Half life of yeast messenger RNA View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

1976-03

AUTHORS

N. S. PETERSEN, C. S. MCLAUGHLIN, D. P. NIERLICH

ABSTRACT

WE have determined the half life of Saccharomyces cerevisiae messenger RNA (mRNA) and its associated poly (A) sequences, from the kinetics of their labelling with 14C-adenine. Corrections have been made for changes in the ATP pool specific activity during the labelling period. This method of determining the mRNA half life is preferred to observing the decay of mRNA in the presence of inhibitors because changes in the cell metabolism caused by an inhibitor may also change the mRNA half life1. One of our purposes in determining these half lives is for their use in the characterisation of temperature-sensitive mutants which are defective in macromolecular synthesis2. For this reason we have determined the half lives of mRNA and poly(A) in two different conditions, at 23 and 36 °C. More... »

PAGES

70-72

Identifiers

URI

http://scigraph.springernature.com/pub.10.1038/260070a0

DOI

http://dx.doi.org/10.1038/260070a0

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1005720315

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/772442


Indexing Status Check whether this publication has been indexed by Scopus and Web Of Science using the SN Indexing Status Tool
Incoming Citations Browse incoming citations for this publication using opencitations.net

JSON-LD is the canonical representation for SciGraph data.

TIP: You can open this SciGraph record using an external JSON-LD service: JSON-LD Playground Google SDTT

[
  {
    "@context": "https://springernature.github.io/scigraph/jsonld/sgcontext.json", 
    "about": [
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/0601", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Biochemistry and Cell Biology", 
        "type": "DefinedTerm"
      }, 
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/06", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Biological Sciences", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Adenosine Triphosphate", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Cell Division", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Guanosine Triphosphate", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Poly A", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Polyribosomes", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "RNA, Messenger", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Saccharomyces cerevisiae", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Temperature", 
        "type": "DefinedTerm"
      }
    ], 
    "author": [
      {
        "familyName": "PETERSEN", 
        "givenName": "N. S.", 
        "id": "sg:person.067722354.47", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.067722354.47"
        ], 
        "type": "Person"
      }, 
      {
        "familyName": "MCLAUGHLIN", 
        "givenName": "C. S.", 
        "id": "sg:person.0631074245.15", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0631074245.15"
        ], 
        "type": "Person"
      }, 
      {
        "familyName": "NIERLICH", 
        "givenName": "D. P.", 
        "id": "sg:person.0113725010.68", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0113725010.68"
        ], 
        "type": "Person"
      }
    ], 
    "citation": [
      {
        "id": "sg:pub.10.1007/bf00324052", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1009665960", 
          "https://doi.org/10.1007/bf00324052"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/0022-2836(67)90107-6", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1011392287"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/0022-2836(68)90151-4", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1011839227"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/240100a0", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1041163749", 
          "https://doi.org/10.1038/240100a0"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1073/pnas.62.2.468", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1044088422"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/0003-2697(65)90191-0", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1047295965"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://app.dimensions.ai/details/publication/pub.1074668211", 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://app.dimensions.ai/details/publication/pub.1080446098", 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://app.dimensions.ai/details/publication/pub.1080453984", 
        "type": "CreativeWork"
      }
    ], 
    "datePublished": "1976-03", 
    "datePublishedReg": "1976-03-01", 
    "description": "WE have determined the half life of Saccharomyces cerevisiae messenger RNA (mRNA) and its associated poly (A) sequences, from the kinetics of their labelling with 14C-adenine. Corrections have been made for changes in the ATP pool specific activity during the labelling period. This method of determining the mRNA half life is preferred to observing the decay of mRNA in the presence of inhibitors because changes in the cell metabolism caused by an inhibitor may also change the mRNA half life1. One of our purposes in determining these half lives is for their use in the characterisation of temperature-sensitive mutants which are defective in macromolecular synthesis2. For this reason we have determined the half lives of mRNA and poly(A) in two different conditions, at 23 and 36 \u00b0C.", 
    "genre": "research_article", 
    "id": "sg:pub.10.1038/260070a0", 
    "inLanguage": [
      "en"
    ], 
    "isAccessibleForFree": false, 
    "isPartOf": [
      {
        "id": "sg:journal.1018957", 
        "issn": [
          "0090-0028", 
          "1476-4687"
        ], 
        "name": "Nature", 
        "type": "Periodical"
      }, 
      {
        "issueNumber": "5546", 
        "type": "PublicationIssue"
      }, 
      {
        "type": "PublicationVolume", 
        "volumeNumber": "260"
      }
    ], 
    "name": "Half life of yeast messenger RNA", 
    "pagination": "70-72", 
    "productId": [
      {
        "name": "readcube_id", 
        "type": "PropertyValue", 
        "value": [
          "1b4119f175d98ddaa2d4288ff1236072a5cb3f6962990725d36fd130b7c830c4"
        ]
      }, 
      {
        "name": "pubmed_id", 
        "type": "PropertyValue", 
        "value": [
          "772442"
        ]
      }, 
      {
        "name": "nlm_unique_id", 
        "type": "PropertyValue", 
        "value": [
          "0410462"
        ]
      }, 
      {
        "name": "doi", 
        "type": "PropertyValue", 
        "value": [
          "10.1038/260070a0"
        ]
      }, 
      {
        "name": "dimensions_id", 
        "type": "PropertyValue", 
        "value": [
          "pub.1005720315"
        ]
      }
    ], 
    "sameAs": [
      "https://doi.org/10.1038/260070a0", 
      "https://app.dimensions.ai/details/publication/pub.1005720315"
    ], 
    "sdDataset": "articles", 
    "sdDatePublished": "2019-04-10T18:56", 
    "sdLicense": "https://scigraph.springernature.com/explorer/license/", 
    "sdPublisher": {
      "name": "Springer Nature - SN SciGraph project", 
      "type": "Organization"
    }, 
    "sdSource": "s3://com-uberresearch-data-dimensions-target-20181106-alternative/cleanup/v134/2549eaecd7973599484d7c17b260dba0a4ecb94b/merge/v9/a6c9fde33151104705d4d7ff012ea9563521a3ce/jats-lookup/v90/0000000001_0000000264/records_8678_00000421.jsonl", 
    "type": "ScholarlyArticle", 
    "url": "http://www.nature.com/articles/260070a0"
  }
]
 

Download the RDF metadata as:  json-ld nt turtle xml License info

HOW TO GET THIS DATA PROGRAMMATICALLY:

JSON-LD is a popular format for linked data which is fully compatible with JSON.

curl -H 'Accept: application/ld+json' 'https://scigraph.springernature.com/pub.10.1038/260070a0'

N-Triples is a line-based linked data format ideal for batch operations.

curl -H 'Accept: application/n-triples' 'https://scigraph.springernature.com/pub.10.1038/260070a0'

Turtle is a human-readable linked data format.

curl -H 'Accept: text/turtle' 'https://scigraph.springernature.com/pub.10.1038/260070a0'

RDF/XML is a standard XML format for linked data.

curl -H 'Accept: application/rdf+xml' 'https://scigraph.springernature.com/pub.10.1038/260070a0'


 

This table displays all metadata directly associated to this object as RDF triples.

135 TRIPLES      21 PREDICATES      46 URIs      29 LITERALS      17 BLANK NODES

Subject Predicate Object
1 sg:pub.10.1038/260070a0 schema:about N5c70e700c6e14bc2ae7050ffae116c96
2 N639a646d00b54c2885317d645ba6b2b5
3 Ncb180da22ee34dbfa4ebd907d56447ec
4 Nce2dc74c8d1246bb809806aa671ce15a
5 Nd8171b2c629641bab022a1c429c8a28c
6 Ndab68031891e4bd090ca967782f8baa4
7 Ne51953cdd72c401395e8d39c1ce675e6
8 Neae0ea7068e04d3ebe82984572e9076a
9 anzsrc-for:06
10 anzsrc-for:0601
11 schema:author Ne17cce787e5340d5ac064c7b35159813
12 schema:citation sg:pub.10.1007/bf00324052
13 sg:pub.10.1038/240100a0
14 https://app.dimensions.ai/details/publication/pub.1074668211
15 https://app.dimensions.ai/details/publication/pub.1080446098
16 https://app.dimensions.ai/details/publication/pub.1080453984
17 https://doi.org/10.1016/0003-2697(65)90191-0
18 https://doi.org/10.1016/0022-2836(67)90107-6
19 https://doi.org/10.1016/0022-2836(68)90151-4
20 https://doi.org/10.1073/pnas.62.2.468
21 schema:datePublished 1976-03
22 schema:datePublishedReg 1976-03-01
23 schema:description WE have determined the half life of Saccharomyces cerevisiae messenger RNA (mRNA) and its associated poly (A) sequences, from the kinetics of their labelling with 14C-adenine. Corrections have been made for changes in the ATP pool specific activity during the labelling period. This method of determining the mRNA half life is preferred to observing the decay of mRNA in the presence of inhibitors because changes in the cell metabolism caused by an inhibitor may also change the mRNA half life1. One of our purposes in determining these half lives is for their use in the characterisation of temperature-sensitive mutants which are defective in macromolecular synthesis2. For this reason we have determined the half lives of mRNA and poly(A) in two different conditions, at 23 and 36 °C.
24 schema:genre research_article
25 schema:inLanguage en
26 schema:isAccessibleForFree false
27 schema:isPartOf N5b34b72dc428475999342e63e2edc31e
28 Nea586c882394490ea8023580e4e164dc
29 sg:journal.1018957
30 schema:name Half life of yeast messenger RNA
31 schema:pagination 70-72
32 schema:productId N0e8dba2c244249ca9810af13fa3a7eda
33 N2d1925309ac04f4198238dff30de5d8e
34 N3069acf8545646d687bdbd0c28b64db3
35 N31804dcb9ac343288372d3951e5d9978
36 N9a604ba7648c43eda422b3729fbc053c
37 schema:sameAs https://app.dimensions.ai/details/publication/pub.1005720315
38 https://doi.org/10.1038/260070a0
39 schema:sdDatePublished 2019-04-10T18:56
40 schema:sdLicense https://scigraph.springernature.com/explorer/license/
41 schema:sdPublisher N65c7f8d57568410f9e1d6b9045d0aa0f
42 schema:url http://www.nature.com/articles/260070a0
43 sgo:license sg:explorer/license/
44 sgo:sdDataset articles
45 rdf:type schema:ScholarlyArticle
46 N0e8dba2c244249ca9810af13fa3a7eda schema:name doi
47 schema:value 10.1038/260070a0
48 rdf:type schema:PropertyValue
49 N2d1925309ac04f4198238dff30de5d8e schema:name pubmed_id
50 schema:value 772442
51 rdf:type schema:PropertyValue
52 N3069acf8545646d687bdbd0c28b64db3 schema:name nlm_unique_id
53 schema:value 0410462
54 rdf:type schema:PropertyValue
55 N3099b358230c4c8194f68c3917d07b86 rdf:first sg:person.0113725010.68
56 rdf:rest rdf:nil
57 N31804dcb9ac343288372d3951e5d9978 schema:name readcube_id
58 schema:value 1b4119f175d98ddaa2d4288ff1236072a5cb3f6962990725d36fd130b7c830c4
59 rdf:type schema:PropertyValue
60 N5b34b72dc428475999342e63e2edc31e schema:volumeNumber 260
61 rdf:type schema:PublicationVolume
62 N5c70e700c6e14bc2ae7050ffae116c96 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
63 schema:name Temperature
64 rdf:type schema:DefinedTerm
65 N5d3ef6da82f643d38a43b0368311aa86 rdf:first sg:person.0631074245.15
66 rdf:rest N3099b358230c4c8194f68c3917d07b86
67 N639a646d00b54c2885317d645ba6b2b5 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
68 schema:name Polyribosomes
69 rdf:type schema:DefinedTerm
70 N65c7f8d57568410f9e1d6b9045d0aa0f schema:name Springer Nature - SN SciGraph project
71 rdf:type schema:Organization
72 N9a604ba7648c43eda422b3729fbc053c schema:name dimensions_id
73 schema:value pub.1005720315
74 rdf:type schema:PropertyValue
75 Ncb180da22ee34dbfa4ebd907d56447ec schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
76 schema:name Adenosine Triphosphate
77 rdf:type schema:DefinedTerm
78 Nce2dc74c8d1246bb809806aa671ce15a schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
79 schema:name RNA, Messenger
80 rdf:type schema:DefinedTerm
81 Nd8171b2c629641bab022a1c429c8a28c schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
82 schema:name Saccharomyces cerevisiae
83 rdf:type schema:DefinedTerm
84 Ndab68031891e4bd090ca967782f8baa4 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
85 schema:name Poly A
86 rdf:type schema:DefinedTerm
87 Ne17cce787e5340d5ac064c7b35159813 rdf:first sg:person.067722354.47
88 rdf:rest N5d3ef6da82f643d38a43b0368311aa86
89 Ne51953cdd72c401395e8d39c1ce675e6 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
90 schema:name Cell Division
91 rdf:type schema:DefinedTerm
92 Nea586c882394490ea8023580e4e164dc schema:issueNumber 5546
93 rdf:type schema:PublicationIssue
94 Neae0ea7068e04d3ebe82984572e9076a schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
95 schema:name Guanosine Triphosphate
96 rdf:type schema:DefinedTerm
97 anzsrc-for:06 schema:inDefinedTermSet anzsrc-for:
98 schema:name Biological Sciences
99 rdf:type schema:DefinedTerm
100 anzsrc-for:0601 schema:inDefinedTermSet anzsrc-for:
101 schema:name Biochemistry and Cell Biology
102 rdf:type schema:DefinedTerm
103 sg:journal.1018957 schema:issn 0090-0028
104 1476-4687
105 schema:name Nature
106 rdf:type schema:Periodical
107 sg:person.0113725010.68 schema:familyName NIERLICH
108 schema:givenName D. P.
109 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0113725010.68
110 rdf:type schema:Person
111 sg:person.0631074245.15 schema:familyName MCLAUGHLIN
112 schema:givenName C. S.
113 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0631074245.15
114 rdf:type schema:Person
115 sg:person.067722354.47 schema:familyName PETERSEN
116 schema:givenName N. S.
117 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.067722354.47
118 rdf:type schema:Person
119 sg:pub.10.1007/bf00324052 schema:sameAs https://app.dimensions.ai/details/publication/pub.1009665960
120 https://doi.org/10.1007/bf00324052
121 rdf:type schema:CreativeWork
122 sg:pub.10.1038/240100a0 schema:sameAs https://app.dimensions.ai/details/publication/pub.1041163749
123 https://doi.org/10.1038/240100a0
124 rdf:type schema:CreativeWork
125 https://app.dimensions.ai/details/publication/pub.1074668211 schema:CreativeWork
126 https://app.dimensions.ai/details/publication/pub.1080446098 schema:CreativeWork
127 https://app.dimensions.ai/details/publication/pub.1080453984 schema:CreativeWork
128 https://doi.org/10.1016/0003-2697(65)90191-0 schema:sameAs https://app.dimensions.ai/details/publication/pub.1047295965
129 rdf:type schema:CreativeWork
130 https://doi.org/10.1016/0022-2836(67)90107-6 schema:sameAs https://app.dimensions.ai/details/publication/pub.1011392287
131 rdf:type schema:CreativeWork
132 https://doi.org/10.1016/0022-2836(68)90151-4 schema:sameAs https://app.dimensions.ai/details/publication/pub.1011839227
133 rdf:type schema:CreativeWork
134 https://doi.org/10.1073/pnas.62.2.468 schema:sameAs https://app.dimensions.ai/details/publication/pub.1044088422
135 rdf:type schema:CreativeWork
 




Preview window. Press ESC to close (or click here)


...