Linkage of Retinal to Opsin and Absence of Phospholipids in Purified Frog Visual Pigment500 View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

1970-02

AUTHORS

MICHAEL O. HALL, A. D. E. CHARACH

ABSTRACT

VISUAL pigments are components of the membranous disks of the rod and cone outer segments of vertebrates1. The traditional picture of visual pigment500 (rhodopsin) is a chromophore (retinal) bound to opsin through a Schiff base linkage2,3. A role has long been sought for the large quantities of phospholipids present in outer segment disks, however, and it has been proposed that, in native visual pigment, retinal is bound by a Schiff base linkage to phosphatidyl ethanolamine (PE) rather than to opsin4–6. When exposed to light, retinal is considered to migrate from PE to the ε-amino group of a lysine residue in opsin, but Heller7 has found that purified visual pigment contains little or no phospholipid (<2 per cent). The following experiments are intended to resolve this contradiction; we have used the recent observation that visual pigment in the outer segment disks is continually being renewed8,9, to show that purified visual pigment contains no phospholipid. More... »

PAGES

637

Identifiers

URI

http://scigraph.springernature.com/pub.10.1038/225637a0

DOI

http://dx.doi.org/10.1038/225637a0

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1050265299

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/5413371


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