Detection of PCR products using self-probing amplicons and fluorescence View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

1999-08

AUTHORS

David Whitcombe, Jane Theaker, Simon P. Guy, Tom Brown, Steve Little

ABSTRACT

Molecular diagnostics is progressing from low-throughput, heterogeneous, mostly manual technologies to higher throughput, closed-tube, and automated methods. Fluorescence is the favored signaling technology for such assays, and a number of techniques rely on energy transfer between a fluorophore and a proximal quencher molecule. In these methods, dual-labeled probes hybridize to an amplicon and changes in the quenching of the fluorophore are detected. We describe a new technology that is simple to use, gives highly specific information, and avoids the major difficulties of the alternative methods. It uses a primer with an integral tail that is used to probe an extension product of the primer. The probing of a target sequence is thereby converted into a unimolecular event, which has substantial benefits in terms of kinetics, thermodynamics, assay design, and probe reliability. More... »

PAGES

804-807

Journal

TITLE

Nature Biotechnology

ISSUE

8

VOLUME

17

Related Patents

  • Methods For Detection And Quantification Of Small Rna
  • Reagents And Methods For Detecting Neisseria Gonorrhoeae
  • Melting Curve Analysis With Exponential Background Subtraction
  • Method Of Synthesizing Nucleic Acid
  • Assays For The Detection Of Sars-Cov-2
  • Systems And Methods For Multiplex Analysis Of Pcr In Real Time
  • Methods And Compositions For Enrichment Of Nucleic Acids In Mixtures Of Highly Homologous Sequences
  • Primers And Methods For The Detection And Discrimination Of Nucleic Acids
  • Self-Hybridizing Multiple Target Nucleic Acid Probes And Methods Of Use
  • Ssb-Polymerase Fusion Proteins
  • Methods, Compositions, And Kits For Detecting Allelic Variants
  • Multiplex Nucleic Acid Reactions
  • Attaching A Stem-Loop Oligonucleotide To A Double Stranded Dna Molecule
  • Systems And Methods For Multiplex Analysis Of Pcr In Real Time
  • Method For Analyzing Target Nucleic Acid, Kit, And Analyzer
  • Nucleic Acid Detection Combining Amplification With Fragmentation
  • Primers For Melting Analysis
  • Method And Kit For Characterizing Microorganisms
  • Methods For Genome Assembly, Haplotype Phasing, And Target Independent Nucleic Acid Detection
  • Multi-Color Time Resolved Fluorophores Based On Macrocyclic Lanthanide Complexes
  • Methods And Compositions For Detecting Bacterial Contamination
  • Primers And Methods For The Detection And Discrimination Of Nucleic Acids
  • Methods And Compositions For Universal Detection Of Nucleic Acids
  • Luminescent 1-Hydroxy-2-Pyridinone Chelates Of Lanthanides
  • Compositions Including A Double Stranded Nucleic Acid Molecule And A Stem-Loop Oligonucleotide
  • Allele Amplification Bias
  • Assays For The Detection Of Sars-Cov-2
  • Methods For Genome Assembly And Haplotype Phasing
  • Polynucleotide Primers For Detecting Pik3ca Mutations
  • Method, Systems And Apparatus For Single Cell Analysis
  • Methods For Genome Assembly And Haplotype Phasing
  • Methods Of Analysis Of Methylation
  • Compositions For Detection And Analysis Of Polynucleotides Using Light Harvesting Multichromophores
  • Amplification Primers With Non-Standard Bases For Increased Reaction Specificity
  • Methods For Genome Assembly And Haplotype Phasing
  • Polynucleotide Primers For Detecting Pik3ca Mutations
  • Method Using A Nonlinear Optical Technique For Detection Of Interactions Involving A Conformational Change
  • Systems And Methods For Multiplex Analysis Of Pcr In Real Time
  • Preparing Single Stranded Nucleotide Sequences Capable Of Forming Stem-Loop Structures; For Use As Tools In Gene Expression Analysis And Amplification
  • Methods For Preparing Amplifiable Dna Molecules
  • Optical Reporter Compositions
  • Biosensors; Cationic Polymers; Peptide Nucleic Acids; Point Mutations; Quantitative Analysis
  • Modified Nucleic Acid Polymers And Methods For Their Production
  • Proximity Assay With Detection Based On Hybridisation Chain Reaction (Hcr)
  • Using Serial Dilution And Cyclic Aplification To Detect Mutant Cancer-Associated Sequences In Healthy Cell Populations
  • Ssb-Polymerase Fusion Proteins
  • High Definition Microdroplet Printer
  • Assays For Resistance To Echinocandin-Class Drugs
  • Compositions And Methods For Detecting Klebsiella Pneumoniae
  • Polynucleotide Primers For Detecting Pik3ca Mutations
  • Primers For Melting Analysis
  • High Definition Microdroplet Printer
  • Solid Support Assay Systems And Methods Utilizing Non-Standard Bases
  • Luminescent Macrocyclic Lanthanide Complexes
  • Use Of Stem-Loop Oligonucleotides In The Preparation Of Nucleic Acid Molecules
  • Kit For Use In The Amplification And Quantification Of Preferential Sequences
  • Methods Of Analysis Of Methylation
  • Multiplex Detection Assay For Influenza And Rsv Viruses
  • Method And System For Synthesizing A Target Polynucleotide Within A Droplet
  • Methods For Rapid Multiplexed Amplification Of Target Nucleic Acids
  • Allele-Specific Amplification Of Nucleic Acids Using Blocking Oligonucleotides For Wild Type Suppression
  • Macrocyclic Hopo Chelators
  • Methodology For Analysis Of Sequence Variations Within The Hcv Ns5b Genomic Region
  • Generation Of Phased Read-Sets For Genome Assembly And Haplotype Phasing
  • Methods For Sequential Dna Amplification And Sequencing
  • Methods, Compositions, And Kits For Rare Allele Detection
  • Primers For Melting Analysis
  • Allele-Specific Amplification Of Nucleic Acids
  • Primers And Methods For The Detection And Discrimination Of Nucleic Acids
  • Soluble Conjugated Polymers
  • Systems And Methods For Multiplex Analysis Of Pcr In Real Time
  • Saggi Per La Rivelazione Di Sars-Cov-2
  • Methods For Forensic Dna Quantitation
  • Methods And Systems For Detecting Biological Components
  • Methods And Devices Comprising Soluble Conjugated Polymers
  • Dual Oligonucleotide Method Of Nucleic Acid Detection
  • Allele Amplification Bias
  • Solid Support Assay Systems And Methods Utilizing Non-Standard Bases
  • Polynucleotides For The Detection Of Escherichia Coli O157:H7 And Escherichia Coli O157:Nm Verotoxin Producers
  • Methods, Compositions, And Kits For Detecting Allelic Variants
  • Nucleic Acid Detection Combining Amplification With Fragmentation
  • Detection Of Nucleic Acid Reactions On Bead Arrays
  • Synthesis Of 2′,3′-Dideoxynucleosides For Automated Dna Synthesis And Pyrophosphorolysis Activated Polymerization
  • Multiplex Amplification And Detection
  • Aromatic Triamide-Lanthanide Complexes
  • Multiplex Nucleic Acid Reactions
  • Dual Oligonucleotide Method Of Nucleic Acid Detection
  • Materials And Methods For Detection Of Nucleic Acids
  • Assays For Resistance To Echinocandin-Class Drugs
  • Nucleic Acid Probe, Method For Designing Nucleic Acid Probe, And Method For Detecting Target Sequence
  • Polynucleotide Primers For Detecting Pik3ca Mutations
  • Tsg Primer Target Detection
  • Methods, Plasmid Vectors And Primers For Assessing Hiv Viral Fitness
  • Digital Amplification
  • Primers And Methods For Nucleic Acid Amplification
  • Primers For Use In The Amplification Of Preferential Nucleotide Sequences
  • Melting Curve Analysis With Exponential Background Subtraction
  • Dual-Purpose Primers And Probes For Providing Enhanced Hybridization Assays By Disruption Of Secondary Structure Formation
  • Benzene Sulfonamide Thiazole And Oxazole Compounds
  • Structures Incorporating Conformationally Flexible Conjugated Polymers And Methods Of Use
  • Benzene Sulfonamide Thiazole And Oxazole Compounds
  • Solid Support Assay Systems And Methods Utilizing Non-Standard Bases
  • Soluble Conjugated Polymers
  • Saggi Per La Rivelazione Di Sars-Cov-2
  • Macrocyclic Hopo Chelators
  • Dual-Purpose Primers And Probes For Providing Enhanced Hybridization Assays By Disruption Of Secondary Structure Formation
  • Multiplex Nucleic Acid Reactions
  • Use Of Products Of Pcr Amplification Carrying Elements Of Secondary Structure To Improve Pcr-Based Nucleic Acid Detection
  • Oligonucleotide Primer Sets, Probes; Kits; Detecting In Test Sample Human Immunodeficiency Virus Type 1 (Hiv-1)
  • Tagging Nucleic Acids For Sequence Assembly
  • Method For Detecting A Periodically Pulsing Operating Parameter
  • Species-Specific, Genus-Specific And Universal Dna Probes And Amplification Primers To Rapidly Detect And Identify Common Bacterial And Fungal Pathogens And Associated Antibiotic Resistance Genes From Clinical Specimens For Diagnosis In Microbiology Laboratories
  • Solid Support Assay Systems And Methods Utilizing Non-Standard Bases
  • Nucleic Acid Probe, Method For Designing Nucleic Acid Probe, And Method For Detecting Target Sequence
  • Isolation Of Cpg Islands By Thermal Segregation And Enzymatic Selection-Amplification Method
  • Nucleic Acid Sequence Assembly
  • Quantitative Helicase Assay
  • Nano-Plasmonic Molecular Probes And Methods Of Use
  • Enterococcus And Fecal Bacteroides For Rapid Water Quality Assessment
  • Reagents And Methods For Detecting Neisseria Gonorrhoeae
  • Methods Of Using Fet Labeled Oligonucleotides That Include A 3′-5′ Exonuclease Resistant Quencher Domain And Compositions For Practicing The Same
  • Methods For Rapid Multiplexed Amplification Of Target Nucleic Acids
  • Using Cyclic Amplification To Propagate Preferential Nucleotide Sequences
  • Probes For Improved Melt Discrimination And Multiplexing In Nucleic Acid Assays
  • Kits Including Stem-Loop Oligonucleotides For Use In Preparing Nucleic Acid Molecules
  • Species-Specific, Genus-Specific And Universal Dna Probes And Amplification Primers To Rapidly Detect And Identify Common Bacterial And Fungal Pathogens And Associated Antibiotic Resistance Genes From Clinical Specimens For Diagnosis In Microbiology Laboratories
  • Methods For Preparing Mutant Plants
  • System And Method To Synthesize A Target Molecule Within A Droplet
  • Methods For Genome Assembly And Haplotype Phasing
  • Geenratin Preferential Single Stranded Nucleotide Sequences; Obtain Nucleotide Sequences, Incubate With Primers, Amplify With Preferential Polymerase, Recover Nucleotide Sequences
  • Use Of G-Clamp For Improved Allele-Specific Pcr
  • Method For Methylation Analysis
  • Amplifying Nucleotide Sequences; Obtain Nucleotide Sequences, Incubate With Primers And Polymerase, Extend Nucleotide Sequences, Recover Sequences
  • Systems And Methods For Multiplex Analysis Of Pcr In Real Time
  • Nucleic Acid Detection Combining Amplification With Fragmentation
  • Attaching One Strand Of A Stem-Loop Oligonucleotide Having An Inverted Repeat And A Loop To A Double Stranded Nucleic Acid Molecule, And Displacing One Strand Of The Oligonucleotide From The Oligonucleotide-Attached Nucleic Acid Molecule By Strand Displacement Or By Nick Translation Polymerization
  • Methods And Systems For Detecting Biological Components
  • Key Probe Compositions And Methods For Polynucleotide Detection
  • Nucleic Acid Sequence Assembly
  • Methods For Genome Assembly And Haplotype Phasing
  • Methods, Compositions, And Kits For Detecting Allelic Variants
  • Methods For Genotyping Mature Cotton Fibers And Textiles
  • Fluorescently-Labeled Sequence-Specific Primer; Using The Universal Primer In Nucleic Acid Amplification, Such As Real-Time Pcr
  • Methods For Detection And Analysis Of Polynucleotide-Binding Protein Interactions
  • Polynucleotide Primers
  • Sequencing Of Nucleic Acids Via Barcoding In Discrete Entities
  • Benzene Sulfonamide Thiazole And Oxazole Compounds
  • Polynucleotide Primers For Detecting Pik3ca Mutations
  • Nano-Plasmonic Molecular Probes And Methods Of Use
  • Materials And Methods For Detection Of Nucleic Acids
  • Low Temperature Linear-After-The-Exponential Polymerase Chain Reactions; Amplicons; Fluorescent Dna Dye; Quantitative Analysis
  • Probes For Improved Melt Discrimination And Multiplexing In Nucleic Acid Assays
  • Detection Probe Acting By Molecular Recognition
  • Methods For Producing And Using Stem-Loop Oligonucleotides
  • Photoinduced Electron Transfer (Pet) Primer For Nucleic Acid Amplification
  • Solid Support Assay Systems And Methods Utilizing Non-Standard Bases
  • Systems And Methods For Multiplex Analysis Of Pcr In Real Time
  • Assays For Fungal Infection
  • Synthesis Of 2′, 3′-Dideoxynucleosides For Automated Dna Synthesis And Pyrophosphorolysis Activated Polymerization
  • Digital Amplification
  • Hydroxy-Isophthalamide Moieties; Antibodies; High Stability, Luminescence; Kits
  • Highly Conserved Tuf Genes And Their Use To Generate Probes And Primers For Detection Of Coagulase-Negative Staphylococcus
  • Methods And Compositions For Detection Of Nucleic Acids Based On Stabilized Oligonucleotide Probe Complexes
  • Replication; Hybridization; Kits
  • Exonuclease Enabled Proximity Extension Assays
  • Primers And Probes For The Detection Of Hiv
  • Pcr-Activated Sorting (Pas)
  • Methods And Articles For Strand-Specific Polynucleotide Detection With Conjugated Polymers
  • Multilayer Polymer Light-Emitting Diodes For Solid State Lighting Applications
  • Materials And Methods For Detection Of Nucleic Acids
  • Primers And Methods For Nucleic Acid Amplification
  • Methods For Genome Assembly And Haplotype Phasing
  • Assays For Fungal Infection
  • Multiplex Allele Detection
  • Methods And Compositions For Universal Detection Of Nucleic Acids
  • Use Of Base-Modified Deoxynucleoside Triphosphates To Improve Nucleic Acid Detection
  • A Method For Providing Dna Fragments Derived From A Remote Sample
  • Multiplex Detection Assay For Influenza And Rsv Viruses
  • Assays For Fungal Infection
  • Enterococcus And Fecal Bacteroides For Rapid Water Quality Assessment
  • Methods And Compositions For The Detection And Quantification Of E. Coli And Enterococcus
  • Methods For Forensic Dna Quantitation
  • Probes For Improved Melt Discrimination And Multiplexing In Nucleic Acid Assays
  • Polynucleotide Primers For Detecting Pik3ca Mutations
  • Nucleic Acid Probe, Method For Designing Nucleic Acid Probe, And Method For Detecting Target Sequence
  • Systems And Methods For Multiplex Analysis Of Pcr In Real Time
  • Using Shed Cellular Material As Evaluative Indicator Of Cell Proliferative Disorders
  • Multiplex Drop-Off Digital Polymerase Chain Reaction Methods
  • Compositions Including A Double Stranded Nucleic Acid Molecule And A Stem-Loop Oligonucleotide
  • Macrocyclic Hopo Chelators
  • Exonuclease Enabled Proximity Extension Assays
  • Cyanine Dyes
  • Multiplex Oligonucleotide Addition And Target Amplification
  • Methods Of Sequencing Nucleic Acids In Mixtures And Compositions Related Thereto
  • High Specificity Primers, Amplification Methods And Kits
  • Polynucleotide Primers For Detecting Pik3ca Mutations
  • Methods, Compositions, And Kits For Detecting Allelic Variants
  • Hyperthermophilic Polymerase Enabled Proximity Extension Assay
  • Materials And Methods For Detection Of Nucleic Acids
  • Methods Of Using Fet Labeled Oligonucleotides That Include A 3′-5′ Exonuclease Resistant Quencher Domain And Compositions For Practicing The Same
  • Use Of Base-Modified Deoxynucleoside Triphosphates To Improve Nucleic Acid Detection
  • Conjugated Polymers Suitable For Strand-Specific Polynucleotide Detection In Homogeneous And Solid State Assays
  • Polynucleotide Primers For Detecting Pik3ca Mutations
  • Assays For The Detection Of Sars-Cov-2
  • Materials And Methods For Detection Of Nucleic Acids
  • Exonuclease Enabled Proximity Extension Assays
  • High Specificity Primers, Amplification Methods And Kits
  • Polynucleotide Primers For Detecting Pik3ca Mutations
  • Methods, Compositions, And Kits For Detecting Allelic Variants
  • Pcr-Activated Sorting (Pas)
  • Macrocyclic Hopo Chelators
  • Cyanine Dyes
  • A Method For Providing Dna Fragments Derived From A Remote Sample
  • Conjugated Polymers For Use In Homogeneous And Solid State Assays
  • Method And Apparatus For Simultaneous Targeted Sequencing Of Dna, Rna And Protein
  • Detection Of Neisseria Gonorrhoeaes
  • Method For Detecting Mutations And/Or Polymorphisms
  • Oligonucleotides
  • Primers And Methods For Nucleic Acid Amplification
  • Determining Which Allele Is Present In A Sample Tot Reduce Non-Specific Hybridization Or Reduce The Impact Of Non-Specific Hybridization On The Assay By Using Oligonucleotides Having At Least On Non-Standard Base (Iscytosine And Isoguanine); Kits
  • Method For Generating Proximity Probes
  • Polypeptides Having Nucleic Acid Binding Activity And Compositions And Methods For Nucleic Acid Amplification
  • Fluorescent Quenching Detecting Reagents And Methods
  • Amplifying The Target In A Reaction Mixture Of A Reporter Oligonucleotide; A Nucleic Acid Polymerase; And A Pair Of Oligonucleotide Primers, Where At Least One Has At Least One Non-Natural Base; Analyzing Mutations; Quantitative Analysis; One Pot Process; Simplification; Efficiency; High Speed; Kinetics
  • Reagents And Methods For Detecting Neisseria Gonorrhoeae
  • High Specificity Primers, Amplification Methods And Kits
  • Recovering Long-Range Linkage Information From Preserved Samples
  • Methods Of Using Fet Labeled Oligonucleotides That Include A 3′→5′ Exonuclease Resistant Quencher Domain And Compositions For Practicing The Same
  • Multiplex Oligonucleotide Addition And Target Amplification
  • Multiplex Allele Detection
  • Assays For Resistance To Echinocandin-Class Drugs
  • Involves Placing A Region, To Which A Primer Capable Of Isothermally Amplifying The Template Nucleic Acid Will Anneal, In A Condition That Ensures Base Pairing, Using An Arbitrary Primer
  • Highly Conserved Genes And Their Use To Generate Probes And Primers For Detection Of Microorganisms
  • Self-Hybridizing Multiple Target Nucleic Acid Probes And Methods Of Use
  • Method For Determining The Presence Or Absence Of Methylation In A Sample
  • Reagents And Methods For Detecting Neisseria Gonorrhoeae
  • Nonlinear Optical Detection Of Molecules Comprising An Unnatural Amino Acid Possessing A Hyperpolarizability
  • Method For Detecting Nucleic Acid Sequences
  • Methods And Articles For Strand-Specific Polynucleotide Detection With Cationic Multichromophores
  • Methods And Devices Utilizing Soluble Conjugated Polymers
  • Compositions, Methods And Kits For Nucleic Acid Synthesis And Amplification
  • Paroxysmal Extreme Pain Disorder Treatment
  • Methods And Compositions For Detection Of Nucleic Acids Based On Stabilized Oligonucleotide Probe Complexes
  • Nucleic Acid Detection Combining Amplification With Fragmentation
  • Reagents And Methods For Detecting Neisseria Gonorrhoeae
  • Novel Method For The Detection Of Pglu-Abeta Peptides
  • Digital Amplification
  • Optical Camera Alignment
  • Methods For Processing And Amplifying Nucleic Acids
  • Apparatus For Replication, Identification And Quantification Of Target Nucleic Acids; Molecular Diagnostics
  • Probes And Primers For Detection Of Bacterial Pathogens And Antibiotic Resistance Genes
  • Multiplexed Methylation Detection Methods
  • Dna-Based Test For Detection Of Annual And Intermediate Ryegrass
  • Detection Of Target Nucleic Acid Variants
  • Methods Of Using Fet Labeled Oligonucleotides That Include A 3′-5′ Exonuclease Resistant Quencher Domain And Compositions For Practicing The Same
  • Methods And Probes For Detecting A Vancomycin Resistance Gene
  • Identifiers

    URI

    http://scigraph.springernature.com/pub.10.1038/11751

    DOI

    http://dx.doi.org/10.1038/11751

    DIMENSIONS

    https://app.dimensions.ai/details/publication/pub.1022921637

    PUBMED

    https://www.ncbi.nlm.nih.gov/pubmed/10429248


    Indexing Status Check whether this publication has been indexed by Scopus and Web Of Science using the SN Indexing Status Tool
    Incoming Citations Browse incoming citations for this publication using opencitations.net

    JSON-LD is the canonical representation for SciGraph data.

    TIP: You can open this SciGraph record using an external JSON-LD service: JSON-LD Playground Google SDTT

    [
      {
        "@context": "https://springernature.github.io/scigraph/jsonld/sgcontext.json", 
        "about": [
          {
            "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/02", 
            "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
            "name": "Physical Sciences", 
            "type": "DefinedTerm"
          }, 
          {
            "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/0299", 
            "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
            "name": "Other Physical Sciences", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "BRCA2 Protein", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Base Sequence", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "DNA Primers", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Fluorescence", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Kinetics", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Molecular Probes", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Neoplasm Proteins", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Polymerase Chain Reaction", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Thermodynamics", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Transcription Factors", 
            "type": "DefinedTerm"
          }
        ], 
        "author": [
          {
            "affiliation": {
              "alternateName": "AstraZeneca Diagnostics, Gadbrook Park, CW9 7RA, Rudheath, Northwich, Cheshire, UK", 
              "id": "http://www.grid.ac/institutes/None", 
              "name": [
                "AstraZeneca Diagnostics, Gadbrook Park, CW9 7RA, Rudheath, Northwich, Cheshire, UK"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Whitcombe", 
            "givenName": "David", 
            "id": "sg:person.01367751441.27", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01367751441.27"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "AstraZeneca Diagnostics, Gadbrook Park, CW9 7RA, Rudheath, Northwich, Cheshire, UK", 
              "id": "http://www.grid.ac/institutes/None", 
              "name": [
                "AstraZeneca Diagnostics, Gadbrook Park, CW9 7RA, Rudheath, Northwich, Cheshire, UK"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Theaker", 
            "givenName": "Jane", 
            "id": "sg:person.01321636241.09", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01321636241.09"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "AstraZeneca Diagnostics, Gadbrook Park, CW9 7RA, Rudheath, Northwich, Cheshire, UK", 
              "id": "http://www.grid.ac/institutes/None", 
              "name": [
                "AstraZeneca Diagnostics, Gadbrook Park, CW9 7RA, Rudheath, Northwich, Cheshire, UK"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Guy", 
            "givenName": "Simon P.", 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "Department of Chemistry, University of Southampton, SO17 1BJ, Highfield, Southampton, UK", 
              "id": "http://www.grid.ac/institutes/grid.5491.9", 
              "name": [
                "Department of Chemistry, University of Southampton, SO17 1BJ, Highfield, Southampton, UK"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Brown", 
            "givenName": "Tom", 
            "id": "sg:person.07744374412.60", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.07744374412.60"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "AstraZeneca Diagnostics, Gadbrook Park, CW9 7RA, Rudheath, Northwich, Cheshire, UK", 
              "id": "http://www.grid.ac/institutes/None", 
              "name": [
                "AstraZeneca Diagnostics, Gadbrook Park, CW9 7RA, Rudheath, Northwich, Cheshire, UK"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Little", 
            "givenName": "Steve", 
            "type": "Person"
          }
        ], 
        "citation": [
          {
            "id": "sg:pub.10.1038/nbt0396-303", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1007118508", 
              "https://doi.org/10.1038/nbt0396-303"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/nbt0198-49", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1027516191", 
              "https://doi.org/10.1038/nbt0198-49"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/ng0495-341", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1032967302", 
              "https://doi.org/10.1038/ng0495-341"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/ng0596-123", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1033316917", 
              "https://doi.org/10.1038/ng0596-123"
            ], 
            "type": "CreativeWork"
          }
        ], 
        "datePublished": "1999-08", 
        "datePublishedReg": "1999-08-01", 
        "description": "Molecular diagnostics is progressing from low-throughput, heterogeneous, mostly manual technologies to higher throughput, closed-tube, and automated methods. Fluorescence is the favored signaling technology for such assays, and a number of techniques rely on energy transfer between a fluorophore and a proximal quencher molecule. In these methods, dual-labeled probes hybridize to an amplicon and changes in the quenching of the fluorophore are detected. We describe a new technology that is simple to use, gives highly specific information, and avoids the major difficulties of the alternative methods. It uses a primer with an integral tail that is used to probe an extension product of the primer. The probing of a target sequence is thereby converted into a unimolecular event, which has substantial benefits in terms of kinetics, thermodynamics, assay design, and probe reliability.", 
        "genre": "article", 
        "id": "sg:pub.10.1038/11751", 
        "isAccessibleForFree": false, 
        "isPartOf": [
          {
            "id": "sg:journal.1115214", 
            "issn": [
              "1087-0156", 
              "1546-1696"
            ], 
            "name": "Nature Biotechnology", 
            "publisher": "Springer Nature", 
            "type": "Periodical"
          }, 
          {
            "issueNumber": "8", 
            "type": "PublicationIssue"
          }, 
          {
            "type": "PublicationVolume", 
            "volumeNumber": "17"
          }
        ], 
        "keywords": [
          "quencher molecules", 
          "terms of kinetics", 
          "unimolecular event", 
          "energy transfer", 
          "fluorophores", 
          "fluorescence", 
          "number of techniques", 
          "molecules", 
          "products", 
          "extension products", 
          "quenching", 
          "high throughput", 
          "molecular diagnostics", 
          "such assays", 
          "kinetics", 
          "thermodynamics", 
          "target sequence", 
          "probe", 
          "transfer", 
          "PCR products", 
          "method", 
          "probing", 
          "alternative method", 
          "technology", 
          "detection", 
          "throughput", 
          "diagnostics", 
          "new technologies", 
          "amplicons", 
          "primers", 
          "probe reliability", 
          "assays", 
          "technique", 
          "tail", 
          "specific information", 
          "design", 
          "major difficulty", 
          "manual technology", 
          "changes", 
          "reliability", 
          "terms", 
          "sequence", 
          "number", 
          "substantial benefits", 
          "information", 
          "benefits", 
          "events", 
          "difficulties"
        ], 
        "name": "Detection of PCR products using self-probing amplicons and fluorescence", 
        "pagination": "804-807", 
        "productId": [
          {
            "name": "dimensions_id", 
            "type": "PropertyValue", 
            "value": [
              "pub.1022921637"
            ]
          }, 
          {
            "name": "doi", 
            "type": "PropertyValue", 
            "value": [
              "10.1038/11751"
            ]
          }, 
          {
            "name": "pubmed_id", 
            "type": "PropertyValue", 
            "value": [
              "10429248"
            ]
          }
        ], 
        "sameAs": [
          "https://doi.org/10.1038/11751", 
          "https://app.dimensions.ai/details/publication/pub.1022921637"
        ], 
        "sdDataset": "articles", 
        "sdDatePublished": "2022-09-02T15:49", 
        "sdLicense": "https://scigraph.springernature.com/explorer/license/", 
        "sdPublisher": {
          "name": "Springer Nature - SN SciGraph project", 
          "type": "Organization"
        }, 
        "sdSource": "s3://com-springernature-scigraph/baseset/20220902/entities/gbq_results/article/article_334.jsonl", 
        "type": "ScholarlyArticle", 
        "url": "https://doi.org/10.1038/11751"
      }
    ]
     

    Download the RDF metadata as:  json-ld nt turtle xml License info

    HOW TO GET THIS DATA PROGRAMMATICALLY:

    JSON-LD is a popular format for linked data which is fully compatible with JSON.

    curl -H 'Accept: application/ld+json' 'https://scigraph.springernature.com/pub.10.1038/11751'

    N-Triples is a line-based linked data format ideal for batch operations.

    curl -H 'Accept: application/n-triples' 'https://scigraph.springernature.com/pub.10.1038/11751'

    Turtle is a human-readable linked data format.

    curl -H 'Accept: text/turtle' 'https://scigraph.springernature.com/pub.10.1038/11751'

    RDF/XML is a standard XML format for linked data.

    curl -H 'Accept: application/rdf+xml' 'https://scigraph.springernature.com/pub.10.1038/11751'


     

    This table displays all metadata directly associated to this object as RDF triples.

    194 TRIPLES      21 PREDICATES      88 URIs      76 LITERALS      17 BLANK NODES

    Subject Predicate Object
    1 sg:pub.10.1038/11751 schema:about N3233869730e9451db18124e224ac6caa
    2 N34ce3723c91646a7a889065b37da89b8
    3 N388b1465c4be4a2fa1c2cbd447253149
    4 N49a7fc0056d240ccba17da896acde592
    5 N67237831acb94da4a77c50526a541b38
    6 N87932f4468d14d069c313512918f605f
    7 N9a614efe82d2439cb81ae09d978b4747
    8 Naaa7bee789bf46d9ad0949700961ec65
    9 Nefff66a0292f4aeab7cc83f3defb1d00
    10 Nfb0f17e772e54721969026e474896570
    11 anzsrc-for:02
    12 anzsrc-for:0299
    13 schema:author Nc4c63958c9e446119c1b2b7901e2cffb
    14 schema:citation sg:pub.10.1038/nbt0198-49
    15 sg:pub.10.1038/nbt0396-303
    16 sg:pub.10.1038/ng0495-341
    17 sg:pub.10.1038/ng0596-123
    18 schema:datePublished 1999-08
    19 schema:datePublishedReg 1999-08-01
    20 schema:description Molecular diagnostics is progressing from low-throughput, heterogeneous, mostly manual technologies to higher throughput, closed-tube, and automated methods. Fluorescence is the favored signaling technology for such assays, and a number of techniques rely on energy transfer between a fluorophore and a proximal quencher molecule. In these methods, dual-labeled probes hybridize to an amplicon and changes in the quenching of the fluorophore are detected. We describe a new technology that is simple to use, gives highly specific information, and avoids the major difficulties of the alternative methods. It uses a primer with an integral tail that is used to probe an extension product of the primer. The probing of a target sequence is thereby converted into a unimolecular event, which has substantial benefits in terms of kinetics, thermodynamics, assay design, and probe reliability.
    21 schema:genre article
    22 schema:isAccessibleForFree false
    23 schema:isPartOf N9f6e5b0519814826a39f996986a6fc08
    24 Naa813c538d014d02a2e95e0d6cd57932
    25 sg:journal.1115214
    26 schema:keywords PCR products
    27 alternative method
    28 amplicons
    29 assays
    30 benefits
    31 changes
    32 design
    33 detection
    34 diagnostics
    35 difficulties
    36 energy transfer
    37 events
    38 extension products
    39 fluorescence
    40 fluorophores
    41 high throughput
    42 information
    43 kinetics
    44 major difficulty
    45 manual technology
    46 method
    47 molecular diagnostics
    48 molecules
    49 new technologies
    50 number
    51 number of techniques
    52 primers
    53 probe
    54 probe reliability
    55 probing
    56 products
    57 quencher molecules
    58 quenching
    59 reliability
    60 sequence
    61 specific information
    62 substantial benefits
    63 such assays
    64 tail
    65 target sequence
    66 technique
    67 technology
    68 terms
    69 terms of kinetics
    70 thermodynamics
    71 throughput
    72 transfer
    73 unimolecular event
    74 schema:name Detection of PCR products using self-probing amplicons and fluorescence
    75 schema:pagination 804-807
    76 schema:productId N508a52b274634fe5ae1158bb9bb06fdf
    77 Nefe2977de6844cd9b195446e8755f5e0
    78 Nf2ea5af0267d48ccb970666b5a7192f4
    79 schema:sameAs https://app.dimensions.ai/details/publication/pub.1022921637
    80 https://doi.org/10.1038/11751
    81 schema:sdDatePublished 2022-09-02T15:49
    82 schema:sdLicense https://scigraph.springernature.com/explorer/license/
    83 schema:sdPublisher N59a43926a36743e4930888a3669642cd
    84 schema:url https://doi.org/10.1038/11751
    85 sgo:license sg:explorer/license/
    86 sgo:sdDataset articles
    87 rdf:type schema:ScholarlyArticle
    88 N3233869730e9451db18124e224ac6caa schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    89 schema:name Base Sequence
    90 rdf:type schema:DefinedTerm
    91 N34ce3723c91646a7a889065b37da89b8 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    92 schema:name Polymerase Chain Reaction
    93 rdf:type schema:DefinedTerm
    94 N388b1465c4be4a2fa1c2cbd447253149 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    95 schema:name BRCA2 Protein
    96 rdf:type schema:DefinedTerm
    97 N49a7fc0056d240ccba17da896acde592 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    98 schema:name Transcription Factors
    99 rdf:type schema:DefinedTerm
    100 N4a18c8f977bc4b63a81ec3f6a03cc07c schema:affiliation grid-institutes:None
    101 schema:familyName Little
    102 schema:givenName Steve
    103 rdf:type schema:Person
    104 N508a52b274634fe5ae1158bb9bb06fdf schema:name pubmed_id
    105 schema:value 10429248
    106 rdf:type schema:PropertyValue
    107 N59a43926a36743e4930888a3669642cd schema:name Springer Nature - SN SciGraph project
    108 rdf:type schema:Organization
    109 N67237831acb94da4a77c50526a541b38 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    110 schema:name Kinetics
    111 rdf:type schema:DefinedTerm
    112 N87932f4468d14d069c313512918f605f schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    113 schema:name Molecular Probes
    114 rdf:type schema:DefinedTerm
    115 N9153da7837e148c5a5b8554b0e30b2c9 rdf:first N4a18c8f977bc4b63a81ec3f6a03cc07c
    116 rdf:rest rdf:nil
    117 N9a614efe82d2439cb81ae09d978b4747 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    118 schema:name DNA Primers
    119 rdf:type schema:DefinedTerm
    120 N9f6e5b0519814826a39f996986a6fc08 schema:issueNumber 8
    121 rdf:type schema:PublicationIssue
    122 Naa813c538d014d02a2e95e0d6cd57932 schema:volumeNumber 17
    123 rdf:type schema:PublicationVolume
    124 Naaa7bee789bf46d9ad0949700961ec65 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    125 schema:name Thermodynamics
    126 rdf:type schema:DefinedTerm
    127 Nb225e855c60940768f67ce76cc4dd352 rdf:first sg:person.07744374412.60
    128 rdf:rest N9153da7837e148c5a5b8554b0e30b2c9
    129 Nc4c63958c9e446119c1b2b7901e2cffb rdf:first sg:person.01367751441.27
    130 rdf:rest Nde05f41a0163420f8bcbff043629c5e9
    131 Ncda65f870a9c42cbb7a4350755cee3ab rdf:first Ne2a8eafb6ef74c1f8f6a678ae7ad3e98
    132 rdf:rest Nb225e855c60940768f67ce76cc4dd352
    133 Nde05f41a0163420f8bcbff043629c5e9 rdf:first sg:person.01321636241.09
    134 rdf:rest Ncda65f870a9c42cbb7a4350755cee3ab
    135 Ne2a8eafb6ef74c1f8f6a678ae7ad3e98 schema:affiliation grid-institutes:None
    136 schema:familyName Guy
    137 schema:givenName Simon P.
    138 rdf:type schema:Person
    139 Nefe2977de6844cd9b195446e8755f5e0 schema:name dimensions_id
    140 schema:value pub.1022921637
    141 rdf:type schema:PropertyValue
    142 Nefff66a0292f4aeab7cc83f3defb1d00 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    143 schema:name Fluorescence
    144 rdf:type schema:DefinedTerm
    145 Nf2ea5af0267d48ccb970666b5a7192f4 schema:name doi
    146 schema:value 10.1038/11751
    147 rdf:type schema:PropertyValue
    148 Nfb0f17e772e54721969026e474896570 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    149 schema:name Neoplasm Proteins
    150 rdf:type schema:DefinedTerm
    151 anzsrc-for:02 schema:inDefinedTermSet anzsrc-for:
    152 schema:name Physical Sciences
    153 rdf:type schema:DefinedTerm
    154 anzsrc-for:0299 schema:inDefinedTermSet anzsrc-for:
    155 schema:name Other Physical Sciences
    156 rdf:type schema:DefinedTerm
    157 sg:journal.1115214 schema:issn 1087-0156
    158 1546-1696
    159 schema:name Nature Biotechnology
    160 schema:publisher Springer Nature
    161 rdf:type schema:Periodical
    162 sg:person.01321636241.09 schema:affiliation grid-institutes:None
    163 schema:familyName Theaker
    164 schema:givenName Jane
    165 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01321636241.09
    166 rdf:type schema:Person
    167 sg:person.01367751441.27 schema:affiliation grid-institutes:None
    168 schema:familyName Whitcombe
    169 schema:givenName David
    170 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01367751441.27
    171 rdf:type schema:Person
    172 sg:person.07744374412.60 schema:affiliation grid-institutes:grid.5491.9
    173 schema:familyName Brown
    174 schema:givenName Tom
    175 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.07744374412.60
    176 rdf:type schema:Person
    177 sg:pub.10.1038/nbt0198-49 schema:sameAs https://app.dimensions.ai/details/publication/pub.1027516191
    178 https://doi.org/10.1038/nbt0198-49
    179 rdf:type schema:CreativeWork
    180 sg:pub.10.1038/nbt0396-303 schema:sameAs https://app.dimensions.ai/details/publication/pub.1007118508
    181 https://doi.org/10.1038/nbt0396-303
    182 rdf:type schema:CreativeWork
    183 sg:pub.10.1038/ng0495-341 schema:sameAs https://app.dimensions.ai/details/publication/pub.1032967302
    184 https://doi.org/10.1038/ng0495-341
    185 rdf:type schema:CreativeWork
    186 sg:pub.10.1038/ng0596-123 schema:sameAs https://app.dimensions.ai/details/publication/pub.1033316917
    187 https://doi.org/10.1038/ng0596-123
    188 rdf:type schema:CreativeWork
    189 grid-institutes:None schema:alternateName AstraZeneca Diagnostics, Gadbrook Park, CW9 7RA, Rudheath, Northwich, Cheshire, UK
    190 schema:name AstraZeneca Diagnostics, Gadbrook Park, CW9 7RA, Rudheath, Northwich, Cheshire, UK
    191 rdf:type schema:Organization
    192 grid-institutes:grid.5491.9 schema:alternateName Department of Chemistry, University of Southampton, SO17 1BJ, Highfield, Southampton, UK
    193 schema:name Department of Chemistry, University of Southampton, SO17 1BJ, Highfield, Southampton, UK
    194 rdf:type schema:Organization
     




    Preview window. Press ESC to close (or click here)


    ...