Ontology type: schema:ScholarlyArticle
1975-08
AUTHORS ABSTRACTTHE manufacture of predefined specific antibodies by means of permanent tissue culture cell lines is of general interest. There are at present a considerable number of permanent cultures of myeloma cells1,2 and screening procedures have been used to reveal antibody activity in some of them. This, however, is not a satisfactory source of monoclonal antibodies of predefined specificity. We describe here the derivation of a number of tissue culture cell lines which secrete anti-sheep red blood cell (SRBC) antibodies. The cell lines are made by fusion of a mouse myeloma and mouse spleen cells from an immunised donor. To understand the expression and interactions of the Ig chains from the parental lines, fusion experiments between two known mouse myeloma lines were carried out. More... »
PAGES495-497
http://scigraph.springernature.com/pub.10.1038/256495a0
DOIhttp://dx.doi.org/10.1038/256495a0
DIMENSIONShttps://app.dimensions.ai/details/publication/pub.1001387921
PUBMEDhttps://www.ncbi.nlm.nih.gov/pubmed/1172191
JSON-LD is the canonical representation for SciGraph data.
TIP: You can open this SciGraph record using an external JSON-LD service: JSON-LD Playground Google SDTT
[
{
"@context": "https://springernature.github.io/scigraph/jsonld/sgcontext.json",
"about": [
{
"id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/11",
"inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/",
"name": "Medical and Health Sciences",
"type": "DefinedTerm"
},
{
"id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/1107",
"inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/",
"name": "Immunology",
"type": "DefinedTerm"
},
{
"inDefinedTermSet": "https://www.nlm.nih.gov/mesh/",
"name": "Animals",
"type": "DefinedTerm"
},
{
"inDefinedTermSet": "https://www.nlm.nih.gov/mesh/",
"name": "Antibody Formation",
"type": "DefinedTerm"
},
{
"inDefinedTermSet": "https://www.nlm.nih.gov/mesh/",
"name": "Antibody Specificity",
"type": "DefinedTerm"
},
{
"inDefinedTermSet": "https://www.nlm.nih.gov/mesh/",
"name": "Cell Fusion",
"type": "DefinedTerm"
},
{
"inDefinedTermSet": "https://www.nlm.nih.gov/mesh/",
"name": "Cell Line",
"type": "DefinedTerm"
},
{
"inDefinedTermSet": "https://www.nlm.nih.gov/mesh/",
"name": "Culture Techniques",
"type": "DefinedTerm"
},
{
"inDefinedTermSet": "https://www.nlm.nih.gov/mesh/",
"name": "Erythrocytes",
"type": "DefinedTerm"
},
{
"inDefinedTermSet": "https://www.nlm.nih.gov/mesh/",
"name": "Genetic Code",
"type": "DefinedTerm"
},
{
"inDefinedTermSet": "https://www.nlm.nih.gov/mesh/",
"name": "Immunoglobulins",
"type": "DefinedTerm"
},
{
"inDefinedTermSet": "https://www.nlm.nih.gov/mesh/",
"name": "Mice",
"type": "DefinedTerm"
},
{
"inDefinedTermSet": "https://www.nlm.nih.gov/mesh/",
"name": "Mice, Inbred BALB C",
"type": "DefinedTerm"
},
{
"inDefinedTermSet": "https://www.nlm.nih.gov/mesh/",
"name": "Multiple Myeloma",
"type": "DefinedTerm"
},
{
"inDefinedTermSet": "https://www.nlm.nih.gov/mesh/",
"name": "Spleen",
"type": "DefinedTerm"
}
],
"author": [
{
"affiliation": {
"alternateName": "MRC Laboratory of Molecular Biology, Hills Road, CB2 2QH, Cambridge, UK",
"id": "http://www.grid.ac/institutes/grid.42475.30",
"name": [
"MRC Laboratory of Molecular Biology, Hills Road, CB2 2QH, Cambridge, UK"
],
"type": "Organization"
},
"familyName": "K\u00d6HLER",
"givenName": "G.",
"id": "sg:person.016454164452.97",
"sameAs": [
"https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.016454164452.97"
],
"type": "Person"
},
{
"affiliation": {
"alternateName": "MRC Laboratory of Molecular Biology, Hills Road, CB2 2QH, Cambridge, UK",
"id": "http://www.grid.ac/institutes/grid.42475.30",
"name": [
"MRC Laboratory of Molecular Biology, Hills Road, CB2 2QH, Cambridge, UK"
],
"type": "Organization"
},
"familyName": "MILSTEIN",
"givenName": "C.",
"id": "sg:person.0104563066.64",
"sameAs": [
"https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0104563066.64"
],
"type": "Person"
}
],
"citation": [
{
"id": "sg:pub.10.1038/252354a0",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1020752516",
"https://doi.org/10.1038/252354a0"
],
"type": "CreativeWork"
},
{
"id": "sg:pub.10.1038/219066a0",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1021244471",
"https://doi.org/10.1038/219066a0"
],
"type": "CreativeWork"
},
{
"id": "sg:pub.10.1038/205640a0",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1001120649",
"https://doi.org/10.1038/205640a0"
],
"type": "CreativeWork"
},
{
"id": "sg:pub.10.1038/244042a0",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1004074762",
"https://doi.org/10.1038/244042a0"
],
"type": "CreativeWork"
},
{
"id": "sg:pub.10.1038/224597a0",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1026266151",
"https://doi.org/10.1038/224597a0"
],
"type": "CreativeWork"
}
],
"datePublished": "1975-08",
"datePublishedReg": "1975-08-01",
"description": "THE manufacture of predefined specific antibodies by means of permanent tissue culture cell lines is of general interest. There are at present a considerable number of permanent cultures of myeloma cells1,2 and screening procedures have been used to reveal antibody activity in some of them. This, however, is not a satisfactory source of monoclonal antibodies of predefined specificity. We describe here the derivation of a number of tissue culture cell lines which secrete anti-sheep red blood cell (SRBC) antibodies. The cell lines are made by fusion of a mouse myeloma and mouse spleen cells from an immunised donor. To understand the expression and interactions of the Ig chains from the parental lines, fusion experiments between two known mouse myeloma lines were carried out.",
"genre": "article",
"id": "sg:pub.10.1038/256495a0",
"isAccessibleForFree": false,
"isPartOf": [
{
"id": "sg:journal.1018957",
"issn": [
"0028-0836",
"1476-4687"
],
"name": "Nature",
"publisher": "Springer Nature",
"type": "Periodical"
},
{
"issueNumber": "5517",
"type": "PublicationIssue"
},
{
"type": "PublicationVolume",
"volumeNumber": "256"
}
],
"keywords": [
"tissue culture cell lines",
"red blood cell antibodies",
"culture cell lines",
"cell lines",
"anti-sheep red blood cell antibody",
"mouse spleen cells",
"mouse myeloma line",
"cell antibodies",
"spleen cells",
"antibody activity",
"monoclonal antibodies",
"specific antibodies",
"antibodies",
"myeloma line",
"myeloma",
"mouse myeloma",
"fused cells",
"cells",
"specificity",
"Ig chains",
"considerable number",
"permanent cultures",
"lines",
"culture",
"donors",
"expression",
"activity",
"number",
"procedure",
"satisfactory source",
"fusion",
"parental lines",
"means",
"continuous culture",
"interest",
"interaction",
"source",
"general interest",
"experiments",
"chain",
"fusion experiments",
"manufacture",
"derivation"
],
"name": "Continuous cultures of fused cells secreting antibody of predefined specificity",
"pagination": "495-497",
"productId": [
{
"name": "dimensions_id",
"type": "PropertyValue",
"value": [
"pub.1001387921"
]
},
{
"name": "doi",
"type": "PropertyValue",
"value": [
"10.1038/256495a0"
]
},
{
"name": "pubmed_id",
"type": "PropertyValue",
"value": [
"1172191"
]
}
],
"sameAs": [
"https://doi.org/10.1038/256495a0",
"https://app.dimensions.ai/details/publication/pub.1001387921"
],
"sdDataset": "articles",
"sdDatePublished": "2022-11-24T20:45",
"sdLicense": "https://scigraph.springernature.com/explorer/license/",
"sdPublisher": {
"name": "Springer Nature - SN SciGraph project",
"type": "Organization"
},
"sdSource": "s3://com-springernature-scigraph/baseset/20221124/entities/gbq_results/article/article_145.jsonl",
"type": "ScholarlyArticle",
"url": "https://doi.org/10.1038/256495a0"
}
]Download the RDF metadata as: json-ld nt turtle xml License info
JSON-LD is a popular format for linked data which is fully compatible with JSON.
curl -H 'Accept: application/ld+json' 'https://scigraph.springernature.com/pub.10.1038/256495a0'
N-Triples is a line-based linked data format ideal for batch operations.
curl -H 'Accept: application/n-triples' 'https://scigraph.springernature.com/pub.10.1038/256495a0'
Turtle is a human-readable linked data format.
curl -H 'Accept: text/turtle' 'https://scigraph.springernature.com/pub.10.1038/256495a0'
RDF/XML is a standard XML format for linked data.
curl -H 'Accept: application/rdf+xml' 'https://scigraph.springernature.com/pub.10.1038/256495a0'
This table displays all metadata directly associated to this object as RDF triples.
183 TRIPLES
21 PREDICATES
87 URIs
74 LITERALS
20 BLANK NODES
| Subject | Predicate | Object | |
|---|---|---|---|
| 1 | sg:pub.10.1038/256495a0 | schema:about | N2114d9bb0451482d9bccd8fd3038c6c9 |
| 2 | ″ | ″ | N385919e5ef5b44499746b5237e99134c |
| 3 | ″ | ″ | N5c7ba4056efb40c29c5e8420431bc099 |
| 4 | ″ | ″ | N5f802937d0164529b43eda461ef9386c |
| 5 | ″ | ″ | N64896d2d383d4943a30ec4c1eddf8515 |
| 6 | ″ | ″ | N6dfdb4b6d2ab41ecba70062045d09225 |
| 7 | ″ | ″ | N72c3348f9aad4c849012f3d7a08c7220 |
| 8 | ″ | ″ | N8d4c585b47664c078f32df8e124814da |
| 9 | ″ | ″ | Na20933e0f88a4353ba9c7b92d081588f |
| 10 | ″ | ″ | Nd02e169781c748efaf92ee8e85c7aacb |
| 11 | ″ | ″ | Ne15a430c50544691b372b33901b4ec6a |
| 12 | ″ | ″ | Nf308e2057b324ecd808e23f1478d0785 |
| 13 | ″ | ″ | Nfdc955ac3df84b52bc09afa2abb0ed08 |
| 14 | ″ | ″ | anzsrc-for:11 |
| 15 | ″ | ″ | anzsrc-for:1107 |
| 16 | ″ | schema:author | Ne45aaabe7513445db580d761cdb75c7d |
| 17 | ″ | schema:citation | sg:pub.10.1038/205640a0 |
| 18 | ″ | ″ | sg:pub.10.1038/219066a0 |
| 19 | ″ | ″ | sg:pub.10.1038/224597a0 |
| 20 | ″ | ″ | sg:pub.10.1038/244042a0 |
| 21 | ″ | ″ | sg:pub.10.1038/252354a0 |
| 22 | ″ | schema:datePublished | 1975-08 |
| 23 | ″ | schema:datePublishedReg | 1975-08-01 |
| 24 | ″ | schema:description | THE manufacture of predefined specific antibodies by means of permanent tissue culture cell lines is of general interest. There are at present a considerable number of permanent cultures of myeloma cells1,2 and screening procedures have been used to reveal antibody activity in some of them. This, however, is not a satisfactory source of monoclonal antibodies of predefined specificity. We describe here the derivation of a number of tissue culture cell lines which secrete anti-sheep red blood cell (SRBC) antibodies. The cell lines are made by fusion of a mouse myeloma and mouse spleen cells from an immunised donor. To understand the expression and interactions of the Ig chains from the parental lines, fusion experiments between two known mouse myeloma lines were carried out. |
| 25 | ″ | schema:genre | article |
| 26 | ″ | schema:isAccessibleForFree | false |
| 27 | ″ | schema:isPartOf | N2f65d74c82cd4516ae39ed937d83f407 |
| 28 | ″ | ″ | N3559541335214c42963b69d022d244ad |
| 29 | ″ | ″ | sg:journal.1018957 |
| 30 | ″ | schema:keywords | Ig chains |
| 31 | ″ | ″ | activity |
| 32 | ″ | ″ | anti-sheep red blood cell antibody |
| 33 | ″ | ″ | antibodies |
| 34 | ″ | ″ | antibody activity |
| 35 | ″ | ″ | cell antibodies |
| 36 | ″ | ″ | cell lines |
| 37 | ″ | ″ | cells |
| 38 | ″ | ″ | chain |
| 39 | ″ | ″ | considerable number |
| 40 | ″ | ″ | continuous culture |
| 41 | ″ | ″ | culture |
| 42 | ″ | ″ | culture cell lines |
| 43 | ″ | ″ | derivation |
| 44 | ″ | ″ | donors |
| 45 | ″ | ″ | experiments |
| 46 | ″ | ″ | expression |
| 47 | ″ | ″ | fused cells |
| 48 | ″ | ″ | fusion |
| 49 | ″ | ″ | fusion experiments |
| 50 | ″ | ″ | general interest |
| 51 | ″ | ″ | interaction |
| 52 | ″ | ″ | interest |
| 53 | ″ | ″ | lines |
| 54 | ″ | ″ | manufacture |
| 55 | ″ | ″ | means |
| 56 | ″ | ″ | monoclonal antibodies |
| 57 | ″ | ″ | mouse myeloma |
| 58 | ″ | ″ | mouse myeloma line |
| 59 | ″ | ″ | mouse spleen cells |
| 60 | ″ | ″ | myeloma |
| 61 | ″ | ″ | myeloma line |
| 62 | ″ | ″ | number |
| 63 | ″ | ″ | parental lines |
| 64 | ″ | ″ | permanent cultures |
| 65 | ″ | ″ | procedure |
| 66 | ″ | ″ | red blood cell antibodies |
| 67 | ″ | ″ | satisfactory source |
| 68 | ″ | ″ | source |
| 69 | ″ | ″ | specific antibodies |
| 70 | ″ | ″ | specificity |
| 71 | ″ | ″ | spleen cells |
| 72 | ″ | ″ | tissue culture cell lines |
| 73 | ″ | schema:name | Continuous cultures of fused cells secreting antibody of predefined specificity |
| 74 | ″ | schema:pagination | 495-497 |
| 75 | ″ | schema:productId | N06e2b50035394748964084638c761206 |
| 76 | ″ | ″ | N24c42627b5034c49b32c7b991b36144f |
| 77 | ″ | ″ | N5af1e900b4de4e8e96bcb3d914d333dc |
| 78 | ″ | schema:sameAs | https://app.dimensions.ai/details/publication/pub.1001387921 |
| 79 | ″ | ″ | https://doi.org/10.1038/256495a0 |
| 80 | ″ | schema:sdDatePublished | 2022-11-24T20:45 |
| 81 | ″ | schema:sdLicense | https://scigraph.springernature.com/explorer/license/ |
| 82 | ″ | schema:sdPublisher | Nc3713afc9de948d0818c63e3259f3379 |
| 83 | ″ | schema:url | https://doi.org/10.1038/256495a0 |
| 84 | ″ | sgo:license | sg:explorer/license/ |
| 85 | ″ | sgo:sdDataset | articles |
| 86 | ″ | rdf:type | schema:ScholarlyArticle |
| 87 | N06e2b50035394748964084638c761206 | schema:name | doi |
| 88 | ″ | schema:value | 10.1038/256495a0 |
| 89 | ″ | rdf:type | schema:PropertyValue |
| 90 | N19419bd4eabb4be6af75808d68cef082 | rdf:first | sg:person.0104563066.64 |
| 91 | ″ | rdf:rest | rdf:nil |
| 92 | N2114d9bb0451482d9bccd8fd3038c6c9 | schema:inDefinedTermSet | https://www.nlm.nih.gov/mesh/ |