Ontology type: schema:ScholarlyArticle
1969-08
AUTHORS ABSTRACTIT was shown1 that strips of immature calf uterus incubated in vitro in the presence of 10−9 M 3H-oestradiol-17β concentrate the hormone 400 per cent or more; about 80 per cent of the radioactivity in the tissue is found associated strongly but non-covalently with the nuclear fraction and consists of intact 17β-oestradiol; treatment of the nuclear fraction with 0.4 M KCl at pH 8.5, as already applied by us to rat2, solubilizes an oestradiol-macromolecule complex which sediments at about 5S; the macromolecule is protein, or at least the moiety binding the hormone is protein. A similar 5S oestrophilic component from calf uterus was obtained by Jungblut et al.3. Recent studies4–6 suggest that the 5S macromolecule originates from the 8–10S macromolecular component which binds 17β-oestradiol and is found in the high speed supernatant of uterus. More... »
PAGES745-747
http://scigraph.springernature.com/pub.10.1038/223745a0
DOIhttp://dx.doi.org/10.1038/223745a0
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PUBMEDhttps://www.ncbi.nlm.nih.gov/pubmed/5802686
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