Synthesis of Cellulose by Enzyme Preparations from the Developing Cotton Boll View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

1965-07

AUTHORS

G. A. BARBER, W. Z. HASSID

ABSTRACT

RECENTLY an enzyme system from mung bean seedlings was described which catalyses the incorporation of radio-activity into cellulose from guanosine diphosphate D-glucose labelled with 14C in the D-glucosyl moiety1. The extent of this incorporation was considerably stimu lated by the addition of GDP-D-mannose to the reaction mixtuies. This effect was later shown to result from the formation of some as yet uncharacterized polysaccharide, probably a glucomannan2. The synthesis of this compound also appeared to proceed at a rate many times that at which cellulose was formed by the same preparations. Sugars other than D-glucose, chiefly D-xylose or D-mannose, have been found associated with the cellulosic fractions of most plant species, but cotton fibres consist almost entirely of β-1,4 linked D-glucosyl residues3. It was therefore of interest to learn whether 14C-labelled D-glucose from GDP-D-glucose 14C could be incorporated into the cellulose of cotton fibres in vitro and whether other polysaccharides were formed by the same cell-free enzyme system. The experiments reported here demonstrate that both these processes are brought about by enzymes isolated from the developing cotton boll. More... »

PAGES

295-296

References to SciGraph publications

Identifiers

URI

http://scigraph.springernature.com/pub.10.1038/207295b0

DOI

http://dx.doi.org/10.1038/207295b0

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1008891414

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/5899273


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