Isolation of Chromatin-free RNA Polymerase from Mammalian Cell Nuclei View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

1968-12

AUTHORS

SHUTSUNG LIAO, DAPHNA SAGHER, SEN-MAW FANG

ABSTRACT

MAMMALIAN RNA polymerase1 has been obtained in a soluble form from tumours2,3, chick embryo4, rat testis5 and rat liver nuclei6,7. The extraction procedures used involved the disruption of cell nuclei by sonication or exposure either to a hypotonic solution or alkaline pH. As a consequence, extracts were grossly contaminated with nuclear DNA and other cellular components and the yields of the enzyme were usually very low. We describe here a procedure for the rapid extraction from liver nuclei of fairly large yields of soluble RNA polymerase with fairly high specific activity. More... »

PAGES

1336-1337

Identifiers

URI

http://scigraph.springernature.com/pub.10.1038/2201336a0

DOI

http://dx.doi.org/10.1038/2201336a0

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1010913800

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/5701354


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