Tools of the trade: use of dominant-inhibitory mutants of Ras-family GTPases View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

1999-06

AUTHORS

L A Feig

ABSTRACT

One of the most powerful ways of studying the function of a protein is to specifically block its activity within cells. Over the past decade, dominant-inhibitory proteins have emerged as popular tools with which to accomplish this task; these mutated proteins interfere with the function of their normal cellular counterparts or with proteins that interact with them. This approach has been used extensively in the elucidation of signal-transduction cascades, such as those involving Ras-family proteins. Here I discuss the power and potential pitfalls of using dominant-inhibitory Ras proteins. More... »

PAGES

e25-e27

Identifiers

URI

http://scigraph.springernature.com/pub.10.1038/10018

DOI

http://dx.doi.org/10.1038/10018

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1045007249

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/10559887


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