Intracellular Distribution of Choline Acetylase View Full Text


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Article Info

DATE

1956-08

AUTHORS

CATHERINE O. HEBB, B. N. SMALLMAN

ABSTRACT

WITH the development of methods for the measurement of choline acetylase independently of the enzymic formation of acetyl-coenzyme A1–3, it has become possible to determine the intracellular distribution of the enzyme. In the present experiments, rabbit brain homogenates in 0.25 M sucrose were fractionated by differential centrifugation. In exp. 1 the procedure of Brody and Bain4 was followed; in exp. 2, somewhat different centrifugal forces were applied as shown in Table 1. Each particulate fraction was washed by resuspension in 0.25 M sucrose and recentrifugation at the same speed; the washings were then pooled with the original supernatant for the next stage of the centrifugation. More... »

PAGES

365-366

Identifiers

URI

http://scigraph.springernature.com/pub.10.1038/178365b0

DOI

http://dx.doi.org/10.1038/178365b0

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1034860971

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/13358748


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