A new logic for DNA engineering using recombination in Escherichia coli View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

1998-10

AUTHORS

Y Zhang, F Buchholz, J P Muyrers, A F Stewart

ABSTRACT

A straightforward way to engineer DNA in E. coli using homologous recombination is described. The homologous recombination reaction uses RecE and RecT and is transferable between E. coli strains. Several target molecules were manipulated, including high copy plasmids, a large episome and the E. coli chromosome. Sequential steps of homologous or site-specific recombination were used to demonstrate a new logic for engineering DNA, unlimited by the disposition of restriction endonuclease cleavage sites or the size of the target DNA. More... »

PAGES

123-128

Journal

TITLE

Nature Genetics

ISSUE

2

VOLUME

20

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  • Identifiers

    URI

    http://scigraph.springernature.com/pub.10.1038/2417

    DOI

    http://dx.doi.org/10.1038/2417

    DIMENSIONS

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    PUBMED

    https://www.ncbi.nlm.nih.gov/pubmed/9771703


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