Biosynthesis of Ethylene View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

1964-08

AUTHORS

STANLEY P. BURG, ELLEN A. BURG

ABSTRACT

IT is the purpose of the present communication to describe some further results with carbon-14 precursors of ethylene, and to reconcile the pattern which emerges in these investigations using McIntosh apple tissue sections with that of the fungal system, Penicillium digitatum. The procedures have been described previously1–3, and their reliability for measuring 14C-ethylene and carbon dioxide commented on3; they were altered only in the use of a Cary–Tolbert ionization chamber in place of a Bernstein–Ballentine tube for measuring radiation. In the course of these experiments it was noted that alcohol vapour interferes with the assay for ethylene by dissolving in mercuric perchlorate absorbent where a slow acid-catalysed dehydration occurs. For example, when 10 µl. liquid ethanol was added to 20 ml. of mercuric perchlorate reagent, ethylene appeared at a linear rate for at least 6 h, by which time 0.02 per cent of the alcohol had been converted to olefine. Previously, Young et al.9 reported that ethanol was not measured by the mercuric perchlorate manometric procedure, but they apparently took no account of the duration of contact between alcohol and perchlorate, and it should be noted that gas chromatography detects much smaller quantities of vapour than does manometry. In the work recorded here it was determined that a discernible error could occur only if at the end of a collection period in excess of 1 µc. of 14C-ethanol had been formed in the tissue. In experiments with fumarate and acetate this would require that labelled ethanol form at approximately the same rate as 14CO2, and with other compounds ethanol production would greatly have to exceed 14CO2 evolution. Clearly, under the conditions of the assay, the results are not influenced by this factor except when 14C-ethanol itself was administered to the tissue (Table 1), in which case the apparent transfer of label to ethylene must be dismissed as an artefact. More... »

PAGES

869-870

References to SciGraph publications

Identifiers

URI

http://scigraph.springernature.com/pub.10.1038/203869a0

DOI

http://dx.doi.org/10.1038/203869a0

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1021633676

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/14204075


Indexing Status Check whether this publication has been indexed by Scopus and Web Of Science using the SN Indexing Status Tool
Incoming Citations Browse incoming citations for this publication using opencitations.net

JSON-LD is the canonical representation for SciGraph data.

TIP: You can open this SciGraph record using an external JSON-LD service: JSON-LD Playground Google SDTT

[
  {
    "@context": "https://springernature.github.io/scigraph/jsonld/sgcontext.json", 
    "about": [
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/06", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Biological Sciences", 
        "type": "DefinedTerm"
      }, 
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/0601", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Biochemistry and Cell Biology", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Carbohydrate Metabolism", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Carbon Dioxide", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Carbon Isotopes", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Chromatography", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Citric Acid Cycle", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Ethylenes", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Fruit", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Glucose", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Manometry", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Metabolism", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Penicillium", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Research", 
        "type": "DefinedTerm"
      }
    ], 
    "author": [
      {
        "affiliation": {
          "alternateName": "Department of Physiology, University of Miami School of Medicine, and Howard Hughes Medical Institute, Miami, Florida", 
          "id": "http://www.grid.ac/institutes/grid.26790.3a", 
          "name": [
            "Department of Physiology, University of Miami School of Medicine, and Howard Hughes Medical Institute, Miami, Florida"
          ], 
          "type": "Organization"
        }, 
        "familyName": "BURG", 
        "givenName": "STANLEY P.", 
        "id": "sg:person.0103205143.83", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0103205143.83"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Department of Physiology, University of Miami School of Medicine, and Howard Hughes Medical Institute, Miami, Florida", 
          "id": "http://www.grid.ac/institutes/grid.26790.3a", 
          "name": [
            "Department of Physiology, University of Miami School of Medicine, and Howard Hughes Medical Institute, Miami, Florida"
          ], 
          "type": "Organization"
        }, 
        "familyName": "BURG", 
        "givenName": "ELLEN A.", 
        "id": "sg:person.055552036.18", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.055552036.18"
        ], 
        "type": "Person"
      }
    ], 
    "citation": [
      {
        "id": "sg:pub.10.1038/1951306a0", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1014976012", 
          "https://doi.org/10.1038/1951306a0"
        ], 
        "type": "CreativeWork"
      }
    ], 
    "datePublished": "1964-08", 
    "datePublishedReg": "1964-08-01", 
    "description": "IT is the purpose of the present communication to describe some further results with carbon-14 precursors of ethylene, and to reconcile the pattern which emerges in these investigations using McIntosh apple tissue sections with that of the fungal system, Penicillium digitatum. The procedures have been described previously1\u20133, and their reliability for measuring 14C-ethylene and carbon dioxide commented on3; they were altered only in the use of a Cary\u2013Tolbert ionization chamber in place of a Bernstein\u2013Ballentine tube for measuring radiation. In the course of these experiments it was noted that alcohol vapour interferes with the assay for ethylene by dissolving in mercuric perchlorate absorbent where a slow acid-catalysed dehydration occurs. For example, when 10 \u00b5l. liquid ethanol was added to 20 ml. of mercuric perchlorate reagent, ethylene appeared at a linear rate for at least 6 h, by which time 0.02 per cent of the alcohol had been converted to olefine. Previously, Young et al.9 reported that ethanol was not measured by the mercuric perchlorate manometric procedure, but they apparently took no account of the duration of contact between alcohol and perchlorate, and it should be noted that gas chromatography detects much smaller quantities of vapour than does manometry. In the work recorded here it was determined that a discernible error could occur only if at the end of a collection period in excess of 1 \u00b5c. of 14C-ethanol had been formed in the tissue. In experiments with fumarate and acetate this would require that labelled ethanol form at approximately the same rate as 14CO2, and with other compounds ethanol production would greatly have to exceed 14CO2 evolution. Clearly, under the conditions of the assay, the results are not influenced by this factor except when 14C-ethanol itself was administered to the tissue (Table 1), in which case the apparent transfer of label to ethylene must be dismissed as an artefact.", 
    "genre": "article", 
    "id": "sg:pub.10.1038/203869a0", 
    "isAccessibleForFree": false, 
    "isPartOf": [
      {
        "id": "sg:journal.1018957", 
        "issn": [
          "0028-0836", 
          "1476-4687"
        ], 
        "name": "Nature", 
        "publisher": "Springer Nature", 
        "type": "Periodical"
      }, 
      {
        "issueNumber": "4947", 
        "type": "PublicationIssue"
      }, 
      {
        "type": "PublicationVolume", 
        "volumeNumber": "203"
      }
    ], 
    "keywords": [
      "communication", 
      "experiments", 
      "alcohol", 
      "Young et al", 
      "labels", 
      "IT", 
      "purpose", 
      "present communication", 
      "Further results", 
      "results", 
      "precursors", 
      "ethylene", 
      "patterns", 
      "investigation", 
      "tissue sections", 
      "sections", 
      "system", 
      "Penicillium digitatum", 
      "digitatum", 
      "procedure", 
      "previously1\u20133", 
      "reliability", 
      "carbon dioxide", 
      "dioxide", 
      "ON3", 
      "use", 
      "ionization chamber", 
      "chamber", 
      "place", 
      "tube", 
      "radiation", 
      "course", 
      "alcohol vapor", 
      "vapor", 
      "assays", 
      "absorbent", 
      "acid-catalyzed dehydration", 
      "dehydration", 
      "example", 
      "liquid ethanol", 
      "ethanol", 
      "reagents", 
      "linear rate", 
      "rate", 
      "cent", 
      "et al", 
      "al", 
      "manometric procedure", 
      "account", 
      "duration of contact", 
      "duration", 
      "contact", 
      "perchlorate", 
      "gas chromatography", 
      "chromatography", 
      "small quantities", 
      "quantity", 
      "manometry", 
      "work", 
      "error", 
      "end", 
      "collection period", 
      "period", 
      "excess", 
      "tissue", 
      "fumarate", 
      "ethanol forms", 
      "form", 
      "same rate", 
      "ethanol production", 
      "production", 
      "evolution", 
      "conditions", 
      "factors", 
      "cases", 
      "apparent transfer", 
      "transfer", 
      "artifacts", 
      "biosynthesis", 
      "carbon-14 precursors", 
      "McIntosh apple tissue sections", 
      "apple tissue sections", 
      "fungal systems", 
      "Cary\u2013Tolbert ionization chamber", 
      "Bernstein\u2013Ballentine tube", 
      "mercuric perchlorate absorbent", 
      "perchlorate absorbent", 
      "slow acid-catalysed dehydration", 
      "mercuric perchlorate reagent", 
      "perchlorate reagent", 
      "mercuric perchlorate manometric procedure", 
      "perchlorate manometric procedure", 
      "discernible error", 
      "compounds ethanol production", 
      "biosynthesis of ethylene"
    ], 
    "name": "Biosynthesis of Ethylene", 
    "pagination": "869-870", 
    "productId": [
      {
        "name": "dimensions_id", 
        "type": "PropertyValue", 
        "value": [
          "pub.1021633676"
        ]
      }, 
      {
        "name": "doi", 
        "type": "PropertyValue", 
        "value": [
          "10.1038/203869a0"
        ]
      }, 
      {
        "name": "pubmed_id", 
        "type": "PropertyValue", 
        "value": [
          "14204075"
        ]
      }
    ], 
    "sameAs": [
      "https://doi.org/10.1038/203869a0", 
      "https://app.dimensions.ai/details/publication/pub.1021633676"
    ], 
    "sdDataset": "articles", 
    "sdDatePublished": "2022-01-01T19:04", 
    "sdLicense": "https://scigraph.springernature.com/explorer/license/", 
    "sdPublisher": {
      "name": "Springer Nature - SN SciGraph project", 
      "type": "Organization"
    }, 
    "sdSource": "s3://com-springernature-scigraph/baseset/20220101/entities/gbq_results/article/article_97.jsonl", 
    "type": "ScholarlyArticle", 
    "url": "https://doi.org/10.1038/203869a0"
  }
]
 

Download the RDF metadata as:  json-ld nt turtle xml License info

HOW TO GET THIS DATA PROGRAMMATICALLY:

JSON-LD is a popular format for linked data which is fully compatible with JSON.

curl -H 'Accept: application/ld+json' 'https://scigraph.springernature.com/pub.10.1038/203869a0'

N-Triples is a line-based linked data format ideal for batch operations.

curl -H 'Accept: application/n-triples' 'https://scigraph.springernature.com/pub.10.1038/203869a0'

Turtle is a human-readable linked data format.

curl -H 'Accept: text/turtle' 'https://scigraph.springernature.com/pub.10.1038/203869a0'

RDF/XML is a standard XML format for linked data.

curl -H 'Accept: application/rdf+xml' 'https://scigraph.springernature.com/pub.10.1038/203869a0'


 

This table displays all metadata directly associated to this object as RDF triples.

215 TRIPLES      21 PREDICATES      134 URIs      125 LITERALS      19 BLANK NODES

Subject Predicate Object
1 sg:pub.10.1038/203869a0 schema:about N06817f2165c242a19d6eaeaaa450a252
2 N2650fa3642a34fc09f113aae2e22f005
3 N2c7621d8a34d4d9d839b7e4e0fba86f6
4 N4fe01334a664497baafb3cd5d558d819
5 N525b2f8caed3430bbf2fe5c13db53dc8
6 N983d9d4f66c04920a45c8ca1b8fce5d0
7 Nc8479d80ae7345b7b61815b0a326e187
8 Nd27a0dd6e8b1418c9c8b6284ab7d2c17
9 Nd667e80193d747a184e6aac14151e29f
10 Ne0c74dfe60814b619271c5d364bb1ed8
11 Ne7b73e5d5c3146bb9d827d907d23b80e
12 Nff9044d9e0624a4594b264983297db98
13 anzsrc-for:06
14 anzsrc-for:0601
15 schema:author N6138d9593a27452d8bd9c3e5d9cc5199
16 schema:citation sg:pub.10.1038/1951306a0
17 schema:datePublished 1964-08
18 schema:datePublishedReg 1964-08-01
19 schema:description IT is the purpose of the present communication to describe some further results with carbon-14 precursors of ethylene, and to reconcile the pattern which emerges in these investigations using McIntosh apple tissue sections with that of the fungal system, Penicillium digitatum. The procedures have been described previously1–3, and their reliability for measuring 14C-ethylene and carbon dioxide commented on3; they were altered only in the use of a Cary–Tolbert ionization chamber in place of a Bernstein–Ballentine tube for measuring radiation. In the course of these experiments it was noted that alcohol vapour interferes with the assay for ethylene by dissolving in mercuric perchlorate absorbent where a slow acid-catalysed dehydration occurs. For example, when 10 µl. liquid ethanol was added to 20 ml. of mercuric perchlorate reagent, ethylene appeared at a linear rate for at least 6 h, by which time 0.02 per cent of the alcohol had been converted to olefine. Previously, Young et al.9 reported that ethanol was not measured by the mercuric perchlorate manometric procedure, but they apparently took no account of the duration of contact between alcohol and perchlorate, and it should be noted that gas chromatography detects much smaller quantities of vapour than does manometry. In the work recorded here it was determined that a discernible error could occur only if at the end of a collection period in excess of 1 µc. of 14C-ethanol had been formed in the tissue. In experiments with fumarate and acetate this would require that labelled ethanol form at approximately the same rate as 14CO2, and with other compounds ethanol production would greatly have to exceed 14CO2 evolution. Clearly, under the conditions of the assay, the results are not influenced by this factor except when 14C-ethanol itself was administered to the tissue (Table 1), in which case the apparent transfer of label to ethylene must be dismissed as an artefact.
20 schema:genre article
21 schema:isAccessibleForFree false
22 schema:isPartOf Nb0c95d83193e4e1fb113461bf678298d
23 Nc6fa0135b16b45f8aa3d60222bff408b
24 sg:journal.1018957
25 schema:keywords Bernstein–Ballentine tube
26 Cary–Tolbert ionization chamber
27 Further results
28 IT
29 McIntosh apple tissue sections
30 ON3
31 Penicillium digitatum
32 Young et al
33 absorbent
34 account
35 acid-catalyzed dehydration
36 al
37 alcohol
38 alcohol vapor
39 apparent transfer
40 apple tissue sections
41 artifacts
42 assays
43 biosynthesis
44 biosynthesis of ethylene
45 carbon dioxide
46 carbon-14 precursors
47 cases
48 cent
49 chamber
50 chromatography
51 collection period
52 communication
53 compounds ethanol production
54 conditions
55 contact
56 course
57 dehydration
58 digitatum
59 dioxide
60 discernible error
61 duration
62 duration of contact
63 end
64 error
65 et al
66 ethanol
67 ethanol forms
68 ethanol production
69 ethylene
70 evolution
71 example
72 excess
73 experiments
74 factors
75 form
76 fumarate
77 fungal systems
78 gas chromatography
79 investigation
80 ionization chamber
81 labels
82 linear rate
83 liquid ethanol
84 manometric procedure
85 manometry
86 mercuric perchlorate absorbent
87 mercuric perchlorate manometric procedure
88 mercuric perchlorate reagent
89 patterns
90 perchlorate
91 perchlorate absorbent
92 perchlorate manometric procedure
93 perchlorate reagent
94 period
95 place
96 precursors
97 present communication
98 previously1–3
99 procedure
100 production
101 purpose
102 quantity
103 radiation
104 rate
105 reagents
106 reliability
107 results
108 same rate
109 sections
110 slow acid-catalysed dehydration
111 small quantities
112 system
113 tissue
114 tissue sections
115 transfer
116 tube
117 use
118 vapor
119 work
120 schema:name Biosynthesis of Ethylene
121 schema:pagination 869-870
122 schema:productId N2e09b20d281c4d15a1ba92ecd111c0c6
123 Nd2dc9886cc944d40927feab79c717a4c
124 Ned13b12a569d4db0bfc45deb1d2e1b3d
125 schema:sameAs https://app.dimensions.ai/details/publication/pub.1021633676
126 https://doi.org/10.1038/203869a0
127 schema:sdDatePublished 2022-01-01T19:04
128 schema:sdLicense https://scigraph.springernature.com/explorer/license/
129 schema:sdPublisher Ncb14033d0e8a46be9f2ee6e1a55fffbf
130 schema:url https://doi.org/10.1038/203869a0
131 sgo:license sg:explorer/license/
132 sgo:sdDataset articles
133 rdf:type schema:ScholarlyArticle
134 N06817f2165c242a19d6eaeaaa450a252 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
135 schema:name Fruit
136 rdf:type schema:DefinedTerm
137 N2650fa3642a34fc09f113aae2e22f005 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
138 schema:name Penicillium
139 rdf:type schema:DefinedTerm
140 N2c7621d8a34d4d9d839b7e4e0fba86f6 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
141 schema:name Ethylenes
142 rdf:type schema:DefinedTerm
143 N2e09b20d281c4d15a1ba92ecd111c0c6 schema:name doi
144 schema:value 10.1038/203869a0
145 rdf:type schema:PropertyValue
146 N4fe01334a664497baafb3cd5d558d819 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
147 schema:name Research
148 rdf:type schema:DefinedTerm
149 N525b2f8caed3430bbf2fe5c13db53dc8 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
150 schema:name Carbohydrate Metabolism
151 rdf:type schema:DefinedTerm
152 N6138d9593a27452d8bd9c3e5d9cc5199 rdf:first sg:person.0103205143.83
153 rdf:rest Ne2eaf3304fd1440b83c1110df4522d62
154 N983d9d4f66c04920a45c8ca1b8fce5d0 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
155 schema:name Chromatography
156 rdf:type schema:DefinedTerm
157 Nb0c95d83193e4e1fb113461bf678298d schema:volumeNumber 203
158 rdf:type schema:PublicationVolume
159 Nc6fa0135b16b45f8aa3d60222bff408b schema:issueNumber 4947
160 rdf:type schema:PublicationIssue
161 Nc8479d80ae7345b7b61815b0a326e187 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
162 schema:name Glucose
163 rdf:type schema:DefinedTerm
164 Ncb14033d0e8a46be9f2ee6e1a55fffbf schema:name Springer Nature - SN SciGraph project
165 rdf:type schema:Organization
166 Nd27a0dd6e8b1418c9c8b6284ab7d2c17 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
167 schema:name Manometry
168 rdf:type schema:DefinedTerm
169 Nd2dc9886cc944d40927feab79c717a4c schema:name pubmed_id
170 schema:value 14204075
171 rdf:type schema:PropertyValue
172 Nd667e80193d747a184e6aac14151e29f schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
173 schema:name Metabolism
174 rdf:type schema:DefinedTerm
175 Ne0c74dfe60814b619271c5d364bb1ed8 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
176 schema:name Carbon Isotopes
177 rdf:type schema:DefinedTerm
178 Ne2eaf3304fd1440b83c1110df4522d62 rdf:first sg:person.055552036.18
179 rdf:rest rdf:nil
180 Ne7b73e5d5c3146bb9d827d907d23b80e schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
181 schema:name Citric Acid Cycle
182 rdf:type schema:DefinedTerm
183 Ned13b12a569d4db0bfc45deb1d2e1b3d schema:name dimensions_id
184 schema:value pub.1021633676
185 rdf:type schema:PropertyValue
186 Nff9044d9e0624a4594b264983297db98 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
187 schema:name Carbon Dioxide
188 rdf:type schema:DefinedTerm
189 anzsrc-for:06 schema:inDefinedTermSet anzsrc-for:
190 schema:name Biological Sciences
191 rdf:type schema:DefinedTerm
192 anzsrc-for:0601 schema:inDefinedTermSet anzsrc-for:
193 schema:name Biochemistry and Cell Biology
194 rdf:type schema:DefinedTerm
195 sg:journal.1018957 schema:issn 0028-0836
196 1476-4687
197 schema:name Nature
198 schema:publisher Springer Nature
199 rdf:type schema:Periodical
200 sg:person.0103205143.83 schema:affiliation grid-institutes:grid.26790.3a
201 schema:familyName BURG
202 schema:givenName STANLEY P.
203 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0103205143.83
204 rdf:type schema:Person
205 sg:person.055552036.18 schema:affiliation grid-institutes:grid.26790.3a
206 schema:familyName BURG
207 schema:givenName ELLEN A.
208 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.055552036.18
209 rdf:type schema:Person
210 sg:pub.10.1038/1951306a0 schema:sameAs https://app.dimensions.ai/details/publication/pub.1014976012
211 https://doi.org/10.1038/1951306a0
212 rdf:type schema:CreativeWork
213 grid-institutes:grid.26790.3a schema:alternateName Department of Physiology, University of Miami School of Medicine, and Howard Hughes Medical Institute, Miami, Florida
214 schema:name Department of Physiology, University of Miami School of Medicine, and Howard Hughes Medical Institute, Miami, Florida
215 rdf:type schema:Organization
 




Preview window. Press ESC to close (or click here)


...