Rapid quantitation of membrane antigens View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

1975-03

AUTHORS

K. I. WELSH, G. DORVAL, H. WIGZELL

ABSTRACT

STUDIES of cell-surface antigens are important in several fields of biological research, notably transplantation and tumour research. The most commonly used tests for such antigens involve assays requiring complements, and the subsequent measurement of cellular damage by the release of radioactive isotopes or the uptake of supravital dyes. Radioimmunoassays which measure directly the antibody–antigen reaction in such studies would have considerable advantages. Although it is possible to label antibody molecules directly, such attempts have so far been unsuccessful because of high background binding values. Furthermore, variations in the composition of the antisera would make each antiserum a unique target for radiolabelling with consequent variations in results. We have tried to circumvent this using a radiolabelled homogeneous marker for IgG molecules produced by certain bacteria. More... »

PAGES

67-69

Identifiers

URI

http://scigraph.springernature.com/pub.10.1038/254067a0

DOI

http://dx.doi.org/10.1038/254067a0

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1045259779

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/46592


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