Increased Rate of Plaque-type and Host-range Mutation following Treatment of Bacteriophage in vitro with Ethyl Methane Sulphonate View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

1958-04

AUTHORS

ANTHONY LOVELESS

ABSTRACT

ALTHOUGH bacteriophage undergoes ‘spontaneous mutation during the course of intracellular multiplication, it has proved difficult to increase the mutation-rate (that is, to ‘induce’ mutation) by artificial means. Latarjet1 has presented evidence for an increased proportion of host-range mutants in the progeny of bacteria infected with phage T2 as a result of ultraviolet irradiation of the complex. The proportion of host-range mutants of T1 was found by Tessman2 to be increased when both host cells and virus were irradiated with ultra-violet prior to infection, but not when either component alone was irradiated. De Mars3 found an increase in the yield of plaque-type mutants when euflavine was administered during intracellular growth, and Litman and Pardee4 achieved a similar result when phage was grown through a number of cycles in a medium containing bromouracil. No increase in the mutation-rate of phage has hitherto been reported which was the consequence of treatment of the phage alone prior to infection of the host cells. I have now been able to show that treatment of phage T2 in vitro with the ethylating agent ethyl methane sulphonate results in an increase in the number of mutations to the r and h genotype in the first cycle of infection of the (untreated) host organism Escherichia coli B. More... »

PAGES

1212-1213

Identifiers

URI

http://scigraph.springernature.com/pub.10.1038/1811212a0

DOI

http://dx.doi.org/10.1038/1811212a0

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1050023144

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/13541432


Indexing Status Check whether this publication has been indexed by Scopus and Web Of Science using the SN Indexing Status Tool
Incoming Citations Browse incoming citations for this publication using opencitations.net

JSON-LD is the canonical representation for SciGraph data.

TIP: You can open this SciGraph record using an external JSON-LD service: JSON-LD Playground Google SDTT

[
  {
    "@context": "https://springernature.github.io/scigraph/jsonld/sgcontext.json", 
    "about": [
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/11", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Medical and Health Sciences", 
        "type": "DefinedTerm"
      }, 
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/1108", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Medical Microbiology", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Bacteriophages", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Ethyl Methanesulfonate", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Host Specificity", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "In Vitro Techniques", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Methane", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Mutation", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Plaque, Amyloid", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Sulfonic Acids", 
        "type": "DefinedTerm"
      }
    ], 
    "author": [
      {
        "affiliation": {
          "alternateName": "Chester Beatty Research Institute, Institute of Cancer Research, Royal Cancer Hospital, Fulham Road, S.W.3, London", 
          "id": "http://www.grid.ac/institutes/grid.424926.f", 
          "name": [
            "Chester Beatty Research Institute, Institute of Cancer Research, Royal Cancer Hospital, Fulham Road, S.W.3, London"
          ], 
          "type": "Organization"
        }, 
        "familyName": "LOVELESS", 
        "givenName": "ANTHONY", 
        "type": "Person"
      }
    ], 
    "citation": [
      {
        "id": "sg:pub.10.1038/172964a0", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1046643116", 
          "https://doi.org/10.1038/172964a0"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/178529b0", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1030249739", 
          "https://doi.org/10.1038/178529b0"
        ], 
        "type": "CreativeWork"
      }
    ], 
    "datePublished": "1958-04", 
    "datePublishedReg": "1958-04-01", 
    "description": "ALTHOUGH bacteriophage undergoes \u2018spontaneous mutation during the course of intracellular multiplication, it has proved difficult to increase the mutation-rate (that is, to \u2018induce\u2019 mutation) by artificial means. Latarjet1 has presented evidence for an increased proportion of host-range mutants in the progeny of bacteria infected with phage T2 as a result of ultraviolet irradiation of the complex. The proportion of host-range mutants of T1 was found by Tessman2 to be increased when both host cells and virus were irradiated with ultra-violet prior to infection, but not when either component alone was irradiated. De Mars3 found an increase in the yield of plaque-type mutants when euflavine was administered during intracellular growth, and Litman and Pardee4 achieved a similar result when phage was grown through a number of cycles in a medium containing bromouracil. No increase in the mutation-rate of phage has hitherto been reported which was the consequence of treatment of the phage alone prior to infection of the host cells. I have now been able to show that treatment of phage T2 in vitro with the ethylating agent ethyl methane sulphonate results in an increase in the number of mutations to the r and h genotype in the first cycle of infection of the (untreated) host organism Escherichia coli B.", 
    "genre": "article", 
    "id": "sg:pub.10.1038/1811212a0", 
    "isAccessibleForFree": false, 
    "isPartOf": [
      {
        "id": "sg:journal.1018957", 
        "issn": [
          "0028-0836", 
          "1476-4687"
        ], 
        "name": "Nature", 
        "publisher": "Springer Nature", 
        "type": "Periodical"
      }, 
      {
        "issueNumber": "4617", 
        "type": "PublicationIssue"
      }, 
      {
        "type": "PublicationVolume", 
        "volumeNumber": "181"
      }
    ], 
    "keywords": [
      "consequence of treatment", 
      "host cells", 
      "plaque-type mutants", 
      "host range mutants", 
      "infection", 
      "increased proportion", 
      "intracellular multiplication", 
      "intracellular growth", 
      "increased rates", 
      "treatment", 
      "host range mutations", 
      "number of mutations", 
      "mutations", 
      "similar results", 
      "cells", 
      "T2", 
      "proportion", 
      "first cycle", 
      "increase", 
      "virus", 
      "spontaneous mutations", 
      "phage T2", 
      "ultraviolet irradiation", 
      "T1", 
      "course", 
      "phages", 
      "genotypes", 
      "evidence", 
      "artificial means", 
      "number", 
      "results", 
      "rate", 
      "bacteria", 
      "mutants", 
      "methane sulphonate", 
      "cycle", 
      "euflavine", 
      "ethyl methane sulphonate", 
      "consequences", 
      "bacteriophages", 
      "ethyl methane", 
      "irradiation", 
      "progeny", 
      "growth", 
      "means", 
      "components", 
      "number of cycles", 
      "bromouracil", 
      "sulphonate", 
      "complexes", 
      "hitherto", 
      "undergoes", 
      "multiplication", 
      "Litman", 
      "yield", 
      "methane"
    ], 
    "name": "Increased Rate of Plaque-type and Host-range Mutation following Treatment of Bacteriophage in vitro with Ethyl Methane Sulphonate", 
    "pagination": "1212-1213", 
    "productId": [
      {
        "name": "dimensions_id", 
        "type": "PropertyValue", 
        "value": [
          "pub.1050023144"
        ]
      }, 
      {
        "name": "doi", 
        "type": "PropertyValue", 
        "value": [
          "10.1038/1811212a0"
        ]
      }, 
      {
        "name": "pubmed_id", 
        "type": "PropertyValue", 
        "value": [
          "13541432"
        ]
      }
    ], 
    "sameAs": [
      "https://doi.org/10.1038/1811212a0", 
      "https://app.dimensions.ai/details/publication/pub.1050023144"
    ], 
    "sdDataset": "articles", 
    "sdDatePublished": "2022-06-01T22:13", 
    "sdLicense": "https://scigraph.springernature.com/explorer/license/", 
    "sdPublisher": {
      "name": "Springer Nature - SN SciGraph project", 
      "type": "Organization"
    }, 
    "sdSource": "s3://com-springernature-scigraph/baseset/20220601/entities/gbq_results/article/article_67.jsonl", 
    "type": "ScholarlyArticle", 
    "url": "https://doi.org/10.1038/1811212a0"
  }
]
 

Download the RDF metadata as:  json-ld nt turtle xml License info

HOW TO GET THIS DATA PROGRAMMATICALLY:

JSON-LD is a popular format for linked data which is fully compatible with JSON.

curl -H 'Accept: application/ld+json' 'https://scigraph.springernature.com/pub.10.1038/1811212a0'

N-Triples is a line-based linked data format ideal for batch operations.

curl -H 'Accept: application/n-triples' 'https://scigraph.springernature.com/pub.10.1038/1811212a0'

Turtle is a human-readable linked data format.

curl -H 'Accept: text/turtle' 'https://scigraph.springernature.com/pub.10.1038/1811212a0'

RDF/XML is a standard XML format for linked data.

curl -H 'Accept: application/rdf+xml' 'https://scigraph.springernature.com/pub.10.1038/1811212a0'


 

This table displays all metadata directly associated to this object as RDF triples.

156 TRIPLES      21 PREDICATES      92 URIs      82 LITERALS      15 BLANK NODES

Subject Predicate Object
1 sg:pub.10.1038/1811212a0 schema:about N5b0a4aab53744621ad93501f9763d071
2 N5f52a1cb01ce44cfbe18b79be952ac35
3 N86fd9bbe2ce4418894c575683c3f557f
4 Na1cf3ede00ff44d7a67dab14aa388944
5 Nb5949cc48de044a2ac216822f42cd3ce
6 Nec22d6f3c02449d3ace1eba03ed01de4
7 Nf62ebf8b6bd741a8a59c6b535b871994
8 Nff3ee506dec24bdd8e52f82bb583d7b2
9 anzsrc-for:11
10 anzsrc-for:1108
11 schema:author Nfe12ba0f23964012ab50552a56f5c86c
12 schema:citation sg:pub.10.1038/172964a0
13 sg:pub.10.1038/178529b0
14 schema:datePublished 1958-04
15 schema:datePublishedReg 1958-04-01
16 schema:description ALTHOUGH bacteriophage undergoes ‘spontaneous mutation during the course of intracellular multiplication, it has proved difficult to increase the mutation-rate (that is, to ‘induce’ mutation) by artificial means. Latarjet1 has presented evidence for an increased proportion of host-range mutants in the progeny of bacteria infected with phage T2 as a result of ultraviolet irradiation of the complex. The proportion of host-range mutants of T1 was found by Tessman2 to be increased when both host cells and virus were irradiated with ultra-violet prior to infection, but not when either component alone was irradiated. De Mars3 found an increase in the yield of plaque-type mutants when euflavine was administered during intracellular growth, and Litman and Pardee4 achieved a similar result when phage was grown through a number of cycles in a medium containing bromouracil. No increase in the mutation-rate of phage has hitherto been reported which was the consequence of treatment of the phage alone prior to infection of the host cells. I have now been able to show that treatment of phage T2 in vitro with the ethylating agent ethyl methane sulphonate results in an increase in the number of mutations to the r and h genotype in the first cycle of infection of the (untreated) host organism Escherichia coli B.
17 schema:genre article
18 schema:isAccessibleForFree false
19 schema:isPartOf N4489039e4d814a7abc8ab0a563bbdcc4
20 N94d18b861ad64658ad9ff5a6da3090ef
21 sg:journal.1018957
22 schema:keywords Litman
23 T1
24 T2
25 artificial means
26 bacteria
27 bacteriophages
28 bromouracil
29 cells
30 complexes
31 components
32 consequence of treatment
33 consequences
34 course
35 cycle
36 ethyl methane
37 ethyl methane sulphonate
38 euflavine
39 evidence
40 first cycle
41 genotypes
42 growth
43 hitherto
44 host cells
45 host range mutants
46 host range mutations
47 increase
48 increased proportion
49 increased rates
50 infection
51 intracellular growth
52 intracellular multiplication
53 irradiation
54 means
55 methane
56 methane sulphonate
57 multiplication
58 mutants
59 mutations
60 number
61 number of cycles
62 number of mutations
63 phage T2
64 phages
65 plaque-type mutants
66 progeny
67 proportion
68 rate
69 results
70 similar results
71 spontaneous mutations
72 sulphonate
73 treatment
74 ultraviolet irradiation
75 undergoes
76 virus
77 yield
78 schema:name Increased Rate of Plaque-type and Host-range Mutation following Treatment of Bacteriophage in vitro with Ethyl Methane Sulphonate
79 schema:pagination 1212-1213
80 schema:productId N091cf1ebee364d349371055637b9858c
81 N106930d2c0e44a34a0cbfda88e5a7e57
82 N5dbcdd6b91f64511bbde34e9ecee50ad
83 schema:sameAs https://app.dimensions.ai/details/publication/pub.1050023144
84 https://doi.org/10.1038/1811212a0
85 schema:sdDatePublished 2022-06-01T22:13
86 schema:sdLicense https://scigraph.springernature.com/explorer/license/
87 schema:sdPublisher N6401400089824475a78abc144d929548
88 schema:url https://doi.org/10.1038/1811212a0
89 sgo:license sg:explorer/license/
90 sgo:sdDataset articles
91 rdf:type schema:ScholarlyArticle
92 N091cf1ebee364d349371055637b9858c schema:name pubmed_id
93 schema:value 13541432
94 rdf:type schema:PropertyValue
95 N106930d2c0e44a34a0cbfda88e5a7e57 schema:name dimensions_id
96 schema:value pub.1050023144
97 rdf:type schema:PropertyValue
98 N4489039e4d814a7abc8ab0a563bbdcc4 schema:volumeNumber 181
99 rdf:type schema:PublicationVolume
100 N5b0a4aab53744621ad93501f9763d071 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
101 schema:name In Vitro Techniques
102 rdf:type schema:DefinedTerm
103 N5dbcdd6b91f64511bbde34e9ecee50ad schema:name doi
104 schema:value 10.1038/1811212a0
105 rdf:type schema:PropertyValue
106 N5f52a1cb01ce44cfbe18b79be952ac35 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
107 schema:name Host Specificity
108 rdf:type schema:DefinedTerm
109 N6401400089824475a78abc144d929548 schema:name Springer Nature - SN SciGraph project
110 rdf:type schema:Organization
111 N86fd9bbe2ce4418894c575683c3f557f schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
112 schema:name Ethyl Methanesulfonate
113 rdf:type schema:DefinedTerm
114 N94d18b861ad64658ad9ff5a6da3090ef schema:issueNumber 4617
115 rdf:type schema:PublicationIssue
116 Na1cf3ede00ff44d7a67dab14aa388944 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
117 schema:name Methane
118 rdf:type schema:DefinedTerm
119 Nb5949cc48de044a2ac216822f42cd3ce schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
120 schema:name Bacteriophages
121 rdf:type schema:DefinedTerm
122 Nec22d6f3c02449d3ace1eba03ed01de4 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
123 schema:name Mutation
124 rdf:type schema:DefinedTerm
125 Neeaafe7e003044bf849214bb31989137 schema:affiliation grid-institutes:grid.424926.f
126 schema:familyName LOVELESS
127 schema:givenName ANTHONY
128 rdf:type schema:Person
129 Nf62ebf8b6bd741a8a59c6b535b871994 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
130 schema:name Sulfonic Acids
131 rdf:type schema:DefinedTerm
132 Nfe12ba0f23964012ab50552a56f5c86c rdf:first Neeaafe7e003044bf849214bb31989137
133 rdf:rest rdf:nil
134 Nff3ee506dec24bdd8e52f82bb583d7b2 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
135 schema:name Plaque, Amyloid
136 rdf:type schema:DefinedTerm
137 anzsrc-for:11 schema:inDefinedTermSet anzsrc-for:
138 schema:name Medical and Health Sciences
139 rdf:type schema:DefinedTerm
140 anzsrc-for:1108 schema:inDefinedTermSet anzsrc-for:
141 schema:name Medical Microbiology
142 rdf:type schema:DefinedTerm
143 sg:journal.1018957 schema:issn 0028-0836
144 1476-4687
145 schema:name Nature
146 schema:publisher Springer Nature
147 rdf:type schema:Periodical
148 sg:pub.10.1038/172964a0 schema:sameAs https://app.dimensions.ai/details/publication/pub.1046643116
149 https://doi.org/10.1038/172964a0
150 rdf:type schema:CreativeWork
151 sg:pub.10.1038/178529b0 schema:sameAs https://app.dimensions.ai/details/publication/pub.1030249739
152 https://doi.org/10.1038/178529b0
153 rdf:type schema:CreativeWork
154 grid-institutes:grid.424926.f schema:alternateName Chester Beatty Research Institute, Institute of Cancer Research, Royal Cancer Hospital, Fulham Road, S.W.3, London
155 schema:name Chester Beatty Research Institute, Institute of Cancer Research, Royal Cancer Hospital, Fulham Road, S.W.3, London
156 rdf:type schema:Organization
 




Preview window. Press ESC to close (or click here)


...