Direct analysis of protein complexes using mass spectrometry View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

1999-07

AUTHORS

A J Link, J Eng, D M Schieltz, E Carmack, G J Mize, D R Morris, B M Garvik, J R Yates

ABSTRACT

We describe a rapid, sensitive process for comprehensively identifying proteins in macromolecular complexes that uses multidimensional liquid chromatography (LC) and tandem mass spectrometry (MS/MS) to separate and fragment peptides. The SEQUEST algorithm, relying upon translated genomic sequences, infers amino acid sequences from the fragment ions. The method was applied to the Saccharomyces cerevisiae ribosome leading to the identification of a novel protein component of the yeast and human 40S subunit. By offering the ability to identify >100 proteins in a single run, this process enables components in even the largest macromolecular complexes to be analyzed comprehensively. More... »

PAGES

676-682

Journal

TITLE

Nature Biotechnology

ISSUE

7

VOLUME

17

Author Affiliations

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  • Identifiers

    URI

    http://scigraph.springernature.com/pub.10.1038/10890

    DOI

    http://dx.doi.org/10.1038/10890

    DIMENSIONS

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    PUBMED

    https://www.ncbi.nlm.nih.gov/pubmed/10404161


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