Reaction of Penicillin with the Staphylococcal Cell Wall View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

1952-02

AUTHORS

A. V. FEW, P. D. COOPER, D. ROWLEY

ABSTRACT

IT has been demonstrated previously1–3 that contact between resting sensitive staphylococci and penicillin results in a small firmly-bound uptake of the drug by the bacteria. When washed suspensions of Staphylococcus aureus with radioactive penicillin attached were ruptured by shaking with fine glass beads, subsequent radiometric assay of the deposit and supernatant after centrifuging suggested that the penicillin had been concentrated within the cytoplasm of the cell4. However, when unlabelled organisms were disrupted by the same method, it was found in a large number of experiments that while very little, if any, of the penicillin-binding capacity of the intact cell could be detected in the non-dialysable fraction of the cytoplasm, a larger percentage was frequently found in the cell-wall fraction than could be accounted for by the few intact bacteria present. More... »

PAGES

283-284

Identifiers

URI

http://scigraph.springernature.com/pub.10.1038/169283a0

DOI

http://dx.doi.org/10.1038/169283a0

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1037760203

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/14910755


Indexing Status Check whether this publication has been indexed by Scopus and Web Of Science using the SN Indexing Status Tool
Incoming Citations Browse incoming citations for this publication using opencitations.net

JSON-LD is the canonical representation for SciGraph data.

TIP: You can open this SciGraph record using an external JSON-LD service: JSON-LD Playground Google SDTT

[
  {
    "@context": "https://springernature.github.io/scigraph/jsonld/sgcontext.json", 
    "about": [
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/06", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Biological Sciences", 
        "type": "DefinedTerm"
      }, 
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/0601", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Biochemistry and Cell Biology", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Cell Wall", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Micrococcus", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Penicillins", 
        "type": "DefinedTerm"
      }
    ], 
    "author": [
      {
        "affiliation": {
          "alternateName": "Department of Colloid Science, University, Cambridge", 
          "id": "http://www.grid.ac/institutes/None", 
          "name": [
            "Wright\u2013Fleming Institute of Microbiology, St. Mary's Hospital Medical School, W.2, Paddington, London", 
            "Department of Colloid Science, University, Cambridge"
          ], 
          "type": "Organization"
        }, 
        "familyName": "FEW", 
        "givenName": "A. V.", 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Wright\u2013Fleming Institute of Microbiology, St. Mary's Hospital Medical School, W.2, Paddington, London", 
          "id": "http://www.grid.ac/institutes/grid.416538.d", 
          "name": [
            "Wright\u2013Fleming Institute of Microbiology, St. Mary's Hospital Medical School, W.2, Paddington, London"
          ], 
          "type": "Organization"
        }, 
        "familyName": "COOPER", 
        "givenName": "P. D.", 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Wright\u2013Fleming Institute of Microbiology, St. Mary's Hospital Medical School, W.2, Paddington, London", 
          "id": "http://www.grid.ac/institutes/grid.416538.d", 
          "name": [
            "Wright\u2013Fleming Institute of Microbiology, St. Mary's Hospital Medical School, W.2, Paddington, London"
          ], 
          "type": "Organization"
        }, 
        "familyName": "ROWLEY", 
        "givenName": "D.", 
        "type": "Person"
      }
    ], 
    "citation": [
      {
        "id": "sg:pub.10.1038/163480b0", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1017469154", 
          "https://doi.org/10.1038/163480b0"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/166218a0", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1045227430", 
          "https://doi.org/10.1038/166218a0"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/164842a0", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1027360938", 
          "https://doi.org/10.1038/164842a0"
        ], 
        "type": "CreativeWork"
      }
    ], 
    "datePublished": "1952-02", 
    "datePublishedReg": "1952-02-01", 
    "description": "IT has been demonstrated previously1\u20133 that contact between resting sensitive staphylococci and penicillin results in a small firmly-bound uptake of the drug by the bacteria. When washed suspensions of Staphylococcus aureus with radioactive penicillin attached were ruptured by shaking with fine glass beads, subsequent radiometric assay of the deposit and supernatant after centrifuging suggested that the penicillin had been concentrated within the cytoplasm of the cell4. However, when unlabelled organisms were disrupted by the same method, it was found in a large number of experiments that while very little, if any, of the penicillin-binding capacity of the intact cell could be detected in the non-dialysable fraction of the cytoplasm, a larger percentage was frequently found in the cell-wall fraction than could be accounted for by the few intact bacteria present.", 
    "genre": "article", 
    "id": "sg:pub.10.1038/169283a0", 
    "isAccessibleForFree": false, 
    "isPartOf": [
      {
        "id": "sg:journal.1018957", 
        "issn": [
          "0028-0836", 
          "1476-4687"
        ], 
        "name": "Nature", 
        "publisher": "Springer Nature", 
        "type": "Periodical"
      }, 
      {
        "issueNumber": "4294", 
        "type": "PublicationIssue"
      }, 
      {
        "type": "PublicationVolume", 
        "volumeNumber": "169"
      }
    ], 
    "keywords": [
      "penicillin-binding capacity", 
      "cell wall fraction", 
      "staphylococcal cell wall", 
      "cell wall", 
      "intact cells", 
      "intact bacteria", 
      "radioactive penicillin", 
      "cytoplasm", 
      "bacteria", 
      "previously1\u20133", 
      "organisms", 
      "large number", 
      "cells", 
      "Staphylococcus aureus", 
      "uptake", 
      "supernatant", 
      "fraction", 
      "staphylococci", 
      "large percentage", 
      "penicillin", 
      "aureus", 
      "wall", 
      "beads", 
      "number", 
      "experiments", 
      "capacity", 
      "suspension", 
      "contact", 
      "results", 
      "percentage", 
      "drugs", 
      "same method", 
      "glass beads", 
      "reaction", 
      "deposits", 
      "sensitive staphylococci", 
      "method", 
      "reaction of penicillins", 
      "fine glass beads", 
      "non-dialysable fraction"
    ], 
    "name": "Reaction of Penicillin with the Staphylococcal Cell Wall", 
    "pagination": "283-284", 
    "productId": [
      {
        "name": "dimensions_id", 
        "type": "PropertyValue", 
        "value": [
          "pub.1037760203"
        ]
      }, 
      {
        "name": "doi", 
        "type": "PropertyValue", 
        "value": [
          "10.1038/169283a0"
        ]
      }, 
      {
        "name": "pubmed_id", 
        "type": "PropertyValue", 
        "value": [
          "14910755"
        ]
      }
    ], 
    "sameAs": [
      "https://doi.org/10.1038/169283a0", 
      "https://app.dimensions.ai/details/publication/pub.1037760203"
    ], 
    "sdDataset": "articles", 
    "sdDatePublished": "2022-05-20T07:30", 
    "sdLicense": "https://scigraph.springernature.com/explorer/license/", 
    "sdPublisher": {
      "name": "Springer Nature - SN SciGraph project", 
      "type": "Organization"
    }, 
    "sdSource": "s3://com-springernature-scigraph/baseset/20220519/entities/gbq_results/article/article_68.jsonl", 
    "type": "ScholarlyArticle", 
    "url": "https://doi.org/10.1038/169283a0"
  }
]
 

Download the RDF metadata as:  json-ld nt turtle xml License info

HOW TO GET THIS DATA PROGRAMMATICALLY:

JSON-LD is a popular format for linked data which is fully compatible with JSON.

curl -H 'Accept: application/ld+json' 'https://scigraph.springernature.com/pub.10.1038/169283a0'

N-Triples is a line-based linked data format ideal for batch operations.

curl -H 'Accept: application/n-triples' 'https://scigraph.springernature.com/pub.10.1038/169283a0'

Turtle is a human-readable linked data format.

curl -H 'Accept: text/turtle' 'https://scigraph.springernature.com/pub.10.1038/169283a0'

RDF/XML is a standard XML format for linked data.

curl -H 'Accept: application/rdf+xml' 'https://scigraph.springernature.com/pub.10.1038/169283a0'


 

This table displays all metadata directly associated to this object as RDF triples.

140 TRIPLES      21 PREDICATES      72 URIs      61 LITERALS      10 BLANK NODES

Subject Predicate Object
1 sg:pub.10.1038/169283a0 schema:about N78e4d8524f19471fad2c5aa438315c10
2 Nb3cdcdbd4b6e407084665e82a654fc11
3 Ne809995879344895b5641bfe2d88e862
4 anzsrc-for:06
5 anzsrc-for:0601
6 schema:author N0872bf1b33fa42bf9d4851b7fe1735dd
7 schema:citation sg:pub.10.1038/163480b0
8 sg:pub.10.1038/164842a0
9 sg:pub.10.1038/166218a0
10 schema:datePublished 1952-02
11 schema:datePublishedReg 1952-02-01
12 schema:description IT has been demonstrated previously1–3 that contact between resting sensitive staphylococci and penicillin results in a small firmly-bound uptake of the drug by the bacteria. When washed suspensions of Staphylococcus aureus with radioactive penicillin attached were ruptured by shaking with fine glass beads, subsequent radiometric assay of the deposit and supernatant after centrifuging suggested that the penicillin had been concentrated within the cytoplasm of the cell4. However, when unlabelled organisms were disrupted by the same method, it was found in a large number of experiments that while very little, if any, of the penicillin-binding capacity of the intact cell could be detected in the non-dialysable fraction of the cytoplasm, a larger percentage was frequently found in the cell-wall fraction than could be accounted for by the few intact bacteria present.
13 schema:genre article
14 schema:isAccessibleForFree false
15 schema:isPartOf N6ad22d03ed5b401e929bd24ff39655f7
16 Ndfe27194990a4cb29760048d85550068
17 sg:journal.1018957
18 schema:keywords Staphylococcus aureus
19 aureus
20 bacteria
21 beads
22 capacity
23 cell wall
24 cell wall fraction
25 cells
26 contact
27 cytoplasm
28 deposits
29 drugs
30 experiments
31 fine glass beads
32 fraction
33 glass beads
34 intact bacteria
35 intact cells
36 large number
37 large percentage
38 method
39 non-dialysable fraction
40 number
41 organisms
42 penicillin
43 penicillin-binding capacity
44 percentage
45 previously1–3
46 radioactive penicillin
47 reaction
48 reaction of penicillins
49 results
50 same method
51 sensitive staphylococci
52 staphylococcal cell wall
53 staphylococci
54 supernatant
55 suspension
56 uptake
57 wall
58 schema:name Reaction of Penicillin with the Staphylococcal Cell Wall
59 schema:pagination 283-284
60 schema:productId N01bae0633f65493a93cdadb4dd032462
61 N75f26be9691d4880b0fcaa732c69cf1d
62 Ne910603a5cb549e490a5229606582227
63 schema:sameAs https://app.dimensions.ai/details/publication/pub.1037760203
64 https://doi.org/10.1038/169283a0
65 schema:sdDatePublished 2022-05-20T07:30
66 schema:sdLicense https://scigraph.springernature.com/explorer/license/
67 schema:sdPublisher Nd50b9b1e99e3462d92456d7e3d0359b8
68 schema:url https://doi.org/10.1038/169283a0
69 sgo:license sg:explorer/license/
70 sgo:sdDataset articles
71 rdf:type schema:ScholarlyArticle
72 N01411fccce884024960f4078883426f3 schema:affiliation grid-institutes:grid.416538.d
73 schema:familyName ROWLEY
74 schema:givenName D.
75 rdf:type schema:Person
76 N01bae0633f65493a93cdadb4dd032462 schema:name dimensions_id
77 schema:value pub.1037760203
78 rdf:type schema:PropertyValue
79 N0872bf1b33fa42bf9d4851b7fe1735dd rdf:first Nd8b98c72fe74415485606125833ccc84
80 rdf:rest N805cfffaf5934c08b9c6ff8ed7364832
81 N4d99762c24084b37b672f348afd468d0 schema:affiliation grid-institutes:grid.416538.d
82 schema:familyName COOPER
83 schema:givenName P. D.
84 rdf:type schema:Person
85 N6ad22d03ed5b401e929bd24ff39655f7 schema:issueNumber 4294
86 rdf:type schema:PublicationIssue
87 N75f26be9691d4880b0fcaa732c69cf1d schema:name pubmed_id
88 schema:value 14910755
89 rdf:type schema:PropertyValue
90 N78e4d8524f19471fad2c5aa438315c10 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
91 schema:name Micrococcus
92 rdf:type schema:DefinedTerm
93 N805cfffaf5934c08b9c6ff8ed7364832 rdf:first N4d99762c24084b37b672f348afd468d0
94 rdf:rest N820a568092204df4a181158d84679c07
95 N820a568092204df4a181158d84679c07 rdf:first N01411fccce884024960f4078883426f3
96 rdf:rest rdf:nil
97 Nb3cdcdbd4b6e407084665e82a654fc11 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
98 schema:name Penicillins
99 rdf:type schema:DefinedTerm
100 Nd50b9b1e99e3462d92456d7e3d0359b8 schema:name Springer Nature - SN SciGraph project
101 rdf:type schema:Organization
102 Nd8b98c72fe74415485606125833ccc84 schema:affiliation grid-institutes:None
103 schema:familyName FEW
104 schema:givenName A. V.
105 rdf:type schema:Person
106 Ndfe27194990a4cb29760048d85550068 schema:volumeNumber 169
107 rdf:type schema:PublicationVolume
108 Ne809995879344895b5641bfe2d88e862 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
109 schema:name Cell Wall
110 rdf:type schema:DefinedTerm
111 Ne910603a5cb549e490a5229606582227 schema:name doi
112 schema:value 10.1038/169283a0
113 rdf:type schema:PropertyValue
114 anzsrc-for:06 schema:inDefinedTermSet anzsrc-for:
115 schema:name Biological Sciences
116 rdf:type schema:DefinedTerm
117 anzsrc-for:0601 schema:inDefinedTermSet anzsrc-for:
118 schema:name Biochemistry and Cell Biology
119 rdf:type schema:DefinedTerm
120 sg:journal.1018957 schema:issn 0028-0836
121 1476-4687
122 schema:name Nature
123 schema:publisher Springer Nature
124 rdf:type schema:Periodical
125 sg:pub.10.1038/163480b0 schema:sameAs https://app.dimensions.ai/details/publication/pub.1017469154
126 https://doi.org/10.1038/163480b0
127 rdf:type schema:CreativeWork
128 sg:pub.10.1038/164842a0 schema:sameAs https://app.dimensions.ai/details/publication/pub.1027360938
129 https://doi.org/10.1038/164842a0
130 rdf:type schema:CreativeWork
131 sg:pub.10.1038/166218a0 schema:sameAs https://app.dimensions.ai/details/publication/pub.1045227430
132 https://doi.org/10.1038/166218a0
133 rdf:type schema:CreativeWork
134 grid-institutes:None schema:alternateName Department of Colloid Science, University, Cambridge
135 schema:name Department of Colloid Science, University, Cambridge
136 Wright–Fleming Institute of Microbiology, St. Mary's Hospital Medical School, W.2, Paddington, London
137 rdf:type schema:Organization
138 grid-institutes:grid.416538.d schema:alternateName Wright–Fleming Institute of Microbiology, St. Mary's Hospital Medical School, W.2, Paddington, London
139 schema:name Wright–Fleming Institute of Microbiology, St. Mary's Hospital Medical School, W.2, Paddington, London
140 rdf:type schema:Organization
 




Preview window. Press ESC to close (or click here)


...