Functional striated muscle cells from non-myoblast precursors following 5-azacytidine treatment View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

1977-05

AUTHORS

ILIP G. CONSTANTINIDES, PETER A. JONES, WIELAND GEVERS

ABSTRACT

5-AZACYTIDINE (aza-CR)—a s-triazine nucleoside analogue of cytidine—has strong inhibitory effects in various biological systems including neoplasms: it is a powerful bacteriostatic, antitumour and mutagenic agent1. Aza-CR also exhibits immunosuppressive, antimitotic, radioprotective and virostatic effects1. The cytostatic effect of aza-CR has primarily been directed against leukaemia2 and it has been used to treat human solid tumours3. During a study on the morphological transformation of C3H 10T½ C18 cells by cancer chemotherapeutic agents, it was noted that elongated multinucleated cells arose in cultures exposed to aza-CR4. The C3H 10T½ C18 line is a clonal line of mouse embryo cells developed at the McArdle Laboratory for Cancer Research for use in studies on chemical oncogenesis in vitro5–10. The cells are highly sensitive to post-confluence inhibition of cell division, show a remarkably low rate of spontaneous transformation and grow with a fibroblast-like morphology with long cytoplasmic processes in sparse cultures. When confluent, they form flat even monolayers and appear epithelioid. Scanning electron microscopy has shown the confluent cells to be extremely thin and polygonal with smooth surfaces resembling epithelial cells11. Here we describe experiments showing that the multinucleated tubular cells which arise in C3H 10T½ C18 cells treated with aza-CR are in fact functional myotubes. More... »

PAGES

364-366

Identifiers

URI

http://scigraph.springernature.com/pub.10.1038/267364a0

DOI

http://dx.doi.org/10.1038/267364a0

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1036920847

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/68440


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