Isolation and Characterization of a Streptolydigin Resistant RNA Polymerase View Full Text


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Article Info

DATE

1969-09

AUTHORS

ROBERT SCHLEIF

ABSTRACT

THE RNA polymerase of Escherichia coli consists of two small subunits α, two larger subunits β and β′, and one subunit σ which aids initiation1. A question relevant to control of polymerase synthesis is whether genes coding for the various subunits are contiguous. Both rifampicin resistant RNA polymerase and streptovaricin resistant RNA polymerase map near the argH locus2,3. Here I report that (a) streptolydigin inhibits chain elongation by E. coli RNA polymerase as was found by Siddhikol et al.4 for the polymerase of B. megaterium, (b) mutants altering the polymerase to streptolydigin resistance also map near argH, and (c) that, as is the case of rifampicin resistance1,2, the “core” enzyme (the α2ββ′ structure which itself possesses enzymatic activity) is modified in the resistant mutant. More... »

PAGES

1068-1069

Identifiers

URI

http://scigraph.springernature.com/pub.10.1038/2231068a0

DOI

http://dx.doi.org/10.1038/2231068a0

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1010883774

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/4897821


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