Continued RAG expression in late stages of B cell development and no apparent re-induction after immunizion View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

1999-08

AUTHORS

Wong Yu, Hitoshi Nagaoka, Mila Jankovic, Ziva Misulovin, Heikyung Suh, Antonius Rolink, Fritz Melchers, Eric Meffre, Michel C. Nussenzweig

ABSTRACT

Models of B-cell development in the immune system suggest that only those immature B cells in the bone marrow that undergo receptor editing express V(D)J-recombination-activating genes (RAGs). Here we investigate the regulation of RAG expression in transgenic mice carrying a bacterial artificial chromosome that encodes a green fluorescent protein reporter instead of RAG2. We find that the reporter is expressed in all immature B cells in the bone marrow and spleen. Endogenous RAG messenger RNA is expressed in immature B cells in bone marrow and spleen and decreases by two orders of magnitude as they acquire higher levels of surface immunoglobulin M (IgM). Once RAG expression is stopped it is not re-induced during immune responses. Our findings may help to reconcile a series of apparently contradictory observations, and suggest a new model for the mechanisms that regulate allelic exclusion, receptor editing and tolerance. More... »

PAGES

682-687

Identifiers

URI

http://scigraph.springernature.com/pub.10.1038/23287

DOI

http://dx.doi.org/10.1038/23287

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1017730478

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/10458165


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