A generic protein purification method for protein complex characterization and proteome exploration View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

1999-10

AUTHORS

Guillaume Rigaut, Anna Shevchenko, Berthold Rutz, Matthias Wilm, Matthias Mann, Bertrand Séraphin

ABSTRACT

We have developed a generic procedure to purify proteins expressed at their natural level under native conditions using a novel tandem affinity purification (TAP) tag. The TAP tag allows the rapid purification of complexes from a relatively small number of cells without prior knowledge of the complex composition, activity, or function. Combined with mass spectrometry, the TAP strategy allows for the identification of proteins interacting with a given target protein. The TAP method has been tested in yeast but should be applicable to other cells or organisms. More... »

PAGES

1030

Journal

TITLE

Nature Biotechnology

ISSUE

10

VOLUME

17

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  • Identifiers

    URI

    http://scigraph.springernature.com/pub.10.1038/13732

    DOI

    http://dx.doi.org/10.1038/13732

    DIMENSIONS

    https://app.dimensions.ai/details/publication/pub.1045828427

    PUBMED

    https://www.ncbi.nlm.nih.gov/pubmed/10504710


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