Factors influencing microculture of leukaemia cells View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

1974-10

AUTHORS

F. BALKWILL, A. PINDAR, D. CROWTHER

ABSTRACT

THE culture of normal haemopoietic cells was accomplished1 by obtaining colonies of granulocytic and monocytic cells from mouse bone marrow grown in a semisolid agar system. The method was modified for human bone marrow and subsequent studies showed that leukaemia cells from the peripheral blood and marrow of patients with acute myelogenous leukaemia (AML) produced small colonies or clusters1,2. Using this technique, serial sampling and morphological or biochemical studies of the growing cells was difficult. A liquid culture technique was then developed to study the growth of granulocytic or monocytic cells from normal bone marrow3. This in vitro diffusion chamber technique had the advantage of easy sampling but involved the use of large culture vessels. The method has subsequently been used to cultivate AML cells4. More... »

PAGES

741-742

References to SciGraph publications

  • 1966-06. THE GROWTH OF MOUSE BONE MARROW CELLS IN VITRO in IMMUNOLOGY AND CELL BIOLOGY
  • Identifiers

    URI

    http://scigraph.springernature.com/pub.10.1038/251741a0

    DOI

    http://dx.doi.org/10.1038/251741a0

    DIMENSIONS

    https://app.dimensions.ai/details/publication/pub.1004374869

    PUBMED

    https://www.ncbi.nlm.nih.gov/pubmed/4530157


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