      }
    ], 
    "author": [
      {
        "affiliation": {
          "alternateName": "Laboratoire Associ\u00e9 n 147 CNRS et Unit\u00e9 147 INSERM, Institut Gustave-Roussy, 94800, Villejuif, France", 
          "id": "http://www.grid.ac/institutes/grid.14925.3b", 
          "name": [
            "Laboratoire Associ\u00e9 n 147 CNRS et Unit\u00e9 147 INSERM, Institut Gustave-Roussy, 94800, Villejuif, France"
          ], 
          "type": "Organization"
        }, 
        "familyName": "LAVAL", 
        "givenName": "JACQUES", 
        "id": "sg:person.0710305630.49", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0710305630.49"
        ], 
        "type": "Person"
      }
    ], 
    "citation": [
      {
        "id": "sg:pub.10.1038/258544a0", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1008417712", 
          "https://doi.org/10.1038/258544a0"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/259064a0", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1018362577", 
          "https://doi.org/10.1038/259064a0"
        ], 
        "type": "CreativeWork"
      }
    ], 
    "datePublished": "1977-10", 
    "datePublishedReg": "1977-10-01", 
    "description": "THE excision-repair model for DNA repair involves the incision by an endonuclease of the phosphodiester chain adjacent or close to the damage and subsequent excision by an exonuclease of the defective nucleotide(s) (for review see ref. 1). Specific endonuclease for ultraviolet-induced damage1 and for apurinic sites have been isolated associated with2,3, or without an exonuclease activity (refs 4\u20136 and J.L. and L. Grossman, in preparation). Another class of enzymes does not incise DNA, but recognises and excises defective bases as uracil in the DNA. Belonging to this class of DNA N-glycosidases, is the enzyme described here which excises 3-methyladenine (3-MeAde) from alkylated DNA. This base excision repair is a preparatory step for the nucleotide excision repair describedabove. It has been claimed that endonuclease II of Escherichia coli8 both excises the alkylated bases 3-MeAde and O6-methylguanine (O6-MeGua) and incises DNA atapurinic sites. This enzyme also recognises a number of additional substrates9\u201311. To establish the (precise mechanism of incision of alkylated DNA we have purified two enzymes from Micrococcus luteus, a DNA N-glycosidase, which excises 3-MeAde, and an endonuclease for apurinic sites. We report here a reconstruction experiment showing that incision of DNA treated with carcinogenic and mutagenic compound methyl methanesulphonate (MMS) is a two-step mechanism involving the sequential action of these two enzymes.", 
    "genre": "article", 
    "id": "sg:pub.10.1038/269829a0", 
    "isAccessibleForFree": false, 
    "isPartOf": [
      {
        "id": "sg:journal.1018957", 
        "issn": [
          "0028-0836", 
          "1476-4687"
        ], 
        "name": "Nature", 
        "publisher": "Springer Nature", 
        "type": "Periodical"
      }, 
      {
        "issueNumber": "5631", 
        "type": "PublicationIssue"
      }, 
      {
        "type": "PublicationVolume", 
        "volumeNumber": "269"
      }
    ], 
    "keywords": [
      "class of enzymes", 
      "base excision repair", 
      "N-glycosidases", 
      "DNA repair", 
      "excision repair", 
      "endonuclease II", 
      "exonuclease activity", 
      "specific endonuclease", 
      "DNA", 
      "O6-methylguanine", 
      "apurinic sites", 
      "enzyme", 
      "endonuclease", 
      "phosphodiester chain", 
      "exonuclease", 
      "sites", 
      "repair", 
      "subsequent excision", 
      "excise", 
      "uracil", 
      "basis", 
      "activity", 
      "damage", 
      "chain", 
      "class", 
      "preparatory step", 
      "step", 
      "recognises", 
      "number", 
      "model", 
      "excision", 
      "incision"
    ], 
    "name": "Two enzymes are required for strand incision in repair of alkylated DNA", 
    "pagination": "829-832", 
    "productId": [
      {
        "name": "dimensions_id", 
        "type": "PropertyValue", 
        "value": [
          "pub.1034421803"
        ]
      }, 
      {
        "name": "doi", 
        "type": "PropertyValue", 
        "value": [
          "10.1038/269829a0"
        ]
      }, 
      {
        "name": "pubmed_id", 
        "type": "PropertyValue", 
        "value": [
          "927511"
        ]
      }
    ], 
    "sameAs": [
      "https://doi.org/10.1038/269829a0", 
      "https://app.dimensions.ai/details/publication/pub.1034421803"
    ], 
    "sdDataset": "articles", 
    "sdDatePublished": "2022-05-20T07:17", 
    "sdLicense": "https://scigraph.springernature.com/explorer/license/", 
    "sdPublisher": {
      "name": "Springer Nature - SN SciGraph project", 
      "type": "Organization"
    }, 
    "sdSource": "s3://com-springernature-scigraph/baseset/20220519/entities/gbq_results/article/article_131.jsonl", 
    "type": "ScholarlyArticle", 
    "url": "https://doi.org/10.1038/269829a0"
  }
]
 

Download the RDF metadata as:  json-ld nt turtle xml License info

HOW TO GET THIS DATA PROGRAMMATICALLY:

JSON-LD is a popular format for linked data which is fully compatible with JSON.

curl -H 'Accept: application/ld+json' 'https://scigraph.springernature.com/pub.10.1038/269829a0'

N-Triples is a line-based linked data format ideal for batch operations.

curl -H 'Accept: application/n-triples' 'https://scigraph.springernature.com/pub.10.1038/269829a0'

Turtle is a human-readable linked data format.

curl -H 'Accept: text/turtle' 'https://scigraph.springernature.com/pub.10.1038/269829a0'

RDF/XML is a standard XML format for linked data.

curl -H 'Accept: application/rdf+xml' 'https://scigraph.springernature.com/pub.10.1038/269829a0'


 

This table displays all metadata directly associated to this object as RDF triples.

137 TRIPLES      21 PREDICATES      69 URIs      59 LITERALS      16 BLANK NODES

Subject Predicate Object
1 sg:pub.10.1038/269829a0 schema:about N2368ef1de13c4968a7014b460193b43a
2 N27325796273c4fd8b0e1a600a46715f8
3 N324b13a328974ffcb28dcf4bb28c15dc
4 N6439ceb590a94c13b008098b96613f41
5 N7f94f9367ce74dca8cdc2d1f57cd710e
6 N94f579b03aa5452aa6a4c780635bfc9e
7 Nad64da4cf790401e8049b995db5176cc
8 Nc8725c7c8f424f02af9b22c008c6dcb9
9 Ne488258ee9bd4e7dbaa6f1aa8381d5d6
10 anzsrc-for:06
11 anzsrc-for:0601
12 schema:author N9fbc7a047c194583a3d3e1fc5d6ad595
13 schema:citation sg:pub.10.1038/258544a0
14 sg:pub.10.1038/259064a0
15 schema:datePublished 1977-10
16 schema:datePublishedReg 1977-10-01
17 schema:description THE excision-repair model for DNA repair involves the incision by an endonuclease of the phosphodiester chain adjacent or close to the damage and subsequent excision by an exonuclease of the defective nucleotide(s) (for review see ref. 1). Specific endonuclease for ultraviolet-induced damage1 and for apurinic sites have been isolated associated with2,3, or without an exonuclease activity (refs 4–6 and J.L. and L. Grossman, in preparation). Another class of enzymes does not incise DNA, but recognises and excises defective bases as uracil in the DNA. Belonging to this class of DNA N-glycosidases, is the enzyme described here which excises 3-methyladenine (3-MeAde) from alkylated DNA. This base excision repair is a preparatory step for the nucleotide excision repair describedabove. It has been claimed that endonuclease II of Escherichia coli8 both excises the alkylated bases 3-MeAde and O6-methylguanine (O6-MeGua) and incises DNA atapurinic sites. This enzyme also recognises a number of additional substrates9–11. To establish the (precise mechanism of incision of alkylated DNA we have purified two enzymes from Micrococcus luteus, a DNA N-glycosidase, which excises 3-MeAde, and an endonuclease for apurinic sites. We report here a reconstruction experiment showing that incision of DNA treated with carcinogenic and mutagenic compound methyl methanesulphonate (MMS) is a two-step mechanism involving the sequential action of these two enzymes.
18 schema:genre article
19 schema:isAccessibleForFree false
20 schema:isPartOf N2192ff03972d4d99b1b5c2c7e41b059d
21 Nff28eab7d5664010ad485e11f0bc9b31
22 sg:journal.1018957
23 schema:keywords DNA
24 DNA repair
25 N-glycosidases
26 O6-methylguanine
27 activity
28 apurinic sites
29 base excision repair
30 basis
31 chain
32 class
33 class of enzymes
34 damage
35 endonuclease
36 endonuclease II
37 enzyme
38 excise
39 excision
40 excision repair
41 exonuclease
42 exonuclease activity
43 incision
44 model
45 number
46 phosphodiester chain
47 preparatory step
48 recognises
49 repair
50 sites
51 specific endonuclease
52 step
53 subsequent excision
54 uracil
55 schema:name Two enzymes are required for strand incision in repair of alkylated DNA
56 schema:pagination 829-832
57 schema:productId N6365ad2cc7ae45ca8d85771e0e9f058e
58 Naa405d237ec047238ef86decfb0f85af
59 Necdfef916a824703af9e47bd8680db94
60 schema:sameAs https://app.dimensions.ai/details/publication/pub.1034421803
61 https://doi.org/10.1038/269829a0
62 schema:sdDatePublished 2022-05-20T07:17
63 schema:sdLicense https://scigraph.springernature.com/explorer/license/
64 schema:sdPublisher N13cbf09104974506b7ca89f78bfef6cf
65 schema:url https://doi.org/10.1038/269829a0
66 sgo:license sg:explorer/license/
67 sgo:sdDataset articles
68 rdf:type schema:ScholarlyArticle
69 N13cbf09104974506b7ca89f78bfef6cf schema:name Springer Nature - SN SciGraph project
70 rdf:type schema:Organization
71 N2192ff03972d4d99b1b5c2c7e41b059d schema:volumeNumber 269
72 rdf:type schema:PublicationVolume
73 N2368ef1de13c4968a7014b460193b43a schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
74 schema:name Apurinic Acid
75 rdf:type schema:DefinedTerm
76 N27325796273c4fd8b0e1a600a46715f8 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
77 schema:name DNA, Viral
78 rdf:type schema:DefinedTerm
79 N324b13a328974ffcb28dcf4bb28c15dc schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
80 schema:name Endonucleases
81 rdf:type schema:DefinedTerm
82 N6365ad2cc7ae45ca8d85771e0e9f058e schema:name doi
83 schema:value 10.1038/269829a0
84 rdf:type schema:PropertyValue
85 N6439ceb590a94c13b008098b96613f41 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
86 schema:name DNA
87 rdf:type schema:DefinedTerm
88 N7f94f9367ce74dca8cdc2d1f57cd710e schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
89 schema:name DNA, Superhelical
90 rdf:type schema:DefinedTerm
91 N94f579b03aa5452aa6a4c780635bfc9e schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
92 schema:name DNA, Bacterial
93 rdf:type schema:DefinedTerm
94 N9fbc7a047c194583a3d3e1fc5d6ad595 rdf:first sg:person.0710305630.49
95 rdf:rest rdf:nil
96 Naa405d237ec047238ef86decfb0f85af schema:name dimensions_id
97 schema:value pub.1034421803
98 rdf:type schema:PropertyValue
99 Nad64da4cf790401e8049b995db5176cc schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
100 schema:name DNA Repair
101 rdf:type schema:DefinedTerm
102 Nc8725c7c8f424f02af9b22c008c6dcb9 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
103 schema:name Methylation
104 rdf:type schema:DefinedTerm
105 Ne488258ee9bd4e7dbaa6f1aa8381d5d6 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
106 schema:name Glycoside Hydrolases
107 rdf:type schema:DefinedTerm
108 Necdfef916a824703af9e47bd8680db94 schema:name pubmed_id
109 schema:value 927511
110 rdf:type schema:PropertyValue
111 Nff28eab7d5664010ad485e11f0bc9b31 schema:issueNumber 5631
112 rdf:type schema:PublicationIssue
113 anzsrc-for:06 schema:inDefinedTermSet anzsrc-for:
114 schema:name Biological Sciences
115 rdf:type schema:DefinedTerm
116 anzsrc-for:0601 schema:inDefinedTermSet anzsrc-for:
117 schema:name Biochemistry and Cell Biology
118 rdf:type schema:DefinedTerm
119 sg:journal.1018957 schema:issn 0028-0836
120 1476-4687
121 schema:name Nature
122 schema:publisher Springer Nature
123 rdf:type schema:Periodical
124 sg:person.0710305630.49 schema:affiliation grid-institutes:grid.14925.3b
125 schema:familyName LAVAL
126 schema:givenName JACQUES
127 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0710305630.49
128 rdf:type schema:Person
129 sg:pub.10.1038/258544a0 schema:sameAs https://app.dimensions.ai/details/publication/pub.1008417712
130 https://doi.org/10.1038/258544a0
131 rdf:type schema:CreativeWork
132 sg:pub.10.1038/259064a0 schema:sameAs https://app.dimensions.ai/details/publication/pub.1018362577
133 https://doi.org/10.1038/259064a0
134 rdf:type schema:CreativeWork
135 grid-institutes:grid.14925.3b schema:alternateName Laboratoire Associé n 147 CNRS et Unité 147 INSERM, Institut Gustave-Roussy, 94800, Villejuif, France
136 schema:name Laboratoire Associé n 147 CNRS et Unité 147 INSERM, Institut Gustave-Roussy, 94800, Villejuif, France
137 rdf:type schema:Organization
 




Preview window. Press ESC to close (or click here)


...