Intensity Fluctuation Spectroscopy of Motile Microorganisms View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

1974-03

AUTHORS

DALE W. SCHAEFER, GOON NKS, SEYMOUR S. ALPERT

ABSTRACT

LIGHT microscopy indicates that in Escherichia coli translational motility (‘running’) is accompanied by considerable side-to-side or wobble motion. Moreover, in the case of chemotactic strains Berg and Brown1 showed that a typical bacterium spends considerable time in a ‘twiddling’ state in which translation ceases in favour of jittering rotational motion. In both the running and twiddling state the amplitude of the non-translational component of motion is comparable with λ, the wavelength of light. It is reasonable to expect therefore that the dynamics of both wobble and twiddle motion can be studied by intensity fluctuation spectroscopy2 (IFS) of scattered laser light. We have examined the effects of wobble motion on the time dependence of the fluctuating light intensity scattered by a non-chemotactic mutant of E. coli whose motion is dominated by running with almost no twiddling. We conclude that IFS is sensitive primarily to the rotational component of the motion. More... »

PAGES

162-164

Identifiers

URI

http://scigraph.springernature.com/pub.10.1038/248162a0

DOI

http://dx.doi.org/10.1038/248162a0

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1051804292

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/4594530


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