A method for rapid gain-of-function studies in the mouse embryonic nervous system View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

1999-09

AUTHORS

N Gaiano, J D Kohtz, D H Turnbull, G Fishell

ABSTRACT

We used ultrasound image-guided injections of high-titer retroviral vectors to obtain widespread introduction of genes into the mouse nervous system in utero as early as embryonic day 8.5 (E8.5). The vectors used included internal promoters that substantially improved proviral gene expression in the ventricular zone of the brain. To demonstrate the utility of this system, we extended our previous work in vitro by infecting the telencephalon in vivo as early as E8. 5 with a virus expressing Sonic Hedgehog. Infected embryos showed gross morphological brain defects, as well as ectopic expression of ventral telencephalic markers characteristic of either the medial or lateral ganglionic eminences. More... »

PAGES

812-819

Identifiers

URI

http://scigraph.springernature.com/pub.10.1038/12186

DOI

http://dx.doi.org/10.1038/12186

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1027048246

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/10461220


Indexing Status Check whether this publication has been indexed by Scopus and Web Of Science using the SN Indexing Status Tool
Incoming Citations Browse incoming citations for this publication using opencitations.net

JSON-LD is the canonical representation for SciGraph data.

TIP: You can open this SciGraph record using an external JSON-LD service: JSON-LD Playground Google SDTT

[
  {
    "@context": "https://springernature.github.io/scigraph/jsonld/sgcontext.json", 
    "about": [
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/0604", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Genetics", 
        "type": "DefinedTerm"
      }, 
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/06", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Biological Sciences", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Abnormalities, Multiple", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Animals", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Body Patterning", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Embryo, Mammalian", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Embryonic Induction", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Female", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Genetic Vectors", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Hedgehog Proteins", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Mice", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Mice, Transgenic", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Nervous System", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Organ Specificity", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Pregnancy", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Promoter Regions, Genetic", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Proteins", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Retroviridae", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Trans-Activators", 
        "type": "DefinedTerm"
      }
    ], 
    "author": [
      {
        "affiliation": {
          "alternateName": "New York University", 
          "id": "https://www.grid.ac/institutes/grid.137628.9", 
          "name": [
            "[1] Developmental Genetics Program and the Department of Cell Biology, New York University School of Medicine, 540 First Avenue, New York, New York 10016, USA."
          ], 
          "type": "Organization"
        }, 
        "familyName": "Gaiano", 
        "givenName": "N", 
        "id": "sg:person.01050401001.88", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01050401001.88"
        ], 
        "type": "Person"
      }, 
      {
        "familyName": "Kohtz", 
        "givenName": "J D", 
        "id": "sg:person.01310574246.11", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01310574246.11"
        ], 
        "type": "Person"
      }, 
      {
        "familyName": "Turnbull", 
        "givenName": "D H", 
        "id": "sg:person.0601452276.13", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0601452276.13"
        ], 
        "type": "Person"
      }, 
      {
        "familyName": "Fishell", 
        "givenName": "G", 
        "id": "sg:person.014260463677.90", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.014260463677.90"
        ], 
        "type": "Person"
      }
    ], 
    "citation": [
      {
        "id": "https://doi.org/10.1016/0092-8674(95)90536-7", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1000667307"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/0378-1119(94)90070-1", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1001955448"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/0378-1119(94)90070-1", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1001955448"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/s0896-6273(00)80044-6", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1002687085"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/s0091-679x(08)60600-7", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1003456629"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/s0896-6273(00)80937-x", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1005054633"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1002/cne.901530104", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1007403392"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1002/cne.901530104", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1007403392"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/0092-8674(93)90627-3", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1010498573"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/328131a0", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1010770970", 
          "https://doi.org/10.1038/328131a0"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/0896-6273(92)90216-z", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1013420654"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/362638a0", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1014190751", 
          "https://doi.org/10.1038/362638a0"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1006/dbio.1996.0090", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1014800916"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1073/pnas.84.1.156", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1016982563"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/s0092-8674(00)80956-3", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1019939570"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/0092-8674(94)90514-2", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1020714992"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/0378-1119(91)90434-d", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1021517814"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/0378-1119(91)90434-d", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1021517814"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/ng0393-219", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1024908449", 
          "https://doi.org/10.1038/ng0393-219"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1006/bbrc.1996.5882", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1025330442"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/s0092-8674(00)80325-6", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1026177112"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/383407a0", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1026302288", 
          "https://doi.org/10.1038/383407a0"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/0304-419x(96)00017-0", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1028500289"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/s0070-2153(08)60495-0", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1029391281"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/0092-8674(89)90134-7", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1029532758"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/s0959-4388(99)80017-8", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1032701744"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1002/(sici)1097-0177(199812)213:4<370::aid-aja3>3.0.co;2-o", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1033086021"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1073/pnas.92.25.11323", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1035719887"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/s0925-4773(98)00090-2", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1036271342"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/bf02740819", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1036745859", 
          "https://doi.org/10.1007/bf02740819"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/bf02740819", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1036745859", 
          "https://doi.org/10.1007/bf02740819"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1006/viro.1993.1177", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1040075377"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/s0896-6273(00)80959-9", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1040632560"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/0092-8674(85)90109-6", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1040834013"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1006/excr.1998.4175", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1045755443"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/0925-4773(93)90071-5", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1046604095"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/0925-4773(93)90071-5", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1046604095"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/s0960-9822(94)00027-8", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1047618690"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/sj.gt.3300395", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1048354450", 
          "https://doi.org/10.1038/sj.gt.3300395"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/sj.gt.3300395", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1048354450", 
          "https://doi.org/10.1038/sj.gt.3300395"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/s0092-8674(00)80439-0", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1051102871"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/bf00267823", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1052611363", 
          "https://doi.org/10.1007/bf00267823"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/bf00267823", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1052611363", 
          "https://doi.org/10.1007/bf00267823"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1126/science.1734520", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1062503541"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://app.dimensions.ai/details/publication/pub.1074251167", 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://app.dimensions.ai/details/publication/pub.1076689940", 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://app.dimensions.ai/details/publication/pub.1077554031", 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://app.dimensions.ai/details/publication/pub.1078999371", 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1002/j.1460-2075.1986.tb04620.x", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1079556189"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://app.dimensions.ai/details/publication/pub.1081679285", 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1002/j.1460-2075.1994.tb06448.x", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1082561105"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1002/j.1460-2075.1993.tb05858.x", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1082829529"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://app.dimensions.ai/details/publication/pub.1082943978", 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1523/jneurosci.16-19-06183.1996", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1082963680"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1523/jneurosci.17-04-01425.1997", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1083036615"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://app.dimensions.ai/details/publication/pub.1083118360", 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://app.dimensions.ai/details/publication/pub.1083345292", 
        "type": "CreativeWork"
      }
    ], 
    "datePublished": "1999-09", 
    "datePublishedReg": "1999-09-01", 
    "description": "We used ultrasound image-guided injections of high-titer retroviral vectors to obtain widespread introduction of genes into the mouse nervous system in utero as early as embryonic day 8.5 (E8.5). The vectors used included internal promoters that substantially improved proviral gene expression in the ventricular zone of the brain. To demonstrate the utility of this system, we extended our previous work in vitro by infecting the telencephalon in vivo as early as E8. 5 with a virus expressing Sonic Hedgehog. Infected embryos showed gross morphological brain defects, as well as ectopic expression of ventral telencephalic markers characteristic of either the medial or lateral ganglionic eminences.", 
    "genre": "research_article", 
    "id": "sg:pub.10.1038/12186", 
    "inLanguage": [
      "en"
    ], 
    "isAccessibleForFree": false, 
    "isFundedItemOf": [
      {
        "id": "sg:grant.2557788", 
        "type": "MonetaryGrant"
      }, 
      {
        "id": "sg:grant.2613498", 
        "type": "MonetaryGrant"
      }, 
      {
        "id": "sg:grant.2559019", 
        "type": "MonetaryGrant"
      }
    ], 
    "isPartOf": [
      {
        "id": "sg:journal.1118362", 
        "issn": [
          "1097-6256", 
          "1546-1726"
        ], 
        "name": "Nature Neuroscience", 
        "type": "Periodical"
      }, 
      {
        "issueNumber": "9", 
        "type": "PublicationIssue"
      }, 
      {
        "type": "PublicationVolume", 
        "volumeNumber": "2"
      }
    ], 
    "name": "A method for rapid gain-of-function studies in the mouse embryonic nervous\nsystem", 
    "pagination": "812-819", 
    "productId": [
      {
        "name": "readcube_id", 
        "type": "PropertyValue", 
        "value": [
          "80da0262707be016c0b202467a2070c7a9759c88eb51c797bef9564c0e537400"
        ]
      }, 
      {
        "name": "pubmed_id", 
        "type": "PropertyValue", 
        "value": [
          "10461220"
        ]
      }, 
      {
        "name": "nlm_unique_id", 
        "type": "PropertyValue", 
        "value": [
          "9809671"
        ]
      }, 
      {
        "name": "doi", 
        "type": "PropertyValue", 
        "value": [
          "10.1038/12186"
        ]
      }, 
      {
        "name": "dimensions_id", 
        "type": "PropertyValue", 
        "value": [
          "pub.1027048246"
        ]
      }
    ], 
    "sameAs": [
      "https://doi.org/10.1038/12186", 
      "https://app.dimensions.ai/details/publication/pub.1027048246"
    ], 
    "sdDataset": "articles", 
    "sdDatePublished": "2019-04-11T12:27", 
    "sdLicense": "https://scigraph.springernature.com/explorer/license/", 
    "sdPublisher": {
      "name": "Springer Nature - SN SciGraph project", 
      "type": "Organization"
    }, 
    "sdSource": "s3://com-uberresearch-data-dimensions-target-20181106-alternative/cleanup/v134/2549eaecd7973599484d7c17b260dba0a4ecb94b/merge/v9/a6c9fde33151104705d4d7ff012ea9563521a3ce/jats-lookup/v90/0000000362_0000000362/records_87119_00000000.jsonl", 
    "type": "ScholarlyArticle", 
    "url": "http://www.nature.com/neuro/journal/v2/n9/full/nn0999_812.html"
  }
]
 

Download the RDF metadata as:  json-ld nt turtle xml License info

HOW TO GET THIS DATA PROGRAMMATICALLY:

JSON-LD is a popular format for linked data which is fully compatible with JSON.

curl -H 'Accept: application/ld+json' 'https://scigraph.springernature.com/pub.10.1038/12186'

N-Triples is a line-based linked data format ideal for batch operations.

curl -H 'Accept: application/n-triples' 'https://scigraph.springernature.com/pub.10.1038/12186'

Turtle is a human-readable linked data format.

curl -H 'Accept: text/turtle' 'https://scigraph.springernature.com/pub.10.1038/12186'

RDF/XML is a standard XML format for linked data.

curl -H 'Accept: application/rdf+xml' 'https://scigraph.springernature.com/pub.10.1038/12186'


 

This table displays all metadata directly associated to this object as RDF triples.

310 TRIPLES      21 PREDICATES      96 URIs      38 LITERALS      26 BLANK NODES

Subject Predicate Object
1 sg:pub.10.1038/12186 schema:about N03ea1cc15e32445ab8ff11fc7a956e08
2 N1b7ddd91a407468aa2f188521cb5a415
3 N26d7a9df39724121bb2f2b6d29cf1cdc
4 N279ca6aacb304369bd00deeb606b8610
5 N3a976e366c0142dfa5251316c397949f
6 N5be85404d83b4c848c2d1795a86b7c7d
7 N603bf754c41c4987b40f26881a544a4e
8 N65167c0dc102469d9dc44935e3adebca
9 N73e364ab1a414849a4a4e28dba22744e
10 N90489b9ff82a4acb88ce47ecb643abe6
11 Na305582956d64219b19018fce420ce73
12 Nb97f5eee4cc04daa9e0b9f606bd474d3
13 Nd114085d5f124ce59878a671901f8446
14 Nd3bcde565c774bf89c28258ec80d8e16
15 Ne9d2a75c250c41e2b6cb50ac83d05c85
16 Nedd3c0ac15eb45bf989e88a01b04588d
17 Nf9c83821c9574fb0979fd2e0d16bf8ff
18 anzsrc-for:06
19 anzsrc-for:0604
20 schema:author Ne22e64dbefa14cb8bae41b132bc85e96
21 schema:citation sg:pub.10.1007/bf00267823
22 sg:pub.10.1007/bf02740819
23 sg:pub.10.1038/328131a0
24 sg:pub.10.1038/362638a0
25 sg:pub.10.1038/383407a0
26 sg:pub.10.1038/ng0393-219
27 sg:pub.10.1038/sj.gt.3300395
28 https://app.dimensions.ai/details/publication/pub.1074251167
29 https://app.dimensions.ai/details/publication/pub.1076689940
30 https://app.dimensions.ai/details/publication/pub.1077554031
31 https://app.dimensions.ai/details/publication/pub.1078999371
32 https://app.dimensions.ai/details/publication/pub.1081679285
33 https://app.dimensions.ai/details/publication/pub.1082943978
34 https://app.dimensions.ai/details/publication/pub.1083118360
35 https://app.dimensions.ai/details/publication/pub.1083345292
36 https://doi.org/10.1002/(sici)1097-0177(199812)213:4<370::aid-aja3>3.0.co;2-o
37 https://doi.org/10.1002/cne.901530104
38 https://doi.org/10.1002/j.1460-2075.1986.tb04620.x
39 https://doi.org/10.1002/j.1460-2075.1993.tb05858.x
40 https://doi.org/10.1002/j.1460-2075.1994.tb06448.x
41 https://doi.org/10.1006/bbrc.1996.5882
42 https://doi.org/10.1006/dbio.1996.0090
43 https://doi.org/10.1006/excr.1998.4175
44 https://doi.org/10.1006/viro.1993.1177
45 https://doi.org/10.1016/0092-8674(85)90109-6
46 https://doi.org/10.1016/0092-8674(89)90134-7
47 https://doi.org/10.1016/0092-8674(93)90627-3
48 https://doi.org/10.1016/0092-8674(94)90514-2
49 https://doi.org/10.1016/0092-8674(95)90536-7
50 https://doi.org/10.1016/0304-419x(96)00017-0
51 https://doi.org/10.1016/0378-1119(91)90434-d
52 https://doi.org/10.1016/0378-1119(94)90070-1
53 https://doi.org/10.1016/0896-6273(92)90216-z
54 https://doi.org/10.1016/0925-4773(93)90071-5
55 https://doi.org/10.1016/s0070-2153(08)60495-0
56 https://doi.org/10.1016/s0091-679x(08)60600-7
57 https://doi.org/10.1016/s0092-8674(00)80325-6
58 https://doi.org/10.1016/s0092-8674(00)80439-0
59 https://doi.org/10.1016/s0092-8674(00)80956-3
60 https://doi.org/10.1016/s0896-6273(00)80044-6
61 https://doi.org/10.1016/s0896-6273(00)80937-x
62 https://doi.org/10.1016/s0896-6273(00)80959-9
63 https://doi.org/10.1016/s0925-4773(98)00090-2
64 https://doi.org/10.1016/s0959-4388(99)80017-8
65 https://doi.org/10.1016/s0960-9822(94)00027-8
66 https://doi.org/10.1073/pnas.84.1.156
67 https://doi.org/10.1073/pnas.92.25.11323
68 https://doi.org/10.1126/science.1734520
69 https://doi.org/10.1523/jneurosci.16-19-06183.1996
70 https://doi.org/10.1523/jneurosci.17-04-01425.1997
71 schema:datePublished 1999-09
72 schema:datePublishedReg 1999-09-01
73 schema:description We used ultrasound image-guided injections of high-titer retroviral vectors to obtain widespread introduction of genes into the mouse nervous system in utero as early as embryonic day 8.5 (E8.5). The vectors used included internal promoters that substantially improved proviral gene expression in the ventricular zone of the brain. To demonstrate the utility of this system, we extended our previous work in vitro by infecting the telencephalon in vivo as early as E8. 5 with a virus expressing Sonic Hedgehog. Infected embryos showed gross morphological brain defects, as well as ectopic expression of ventral telencephalic markers characteristic of either the medial or lateral ganglionic eminences.
74 schema:genre research_article
75 schema:inLanguage en
76 schema:isAccessibleForFree false
77 schema:isPartOf Nfbd8e1eb201b4a3d803be281a50cc818
78 Nfbebc8ac549a43239655f1fc8c262a47
79 sg:journal.1118362
80 schema:name A method for rapid gain-of-function studies in the mouse embryonic nervous system
81 schema:pagination 812-819
82 schema:productId N75b9e94afbe04beab50a2b9567f48a3c
83 N7ec32f73042841399f69aa02254cf40e
84 Nbcee63795f4b4fd0b4ad731c6fdfe2c0
85 Nc05323bbf8e34c45b6d2e659ac36b4f3
86 Nfde197def6404aab9099ccd231255be7
87 schema:sameAs https://app.dimensions.ai/details/publication/pub.1027048246
88 https://doi.org/10.1038/12186
89 schema:sdDatePublished 2019-04-11T12:27
90 schema:sdLicense https://scigraph.springernature.com/explorer/license/
91 schema:sdPublisher N426956caebe34ba098bb45f8b1b1a200
92 schema:url http://www.nature.com/neuro/journal/v2/n9/full/nn0999_812.html
93 sgo:license sg:explorer/license/
94 sgo:sdDataset articles
95 rdf:type schema:ScholarlyArticle
96 N03ea1cc15e32445ab8ff11fc7a956e08 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
97 schema:name Organ Specificity
98 rdf:type schema:DefinedTerm
99 N1b7ddd91a407468aa2f188521cb5a415 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
100 schema:name Nervous System
101 rdf:type schema:DefinedTerm
102 N26d7a9df39724121bb2f2b6d29cf1cdc schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
103 schema:name Genetic Vectors
104 rdf:type schema:DefinedTerm
105 N279ca6aacb304369bd00deeb606b8610 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
106 schema:name Hedgehog Proteins
107 rdf:type schema:DefinedTerm
108 N29b0b2abee9c487f90860f4a49407938 rdf:first sg:person.0601452276.13
109 rdf:rest Nb3bf23af77bb40348bba1ccb758f8634
110 N3a976e366c0142dfa5251316c397949f schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
111 schema:name Embryo, Mammalian
112 rdf:type schema:DefinedTerm
113 N426956caebe34ba098bb45f8b1b1a200 schema:name Springer Nature - SN SciGraph project
114 rdf:type schema:Organization
115 N5be85404d83b4c848c2d1795a86b7c7d schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
116 schema:name Trans-Activators
117 rdf:type schema:DefinedTerm
118 N603bf754c41c4987b40f26881a544a4e schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
119 schema:name Mice, Transgenic
120 rdf:type schema:DefinedTerm
121 N65167c0dc102469d9dc44935e3adebca schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
122 schema:name Body Patterning
123 rdf:type schema:DefinedTerm
124 N73e364ab1a414849a4a4e28dba22744e schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
125 schema:name Mice
126 rdf:type schema:DefinedTerm
127 N75b9e94afbe04beab50a2b9567f48a3c schema:name doi
128 schema:value 10.1038/12186
129 rdf:type schema:PropertyValue
130 N7ec32f73042841399f69aa02254cf40e schema:name nlm_unique_id
131 schema:value 9809671
132 rdf:type schema:PropertyValue
133 N90489b9ff82a4acb88ce47ecb643abe6 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
134 schema:name Female
135 rdf:type schema:DefinedTerm
136 Na305582956d64219b19018fce420ce73 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
137 schema:name Abnormalities, Multiple
138 rdf:type schema:DefinedTerm
139 Na5d714978de24f00868fb7b32497b567 rdf:first sg:person.01310574246.11
140 rdf:rest N29b0b2abee9c487f90860f4a49407938
141 Nb3bf23af77bb40348bba1ccb758f8634 rdf:first sg:person.014260463677.90
142 rdf:rest rdf:nil
143 Nb97f5eee4cc04daa9e0b9f606bd474d3 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
144 schema:name Animals
145 rdf:type schema:DefinedTerm
146 Nbcee63795f4b4fd0b4ad731c6fdfe2c0 schema:name dimensions_id
147 schema:value pub.1027048246
148 rdf:type schema:PropertyValue
149 Nc05323bbf8e34c45b6d2e659ac36b4f3 schema:name readcube_id
150 schema:value 80da0262707be016c0b202467a2070c7a9759c88eb51c797bef9564c0e537400
151 rdf:type schema:PropertyValue
152 Nd114085d5f124ce59878a671901f8446 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
153 schema:name Proteins
154 rdf:type schema:DefinedTerm
155 Nd3bcde565c774bf89c28258ec80d8e16 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
156 schema:name Promoter Regions, Genetic
157 rdf:type schema:DefinedTerm
158 Ne22e64dbefa14cb8bae41b132bc85e96 rdf:first sg:person.01050401001.88
159 rdf:rest Na5d714978de24f00868fb7b32497b567
160 Ne9d2a75c250c41e2b6cb50ac83d05c85 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
161 schema:name Embryonic Induction
162 rdf:type schema:DefinedTerm
163 Nedd3c0ac15eb45bf989e88a01b04588d schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
164 schema:name Pregnancy
165 rdf:type schema:DefinedTerm
166 Nf9c83821c9574fb0979fd2e0d16bf8ff schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
167 schema:name Retroviridae
168 rdf:type schema:DefinedTerm
169 Nfbd8e1eb201b4a3d803be281a50cc818 schema:volumeNumber 2
170 rdf:type schema:PublicationVolume
171 Nfbebc8ac549a43239655f1fc8c262a47 schema:issueNumber 9
172 rdf:type schema:PublicationIssue
173 Nfde197def6404aab9099ccd231255be7 schema:name pubmed_id
174 schema:value 10461220
175 rdf:type schema:PropertyValue
176 anzsrc-for:06 schema:inDefinedTermSet anzsrc-for:
177 schema:name Biological Sciences
178 rdf:type schema:DefinedTerm
179 anzsrc-for:0604 schema:inDefinedTermSet anzsrc-for:
180 schema:name Genetics
181 rdf:type schema:DefinedTerm
182 sg:grant.2557788 http://pending.schema.org/fundedItem sg:pub.10.1038/12186
183 rdf:type schema:MonetaryGrant
184 sg:grant.2559019 http://pending.schema.org/fundedItem sg:pub.10.1038/12186
185 rdf:type schema:MonetaryGrant
186 sg:grant.2613498 http://pending.schema.org/fundedItem sg:pub.10.1038/12186
187 rdf:type schema:MonetaryGrant
188 sg:journal.1118362 schema:issn 1097-6256
189 1546-1726
190 schema:name Nature Neuroscience
191 rdf:type schema:Periodical
192 sg:person.01050401001.88 schema:affiliation https://www.grid.ac/institutes/grid.137628.9
193 schema:familyName Gaiano
194 schema:givenName N
195 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01050401001.88
196 rdf:type schema:Person
197 sg:person.01310574246.11 schema:familyName Kohtz
198 schema:givenName J D
199 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01310574246.11
200 rdf:type schema:Person
201 sg:person.014260463677.90 schema:familyName Fishell
202 schema:givenName G
203 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.014260463677.90
204 rdf:type schema:Person
205 sg:person.0601452276.13 schema:familyName Turnbull
206 schema:givenName D H
207 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0601452276.13
208 rdf:type schema:Person
209 sg:pub.10.1007/bf00267823 schema:sameAs https://app.dimensions.ai/details/publication/pub.1052611363
210 https://doi.org/10.1007/bf00267823
211 rdf:type schema:CreativeWork
212 sg:pub.10.1007/bf02740819 schema:sameAs https://app.dimensions.ai/details/publication/pub.1036745859
213 https://doi.org/10.1007/bf02740819
214 rdf:type schema:CreativeWork
215 sg:pub.10.1038/328131a0 schema:sameAs https://app.dimensions.ai/details/publication/pub.1010770970
216 https://doi.org/10.1038/328131a0
217 rdf:type schema:CreativeWork
218 sg:pub.10.1038/362638a0 schema:sameAs https://app.dimensions.ai/details/publication/pub.1014190751
219 https://doi.org/10.1038/362638a0
220 rdf:type schema:CreativeWork
221 sg:pub.10.1038/383407a0 schema:sameAs https://app.dimensions.ai/details/publication/pub.1026302288
222 https://doi.org/10.1038/383407a0
223 rdf:type schema:CreativeWork
224 sg:pub.10.1038/ng0393-219 schema:sameAs https://app.dimensions.ai/details/publication/pub.1024908449
225 https://doi.org/10.1038/ng0393-219
226 rdf:type schema:CreativeWork
227 sg:pub.10.1038/sj.gt.3300395 schema:sameAs https://app.dimensions.ai/details/publication/pub.1048354450
228 https://doi.org/10.1038/sj.gt.3300395
229 rdf:type schema:CreativeWork
230 https://app.dimensions.ai/details/publication/pub.1074251167 schema:CreativeWork
231 https://app.dimensions.ai/details/publication/pub.1076689940 schema:CreativeWork
232 https://app.dimensions.ai/details/publication/pub.1077554031 schema:CreativeWork
233 https://app.dimensions.ai/details/publication/pub.1078999371 schema:CreativeWork
234 https://app.dimensions.ai/details/publication/pub.1081679285 schema:CreativeWork
235 https://app.dimensions.ai/details/publication/pub.1082943978 schema:CreativeWork
236 https://app.dimensions.ai/details/publication/pub.1083118360 schema:CreativeWork
237 https://app.dimensions.ai/details/publication/pub.1083345292 schema:CreativeWork
238 https://doi.org/10.1002/(sici)1097-0177(199812)213:4<370::aid-aja3>3.0.co;2-o schema:sameAs https://app.dimensions.ai/details/publication/pub.1033086021
239 rdf:type schema:CreativeWork
240 https://doi.org/10.1002/cne.901530104 schema:sameAs https://app.dimensions.ai/details/publication/pub.1007403392
241 rdf:type schema:CreativeWork
242 https://doi.org/10.1002/j.1460-2075.1986.tb04620.x schema:sameAs https://app.dimensions.ai/details/publication/pub.1079556189
243 rdf:type schema:CreativeWork
244 https://doi.org/10.1002/j.1460-2075.1993.tb05858.x schema:sameAs https://app.dimensions.ai/details/publication/pub.1082829529
245 rdf:type schema:CreativeWork
246 https://doi.org/10.1002/j.1460-2075.1994.tb06448.x schema:sameAs https://app.dimensions.ai/details/publication/pub.1082561105
247 rdf:type schema:CreativeWork
248 https://doi.org/10.1006/bbrc.1996.5882 schema:sameAs https://app.dimensions.ai/details/publication/pub.1025330442
249 rdf:type schema:CreativeWork
250 https://doi.org/10.1006/dbio.1996.0090 schema:sameAs https://app.dimensions.ai/details/publication/pub.1014800916
251 rdf:type schema:CreativeWork
252 https://doi.org/10.1006/excr.1998.4175 schema:sameAs https://app.dimensions.ai/details/publication/pub.1045755443
253 rdf:type schema:CreativeWork
254 https://doi.org/10.1006/viro.1993.1177 schema:sameAs https://app.dimensions.ai/details/publication/pub.1040075377
255 rdf:type schema:CreativeWork
256 https://doi.org/10.1016/0092-8674(85)90109-6 schema:sameAs https://app.dimensions.ai/details/publication/pub.1040834013
257 rdf:type schema:CreativeWork
258 https://doi.org/10.1016/0092-8674(89)90134-7 schema:sameAs https://app.dimensions.ai/details/publication/pub.1029532758
259 rdf:type schema:CreativeWork
260 https://doi.org/10.1016/0092-8674(93)90627-3 schema:sameAs https://app.dimensions.ai/details/publication/pub.1010498573
261 rdf:type schema:CreativeWork
262 https://doi.org/10.1016/0092-8674(94)90514-2 schema:sameAs https://app.dimensions.ai/details/publication/pub.1020714992
263 rdf:type schema:CreativeWork
264 https://doi.org/10.1016/0092-8674(95)90536-7 schema:sameAs https://app.dimensions.ai/details/publication/pub.1000667307
265 rdf:type schema:CreativeWork
266 https://doi.org/10.1016/0304-419x(96)00017-0 schema:sameAs https://app.dimensions.ai/details/publication/pub.1028500289
267 rdf:type schema:CreativeWork
268 https://doi.org/10.1016/0378-1119(91)90434-d schema:sameAs https://app.dimensions.ai/details/publication/pub.1021517814
269 rdf:type schema:CreativeWork
270 https://doi.org/10.1016/0378-1119(94)90070-1 schema:sameAs https://app.dimensions.ai/details/publication/pub.1001955448
271 rdf:type schema:CreativeWork
272 https://doi.org/10.1016/0896-6273(92)90216-z schema:sameAs https://app.dimensions.ai/details/publication/pub.1013420654
273 rdf:type schema:CreativeWork
274 https://doi.org/10.1016/0925-4773(93)90071-5 schema:sameAs https://app.dimensions.ai/details/publication/pub.1046604095
275 rdf:type schema:CreativeWork
276 https://doi.org/10.1016/s0070-2153(08)60495-0 schema:sameAs https://app.dimensions.ai/details/publication/pub.1029391281
277 rdf:type schema:CreativeWork
278 https://doi.org/10.1016/s0091-679x(08)60600-7 schema:sameAs https://app.dimensions.ai/details/publication/pub.1003456629
279 rdf:type schema:CreativeWork
280 https://doi.org/10.1016/s0092-8674(00)80325-6 schema:sameAs https://app.dimensions.ai/details/publication/pub.1026177112
281 rdf:type schema:CreativeWork
282 https://doi.org/10.1016/s0092-8674(00)80439-0 schema:sameAs https://app.dimensions.ai/details/publication/pub.1051102871
283 rdf:type schema:CreativeWork
284 https://doi.org/10.1016/s0092-8674(00)80956-3 schema:sameAs https://app.dimensions.ai/details/publication/pub.1019939570
285 rdf:type schema:CreativeWork
286 https://doi.org/10.1016/s0896-6273(00)80044-6 schema:sameAs https://app.dimensions.ai/details/publication/pub.1002687085
287 rdf:type schema:CreativeWork
288 https://doi.org/10.1016/s0896-6273(00)80937-x schema:sameAs https://app.dimensions.ai/details/publication/pub.1005054633
289 rdf:type schema:CreativeWork
290 https://doi.org/10.1016/s0896-6273(00)80959-9 schema:sameAs https://app.dimensions.ai/details/publication/pub.1040632560
291 rdf:type schema:CreativeWork
292 https://doi.org/10.1016/s0925-4773(98)00090-2 schema:sameAs https://app.dimensions.ai/details/publication/pub.1036271342
293 rdf:type schema:CreativeWork
294 https://doi.org/10.1016/s0959-4388(99)80017-8 schema:sameAs https://app.dimensions.ai/details/publication/pub.1032701744
295 rdf:type schema:CreativeWork
296 https://doi.org/10.1016/s0960-9822(94)00027-8 schema:sameAs https://app.dimensions.ai/details/publication/pub.1047618690
297 rdf:type schema:CreativeWork
298 https://doi.org/10.1073/pnas.84.1.156 schema:sameAs https://app.dimensions.ai/details/publication/pub.1016982563
299 rdf:type schema:CreativeWork
300 https://doi.org/10.1073/pnas.92.25.11323 schema:sameAs https://app.dimensions.ai/details/publication/pub.1035719887
301 rdf:type schema:CreativeWork
302 https://doi.org/10.1126/science.1734520 schema:sameAs https://app.dimensions.ai/details/publication/pub.1062503541
303 rdf:type schema:CreativeWork
304 https://doi.org/10.1523/jneurosci.16-19-06183.1996 schema:sameAs https://app.dimensions.ai/details/publication/pub.1082963680
305 rdf:type schema:CreativeWork
306 https://doi.org/10.1523/jneurosci.17-04-01425.1997 schema:sameAs https://app.dimensions.ai/details/publication/pub.1083036615
307 rdf:type schema:CreativeWork
308 https://www.grid.ac/institutes/grid.137628.9 schema:alternateName New York University
309 schema:name [1] Developmental Genetics Program and the Department of Cell Biology, New York University School of Medicine, 540 First Avenue, New York, New York 10016, USA.
310 rdf:type schema:Organization
 




Preview window. Press ESC to close (or click here)


...