Bacterial cloning of plasmids carrying copies of rabbit globin messenger RNA View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

1976-03

AUTHORS

T H Rabbitts

ABSTRACT

DNA copies of rabbit globin mRNA have been inserted into the plasmid mini-ColEl. After addition of poly(dT) tails, the plasmid was used to prime the copying of mRNA by reverse transcriptase. Mini-ColEl carrying globin sequences were denatured and annealed with mini-ColEl tailed with poly(dA). Functional plasmids containing the synthesised globin sequences were isolated by transfection in E. coli. More... »

PAGES

221

Identifiers

URI

http://scigraph.springernature.com/pub.10.1038/260221a0

DOI

http://dx.doi.org/10.1038/260221a0

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1020607208

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/768776


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