Partially single-stranded form of free Moloney viral DNA View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

1975-06

AUTHORS

ALESSANDRO M. GIANNI, ROBERT A. WEINBERG

ABSTRACT

SEVERAL hours after infection of cells by RNA tumour viruses new, free viral DNA molecules can be detected1–5 which are apparently intermediate between the initial RNA-dependent DNA transcription product and the final integrated viral genome1,6. Work on Rous sarcoma virus6,7 and Moloney murine leukaemia virus (Mo-MuLV)4 showed that this DNA is resolvable by ethidium bromide–caesium chloride (EB–CsCl) centrifugation into two classes of molecule. The first, from the lower band of the EB–CsCl gradient, contains closed-circular, double-stranded, super-coiled viral DNA molecules of molecular weight 5×106–6×106, about twice the weight of the single-stranded subunits isolated from virion RNA. The kinetics of disappearance of these DNA molecules suggest that they are the precursors of viral genomes integrated into cellular DNA5. The second class of viral DNA, from the upper band of the gradient, is characterised here. These molecules have the properties of intermediates between the initially synthesised RNA–DNA hybrids and the double-stranded, supercoiled form of the genome. Careful preparation reveals that these molecules are partially single stranded. The first strand of the viral DNA is complexed with shorter segments of the subsequently synthesised second strand. More... »

PAGES

646

Identifiers

URI

http://scigraph.springernature.com/pub.10.1038/255646a0

DOI

http://dx.doi.org/10.1038/255646a0

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1048893564

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/1134557


Indexing Status Check whether this publication has been indexed by Scopus and Web Of Science using the SN Indexing Status Tool
Incoming Citations Browse incoming citations for this publication using opencitations.net

JSON-LD is the canonical representation for SciGraph data.

TIP: You can open this SciGraph record using an external JSON-LD service: JSON-LD Playground Google SDTT

[
  {
    "@context": "https://springernature.github.io/scigraph/jsonld/sgcontext.json", 
    "about": [
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/0604", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Genetics", 
        "type": "DefinedTerm"
      }, 
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/06", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Biological Sciences", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Centrifugation, Isopycnic", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "DNA, Single-Stranded", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "DNA, Viral", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Moloney murine leukemia virus", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Nucleic Acid Denaturation", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Nucleic Acid Hybridization", 
        "type": "DefinedTerm"
      }
    ], 
    "author": [
      {
        "familyName": "GIANNI", 
        "givenName": "ALESSANDRO M.", 
        "id": "sg:person.01074017453.06", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01074017453.06"
        ], 
        "type": "Person"
      }, 
      {
        "familyName": "WEINBERG", 
        "givenName": "ROBERT A.", 
        "id": "sg:person.01224550056.38", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01224550056.38"
        ], 
        "type": "Person"
      }
    ], 
    "citation": [
      {
        "id": "https://doi.org/10.1073/pnas.71.10.3874", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1013727357"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/0022-2836(73)90235-0", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1019804747"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1073/pnas.70.11.3067", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1022031124"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/0042-6822(70)90362-4", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1029579001"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/0022-2836(67)90307-5", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1035062164"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/253507a0", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1040295853", 
          "https://doi.org/10.1038/253507a0"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1073/pnas.72.2.447", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1044786319"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/0042-6822(74)90323-7", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1049862702"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1021/bi00747a020", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1055187273"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1101/sqb.1974.039.01.113", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1060404506"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://app.dimensions.ai/details/publication/pub.1080298329", 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://app.dimensions.ai/details/publication/pub.1081158672", 
        "type": "CreativeWork"
      }
    ], 
    "datePublished": "1975-06", 
    "datePublishedReg": "1975-06-01", 
    "description": "SEVERAL hours after infection of cells by RNA tumour viruses new, free viral DNA molecules can be detected1\u20135 which are apparently intermediate between the initial RNA-dependent DNA transcription product and the final integrated viral genome1,6. Work on Rous sarcoma virus6,7 and Moloney murine leukaemia virus (Mo-MuLV)4 showed that this DNA is resolvable by ethidium bromide\u2013caesium chloride (EB\u2013CsCl) centrifugation into two classes of molecule. The first, from the lower band of the EB\u2013CsCl gradient, contains closed-circular, double-stranded, super-coiled viral DNA molecules of molecular weight 5\u00d7106\u20136\u00d7106, about twice the weight of the single-stranded subunits isolated from virion RNA. The kinetics of disappearance of these DNA molecules suggest that they are the precursors of viral genomes integrated into cellular DNA5. The second class of viral DNA, from the upper band of the gradient, is characterised here. These molecules have the properties of intermediates between the initially synthesised RNA\u2013DNA hybrids and the double-stranded, supercoiled form of the genome. Careful preparation reveals that these molecules are partially single stranded. The first strand of the viral DNA is complexed with shorter segments of the subsequently synthesised second strand.", 
    "genre": "research_article", 
    "id": "sg:pub.10.1038/255646a0", 
    "inLanguage": [
      "en"
    ], 
    "isAccessibleForFree": false, 
    "isPartOf": [
      {
        "id": "sg:journal.1018957", 
        "issn": [
          "0090-0028", 
          "1476-4687"
        ], 
        "name": "Nature", 
        "type": "Periodical"
      }, 
      {
        "issueNumber": "5510", 
        "type": "PublicationIssue"
      }, 
      {
        "type": "PublicationVolume", 
        "volumeNumber": "255"
      }
    ], 
    "name": "Partially single-stranded form of free Moloney viral DNA", 
    "pagination": "646", 
    "productId": [
      {
        "name": "readcube_id", 
        "type": "PropertyValue", 
        "value": [
          "e8eb548ca893db5aa681175d063e7df380e80e7c84e0241566daea64bc49b5d7"
        ]
      }, 
      {
        "name": "pubmed_id", 
        "type": "PropertyValue", 
        "value": [
          "1134557"
        ]
      }, 
      {
        "name": "nlm_unique_id", 
        "type": "PropertyValue", 
        "value": [
          "0410462"
        ]
      }, 
      {
        "name": "doi", 
        "type": "PropertyValue", 
        "value": [
          "10.1038/255646a0"
        ]
      }, 
      {
        "name": "dimensions_id", 
        "type": "PropertyValue", 
        "value": [
          "pub.1048893564"
        ]
      }
    ], 
    "sameAs": [
      "https://doi.org/10.1038/255646a0", 
      "https://app.dimensions.ai/details/publication/pub.1048893564"
    ], 
    "sdDataset": "articles", 
    "sdDatePublished": "2019-04-10T20:35", 
    "sdLicense": "https://scigraph.springernature.com/explorer/license/", 
    "sdPublisher": {
      "name": "Springer Nature - SN SciGraph project", 
      "type": "Organization"
    }, 
    "sdSource": "s3://com-uberresearch-data-dimensions-target-20181106-alternative/cleanup/v134/2549eaecd7973599484d7c17b260dba0a4ecb94b/merge/v9/a6c9fde33151104705d4d7ff012ea9563521a3ce/jats-lookup/v90/0000000001_0000000264/records_8684_00000426.jsonl", 
    "type": "ScholarlyArticle", 
    "url": "https://www.nature.com/articles/255646a0"
  }
]
 

Download the RDF metadata as:  json-ld nt turtle xml License info

HOW TO GET THIS DATA PROGRAMMATICALLY:

JSON-LD is a popular format for linked data which is fully compatible with JSON.

curl -H 'Accept: application/ld+json' 'https://scigraph.springernature.com/pub.10.1038/255646a0'

N-Triples is a line-based linked data format ideal for batch operations.

curl -H 'Accept: application/n-triples' 'https://scigraph.springernature.com/pub.10.1038/255646a0'

Turtle is a human-readable linked data format.

curl -H 'Accept: text/turtle' 'https://scigraph.springernature.com/pub.10.1038/255646a0'

RDF/XML is a standard XML format for linked data.

curl -H 'Accept: application/rdf+xml' 'https://scigraph.springernature.com/pub.10.1038/255646a0'


 

This table displays all metadata directly associated to this object as RDF triples.

130 TRIPLES      21 PREDICATES      47 URIs      27 LITERALS      15 BLANK NODES

Subject Predicate Object
1 sg:pub.10.1038/255646a0 schema:about N03ef64edd6d24e889c755864825b9fdd
2 N3f59783cfbe4474f8a6a95306b964b50
3 N9366566c6c534deb8e7302e4cf381fb2
4 Ncde9523d972a429786e3138c9c0c4c4a
5 Nddfaf784c69549ab99df570953ae154e
6 Ne00a8971aaee4323b64bf77e42cfd127
7 anzsrc-for:06
8 anzsrc-for:0604
9 schema:author N8052452ff53446c08d289fa0073bbf22
10 schema:citation sg:pub.10.1038/253507a0
11 https://app.dimensions.ai/details/publication/pub.1080298329
12 https://app.dimensions.ai/details/publication/pub.1081158672
13 https://doi.org/10.1016/0022-2836(67)90307-5
14 https://doi.org/10.1016/0022-2836(73)90235-0
15 https://doi.org/10.1016/0042-6822(70)90362-4
16 https://doi.org/10.1016/0042-6822(74)90323-7
17 https://doi.org/10.1021/bi00747a020
18 https://doi.org/10.1073/pnas.70.11.3067
19 https://doi.org/10.1073/pnas.71.10.3874
20 https://doi.org/10.1073/pnas.72.2.447
21 https://doi.org/10.1101/sqb.1974.039.01.113
22 schema:datePublished 1975-06
23 schema:datePublishedReg 1975-06-01
24 schema:description SEVERAL hours after infection of cells by RNA tumour viruses new, free viral DNA molecules can be detected1–5 which are apparently intermediate between the initial RNA-dependent DNA transcription product and the final integrated viral genome1,6. Work on Rous sarcoma virus6,7 and Moloney murine leukaemia virus (Mo-MuLV)4 showed that this DNA is resolvable by ethidium bromide–caesium chloride (EB–CsCl) centrifugation into two classes of molecule. The first, from the lower band of the EB–CsCl gradient, contains closed-circular, double-stranded, super-coiled viral DNA molecules of molecular weight 5×106–6×106, about twice the weight of the single-stranded subunits isolated from virion RNA. The kinetics of disappearance of these DNA molecules suggest that they are the precursors of viral genomes integrated into cellular DNA5. The second class of viral DNA, from the upper band of the gradient, is characterised here. These molecules have the properties of intermediates between the initially synthesised RNA–DNA hybrids and the double-stranded, supercoiled form of the genome. Careful preparation reveals that these molecules are partially single stranded. The first strand of the viral DNA is complexed with shorter segments of the subsequently synthesised second strand.
25 schema:genre research_article
26 schema:inLanguage en
27 schema:isAccessibleForFree false
28 schema:isPartOf N67056e11a87c4feead12a88f38ca931a
29 Nc12d2e2b8c304a15b0af787a273ec33d
30 sg:journal.1018957
31 schema:name Partially single-stranded form of free Moloney viral DNA
32 schema:pagination 646
33 schema:productId N17141d43ba6940dabf9c51810d393338
34 N7fd7e07df81d4b359875a28b3537a95e
35 Naca9802391094e2487469ed7648ea36c
36 Nfc346ae7b8884e24bcfca3fd2fdd1dd0
37 Nff3935eed64f4332ab8f49169fbdb373
38 schema:sameAs https://app.dimensions.ai/details/publication/pub.1048893564
39 https://doi.org/10.1038/255646a0
40 schema:sdDatePublished 2019-04-10T20:35
41 schema:sdLicense https://scigraph.springernature.com/explorer/license/
42 schema:sdPublisher Nec7f27b7cfd64bfc9a2d35c8acc95ede
43 schema:url https://www.nature.com/articles/255646a0
44 sgo:license sg:explorer/license/
45 sgo:sdDataset articles
46 rdf:type schema:ScholarlyArticle
47 N03ef64edd6d24e889c755864825b9fdd schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
48 schema:name Nucleic Acid Denaturation
49 rdf:type schema:DefinedTerm
50 N17141d43ba6940dabf9c51810d393338 schema:name pubmed_id
51 schema:value 1134557
52 rdf:type schema:PropertyValue
53 N3f59783cfbe4474f8a6a95306b964b50 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
54 schema:name Moloney murine leukemia virus
55 rdf:type schema:DefinedTerm
56 N67056e11a87c4feead12a88f38ca931a schema:issueNumber 5510
57 rdf:type schema:PublicationIssue
58 N7fd7e07df81d4b359875a28b3537a95e schema:name readcube_id
59 schema:value e8eb548ca893db5aa681175d063e7df380e80e7c84e0241566daea64bc49b5d7
60 rdf:type schema:PropertyValue
61 N8052452ff53446c08d289fa0073bbf22 rdf:first sg:person.01074017453.06
62 rdf:rest Nba80fdea4f894b7281d95c26aaf6af3e
63 N9366566c6c534deb8e7302e4cf381fb2 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
64 schema:name DNA, Viral
65 rdf:type schema:DefinedTerm
66 Naca9802391094e2487469ed7648ea36c schema:name doi
67 schema:value 10.1038/255646a0
68 rdf:type schema:PropertyValue
69 Nba80fdea4f894b7281d95c26aaf6af3e rdf:first sg:person.01224550056.38
70 rdf:rest rdf:nil
71 Nc12d2e2b8c304a15b0af787a273ec33d schema:volumeNumber 255
72 rdf:type schema:PublicationVolume
73 Ncde9523d972a429786e3138c9c0c4c4a schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
74 schema:name Centrifugation, Isopycnic
75 rdf:type schema:DefinedTerm
76 Nddfaf784c69549ab99df570953ae154e schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
77 schema:name DNA, Single-Stranded
78 rdf:type schema:DefinedTerm
79 Ne00a8971aaee4323b64bf77e42cfd127 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
80 schema:name Nucleic Acid Hybridization
81 rdf:type schema:DefinedTerm
82 Nec7f27b7cfd64bfc9a2d35c8acc95ede schema:name Springer Nature - SN SciGraph project
83 rdf:type schema:Organization
84 Nfc346ae7b8884e24bcfca3fd2fdd1dd0 schema:name dimensions_id
85 schema:value pub.1048893564
86 rdf:type schema:PropertyValue
87 Nff3935eed64f4332ab8f49169fbdb373 schema:name nlm_unique_id
88 schema:value 0410462
89 rdf:type schema:PropertyValue
90 anzsrc-for:06 schema:inDefinedTermSet anzsrc-for:
91 schema:name Biological Sciences
92 rdf:type schema:DefinedTerm
93 anzsrc-for:0604 schema:inDefinedTermSet anzsrc-for:
94 schema:name Genetics
95 rdf:type schema:DefinedTerm
96 sg:journal.1018957 schema:issn 0090-0028
97 1476-4687
98 schema:name Nature
99 rdf:type schema:Periodical
100 sg:person.01074017453.06 schema:familyName GIANNI
101 schema:givenName ALESSANDRO M.
102 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01074017453.06
103 rdf:type schema:Person
104 sg:person.01224550056.38 schema:familyName WEINBERG
105 schema:givenName ROBERT A.
106 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01224550056.38
107 rdf:type schema:Person
108 sg:pub.10.1038/253507a0 schema:sameAs https://app.dimensions.ai/details/publication/pub.1040295853
109 https://doi.org/10.1038/253507a0
110 rdf:type schema:CreativeWork
111 https://app.dimensions.ai/details/publication/pub.1080298329 schema:CreativeWork
112 https://app.dimensions.ai/details/publication/pub.1081158672 schema:CreativeWork
113 https://doi.org/10.1016/0022-2836(67)90307-5 schema:sameAs https://app.dimensions.ai/details/publication/pub.1035062164
114 rdf:type schema:CreativeWork
115 https://doi.org/10.1016/0022-2836(73)90235-0 schema:sameAs https://app.dimensions.ai/details/publication/pub.1019804747
116 rdf:type schema:CreativeWork
117 https://doi.org/10.1016/0042-6822(70)90362-4 schema:sameAs https://app.dimensions.ai/details/publication/pub.1029579001
118 rdf:type schema:CreativeWork
119 https://doi.org/10.1016/0042-6822(74)90323-7 schema:sameAs https://app.dimensions.ai/details/publication/pub.1049862702
120 rdf:type schema:CreativeWork
121 https://doi.org/10.1021/bi00747a020 schema:sameAs https://app.dimensions.ai/details/publication/pub.1055187273
122 rdf:type schema:CreativeWork
123 https://doi.org/10.1073/pnas.70.11.3067 schema:sameAs https://app.dimensions.ai/details/publication/pub.1022031124
124 rdf:type schema:CreativeWork
125 https://doi.org/10.1073/pnas.71.10.3874 schema:sameAs https://app.dimensions.ai/details/publication/pub.1013727357
126 rdf:type schema:CreativeWork
127 https://doi.org/10.1073/pnas.72.2.447 schema:sameAs https://app.dimensions.ai/details/publication/pub.1044786319
128 rdf:type schema:CreativeWork
129 https://doi.org/10.1101/sqb.1974.039.01.113 schema:sameAs https://app.dimensions.ai/details/publication/pub.1060404506
130 rdf:type schema:CreativeWork
 




Preview window. Press ESC to close (or click here)


...