A simple cytochemical technique for demonstration of DNA in cells infected with mycoplasmas and viruses View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

1975-02

AUTHORS

W. C. RUSSELL, CAROL NEWMAN, D. H. WILLIAMSON

ABSTRACT

4′-6-DIAMIDINO-2-PHENYLINDOLE (DAPI), which was first synthesised by Dann et al.1 as a trypanocide related to Berenil, has been shown to possess useful DNA binding properties2. Thus, DAPI will bind differentially to yeast mitochondrial and nuclear DNA forming highly fluorescent complexes and enhancing the separation of the two DNAs in caesium chloride gradients2. DAPI can also be used as a highly specific fluorescent stain for both nuclear and mitochondrial DNA in yeast. It seems to be highly sensitive and probably permits detection of a single yeast mitochondrial DNA molecule (D.H.W., and D. J. Fennell, unpublished). More... »

PAGES

461-462

Identifiers

URI

http://scigraph.springernature.com/pub.10.1038/253461a0

DOI

http://dx.doi.org/10.1038/253461a0

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1044188868

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/46112


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