Macrophage-Specific Overexpression of Human Matrix Metalloproteinase-12 in Transgenic Rabbits View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

2004-06

AUTHORS

Jianglin Fan, Xiaofei Wang, Lihua Wu, Shin-Ich Matsumoto, Jingyan Liang, Tomonari Koike, Tomonaga Ichikawa, Huijun Sun, Hisataka Shikama, Yasuyuki Sasaguri, Teruo Watanabe

ABSTRACT

Increased matrix metalloproteinase-12 (MMP-12) has been implicated in atherosclerosis and many other inflammatory processes. To define MMP-12 functions in vivo, we generated transgenic rabbits that expressed human (h) MMP-12 gene under the control of a macrophage-specific promoter, the human scavenger receptor promoter. Two transgenic founder rabbits were found to have hMMP-12 transgene integration by Southern blot analysis. hMMP-12 mRNA was expressed in peritoneal and alveolar macrophages, and in tissues enriched in macrophages in transgenic rabbits. High levels of hMMP-12 protein were detected in the conditioned media of cultured peritoneal and alveolar macrophages from transgenic rabbits. Zymography showed that hMMP-12 secreted from macrophages possessed enzymatic activity toward β-casein. To evaluate the expression of hMMP-12 in inflammatory sites, we used carrageenan-induced granulomas as an in vivo model for tissue macrophages and foam cells. Granuloma size in transgenic rabbits was significantly increased compared to that in control rabbits, and histological examination revealed that granulomas of transgenic rabbits were enriched in macrophages associated with increased hMMP-12 expression. We believe that this transgenic rabbit model with increased expression of hMMP-12 may become a useful model for further mechanistic studies of MMP-12 in inflammatory diseases and cancer invasion; it is also an ideal model for testing the in vivo action of MMP-12 inhibitors. More... »

PAGES

261-269

Identifiers

URI

http://scigraph.springernature.com/pub.10.1023/b:trag.0000034717.70729.61

DOI

http://dx.doi.org/10.1023/b:trag.0000034717.70729.61

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1003968565

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/15359603


Indexing Status Check whether this publication has been indexed by Scopus and Web Of Science using the SN Indexing Status Tool
Incoming Citations Browse incoming citations for this publication using opencitations.net

JSON-LD is the canonical representation for SciGraph data.

TIP: You can open this SciGraph record using an external JSON-LD service: JSON-LD Playground Google SDTT

[
  {
    "@context": "https://springernature.github.io/scigraph/jsonld/sgcontext.json", 
    "about": [
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/06", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Biological Sciences", 
        "type": "DefinedTerm"
      }, 
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/0601", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Biochemistry and Cell Biology", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Animals", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Animals, Genetically Modified", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Blotting, Southern", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Caseins", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "DNA Primers", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Gene Expression", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Granuloma", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Humans", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Macrophages", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Matrix Metalloproteinase 12", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Metalloendopeptidases", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Models, Animal", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Promoter Regions, Genetic", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Rabbits", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Receptors, Immunologic", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Receptors, Scavenger", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Transformation, Genetic", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Transgenes", 
        "type": "DefinedTerm"
      }
    ], 
    "author": [
      {
        "affiliation": {
          "alternateName": "Laboratory of Cardiovascular Disease, Department of Pathology, Institute of Basic Medical Sciences, University of Tsukuba, 305-8575, Tsukuba, Japan", 
          "id": "http://www.grid.ac/institutes/grid.20515.33", 
          "name": [
            "Laboratory of Cardiovascular Disease, Department of Pathology, Institute of Basic Medical Sciences, University of Tsukuba, 305-8575, Tsukuba, Japan"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Fan", 
        "givenName": "Jianglin", 
        "id": "sg:person.01136672602.49", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01136672602.49"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Laboratory of Cardiovascular Disease, Department of Pathology, Institute of Basic Medical Sciences, University of Tsukuba, 305-8575, Tsukuba, Japan", 
          "id": "http://www.grid.ac/institutes/grid.20515.33", 
          "name": [
            "Laboratory of Cardiovascular Disease, Department of Pathology, Institute of Basic Medical Sciences, University of Tsukuba, 305-8575, Tsukuba, Japan"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Wang", 
        "givenName": "Xiaofei", 
        "id": "sg:person.01300565244.96", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01300565244.96"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Laboratory of Cardiovascular Disease, Department of Pathology, Institute of Basic Medical Sciences, University of Tsukuba, 305-8575, Tsukuba, Japan", 
          "id": "http://www.grid.ac/institutes/grid.20515.33", 
          "name": [
            "Laboratory of Cardiovascular Disease, Department of Pathology, Institute of Basic Medical Sciences, University of Tsukuba, 305-8575, Tsukuba, Japan"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Wu", 
        "givenName": "Lihua", 
        "id": "sg:person.01321234402.12", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01321234402.12"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Yamanouchi Pharmaceutical Company Research Institute, 305-8585, Tsukuba, Japan", 
          "id": "http://www.grid.ac/institutes/None", 
          "name": [
            "Yamanouchi Pharmaceutical Company Research Institute, 305-8585, Tsukuba, Japan"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Matsumoto", 
        "givenName": "Shin-Ich", 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Laboratory of Cardiovascular Disease, Department of Pathology, Institute of Basic Medical Sciences, University of Tsukuba, 305-8575, Tsukuba, Japan", 
          "id": "http://www.grid.ac/institutes/grid.20515.33", 
          "name": [
            "Laboratory of Cardiovascular Disease, Department of Pathology, Institute of Basic Medical Sciences, University of Tsukuba, 305-8575, Tsukuba, Japan"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Liang", 
        "givenName": "Jingyan", 
        "id": "sg:person.01172340432.80", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01172340432.80"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Laboratory of Cardiovascular Disease, Department of Pathology, Institute of Basic Medical Sciences, University of Tsukuba, 305-8575, Tsukuba, Japan", 
          "id": "http://www.grid.ac/institutes/grid.20515.33", 
          "name": [
            "Laboratory of Cardiovascular Disease, Department of Pathology, Institute of Basic Medical Sciences, University of Tsukuba, 305-8575, Tsukuba, Japan"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Koike", 
        "givenName": "Tomonari", 
        "id": "sg:person.011340333222.04", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.011340333222.04"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Laboratory of Cardiovascular Disease, Department of Pathology, Institute of Basic Medical Sciences, University of Tsukuba, 305-8575, Tsukuba, Japan", 
          "id": "http://www.grid.ac/institutes/grid.20515.33", 
          "name": [
            "Laboratory of Cardiovascular Disease, Department of Pathology, Institute of Basic Medical Sciences, University of Tsukuba, 305-8575, Tsukuba, Japan"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Ichikawa", 
        "givenName": "Tomonaga", 
        "id": "sg:person.0625674757.78", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0625674757.78"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Department of Pharmacology, Dalian Medical University, 116027, Dalian, China", 
          "id": "http://www.grid.ac/institutes/grid.411971.b", 
          "name": [
            "Laboratory of Cardiovascular Disease, Department of Pathology, Institute of Basic Medical Sciences, University of Tsukuba, 305-8575, Tsukuba, Japan", 
            "Department of Pharmacology, Dalian Medical University, 116027, Dalian, China"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Sun", 
        "givenName": "Huijun", 
        "id": "sg:person.0762316763.77", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0762316763.77"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Yamanouchi Pharmaceutical Company Research Institute, 305-8585, Tsukuba, Japan", 
          "id": "http://www.grid.ac/institutes/None", 
          "name": [
            "Yamanouchi Pharmaceutical Company Research Institute, 305-8585, Tsukuba, Japan"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Shikama", 
        "givenName": "Hisataka", 
        "id": "sg:person.01054341145.34", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01054341145.34"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Department of Pathology and Cell Biology, School of Medicine, University of Occupational and Environmental Health, 807-8555, Kitakyushu, Japan", 
          "id": "http://www.grid.ac/institutes/grid.271052.3", 
          "name": [
            "Department of Pathology and Cell Biology, School of Medicine, University of Occupational and Environmental Health, 807-8555, Kitakyushu, Japan"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Sasaguri", 
        "givenName": "Yasuyuki", 
        "id": "sg:person.01304023165.50", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01304023165.50"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Saga University, 840-8502, Saga, Japan", 
          "id": "http://www.grid.ac/institutes/grid.412339.e", 
          "name": [
            "Saga University, 840-8502, Saga, Japan"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Watanabe", 
        "givenName": "Teruo", 
        "id": "sg:person.012664624074.29", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.012664624074.29"
        ], 
        "type": "Person"
      }
    ], 
    "citation": [
      {
        "id": "sg:pub.10.1007/s004410051215", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1049837530", 
          "https://doi.org/10.1007/s004410051215"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1023/a:1025816809372", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1021644784", 
          "https://doi.org/10.1023/a:1025816809372"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/bf02890401", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1017343523", 
          "https://doi.org/10.1007/bf02890401"
        ], 
        "type": "CreativeWork"
      }
    ], 
    "datePublished": "2004-06", 
    "datePublishedReg": "2004-06-01", 
    "description": "Increased matrix metalloproteinase-12 (MMP-12) has been implicated in atherosclerosis and many other inflammatory processes. To define MMP-12 functions in vivo, we generated transgenic rabbits that expressed human (h) MMP-12 gene under the control of a macrophage-specific promoter, the human scavenger receptor promoter. Two transgenic founder rabbits were found to have hMMP-12 transgene integration by Southern blot analysis. hMMP-12 mRNA was expressed in peritoneal and alveolar macrophages, and in tissues enriched in macrophages in transgenic rabbits. High levels of hMMP-12 protein were detected in the conditioned media of cultured peritoneal and alveolar macrophages from transgenic rabbits. Zymography showed that hMMP-12 secreted from macrophages possessed enzymatic activity toward \u03b2-casein. To evaluate the expression of hMMP-12 in inflammatory sites, we used carrageenan-induced granulomas as an in vivo model for tissue macrophages and foam cells. Granuloma size in transgenic rabbits was significantly increased compared to that in control rabbits, and histological examination revealed that granulomas of transgenic rabbits were enriched in macrophages associated with increased hMMP-12 expression. We believe that this transgenic rabbit model with increased expression of hMMP-12 may become a useful model for further mechanistic studies of MMP-12 in inflammatory diseases and cancer invasion; it is also an ideal model for testing the in vivo action of MMP-12 inhibitors.", 
    "genre": "article", 
    "id": "sg:pub.10.1023/b:trag.0000034717.70729.61", 
    "inLanguage": "en", 
    "isAccessibleForFree": false, 
    "isPartOf": [
      {
        "id": "sg:journal.1102526", 
        "issn": [
          "0962-8819", 
          "1573-9368"
        ], 
        "name": "Transgenic Research", 
        "publisher": "Springer Nature", 
        "type": "Periodical"
      }, 
      {
        "issueNumber": "3", 
        "type": "PublicationIssue"
      }, 
      {
        "type": "PublicationVolume", 
        "volumeNumber": "13"
      }
    ], 
    "keywords": [
      "transgenic rabbits", 
      "transgenic founder rabbits", 
      "macrophage-specific promoter", 
      "Southern blot analysis", 
      "transgene integration", 
      "receptor promoter", 
      "Further mechanistic studies", 
      "enzymatic activity", 
      "blot analysis", 
      "founder rabbits", 
      "cancer invasion", 
      "promoter", 
      "transgenic rabbit model", 
      "matrix metalloproteinase-12", 
      "foam cells", 
      "macrophage-specific overexpression", 
      "expression", 
      "tissue macrophages", 
      "ideal model", 
      "alveolar macrophages", 
      "mechanistic studies", 
      "useful model", 
      "macrophages", 
      "genes", 
      "vivo model", 
      "protein", 
      "overexpression", 
      "vivo action", 
      "mRNA", 
      "high levels", 
      "metalloproteinase-12", 
      "invasion", 
      "cells", 
      "vivo", 
      "inhibitors", 
      "inflammatory diseases", 
      "inflammatory sites", 
      "zymography", 
      "casein", 
      "sites", 
      "tissue", 
      "activity", 
      "function", 
      "rabbits", 
      "medium", 
      "levels", 
      "action", 
      "inflammatory process", 
      "analysis", 
      "disease", 
      "process", 
      "size", 
      "atherosclerosis", 
      "control", 
      "study", 
      "model", 
      "integration", 
      "rabbit model", 
      "histological examination", 
      "granuloma size", 
      "carrageenan", 
      "examination", 
      "granulomas", 
      "control rabbits", 
      "human (h) MMP-12 gene", 
      "MMP-12 gene", 
      "human scavenger receptor promoter", 
      "scavenger receptor promoter", 
      "hMMP-12 transgene integration", 
      "hMMP-12 mRNA", 
      "hMMP-12 protein", 
      "expression of hMMP-12", 
      "hMMP-12", 
      "hMMP-12 expression", 
      "Human Matrix Metalloproteinase-12"
    ], 
    "name": "Macrophage-Specific Overexpression of Human Matrix Metalloproteinase-12 in Transgenic Rabbits", 
    "pagination": "261-269", 
    "productId": [
      {
        "name": "dimensions_id", 
        "type": "PropertyValue", 
        "value": [
          "pub.1003968565"
        ]
      }, 
      {
        "name": "doi", 
        "type": "PropertyValue", 
        "value": [
          "10.1023/b:trag.0000034717.70729.61"
        ]
      }, 
      {
        "name": "pubmed_id", 
        "type": "PropertyValue", 
        "value": [
          "15359603"
        ]
      }
    ], 
    "sameAs": [
      "https://doi.org/10.1023/b:trag.0000034717.70729.61", 
      "https://app.dimensions.ai/details/publication/pub.1003968565"
    ], 
    "sdDataset": "articles", 
    "sdDatePublished": "2022-01-01T18:14", 
    "sdLicense": "https://scigraph.springernature.com/explorer/license/", 
    "sdPublisher": {
      "name": "Springer Nature - SN SciGraph project", 
      "type": "Organization"
    }, 
    "sdSource": "s3://com-springernature-scigraph/baseset/20220101/entities/gbq_results/article/article_389.jsonl", 
    "type": "ScholarlyArticle", 
    "url": "https://doi.org/10.1023/b:trag.0000034717.70729.61"
  }
]
 

Download the RDF metadata as:  json-ld nt turtle xml License info

HOW TO GET THIS DATA PROGRAMMATICALLY:

JSON-LD is a popular format for linked data which is fully compatible with JSON.

curl -H 'Accept: application/ld+json' 'https://scigraph.springernature.com/pub.10.1023/b:trag.0000034717.70729.61'

N-Triples is a line-based linked data format ideal for batch operations.

curl -H 'Accept: application/n-triples' 'https://scigraph.springernature.com/pub.10.1023/b:trag.0000034717.70729.61'

Turtle is a human-readable linked data format.

curl -H 'Accept: text/turtle' 'https://scigraph.springernature.com/pub.10.1023/b:trag.0000034717.70729.61'

RDF/XML is a standard XML format for linked data.

curl -H 'Accept: application/rdf+xml' 'https://scigraph.springernature.com/pub.10.1023/b:trag.0000034717.70729.61'


 

This table displays all metadata directly associated to this object as RDF triples.

303 TRIPLES      22 PREDICATES      123 URIs      112 LITERALS      25 BLANK NODES

Subject Predicate Object
1 sg:pub.10.1023/b:trag.0000034717.70729.61 schema:about N32da8340798944ed903b821df4493125
2 N391702620ef24f3cbb92fefda56963c8
3 N3e13aec9501447a99dc1511e665f70ca
4 N4ce6ef2304994b9c8056c211b55a19a9
5 N4dcb112418664f9d9f97ede4266a2b44
6 N4e503925f6314ef08084cc2a0338cb6c
7 N526a460425e449eeba23508c6934ebd5
8 N7189cab23d9d41f2b2ea32b6bb908571
9 N7aa59f18ecb641208a6f236fc0dacaa6
10 N7f1240d962474b8f8e08f286d3d979eb
11 N82990c8aade64af68f352196542a69a9
12 N87189f069d4449d4924b0196482f83d5
13 N95411025770742d987b77619fda1fa21
14 Nacbb091678604a26b6dfb595c700e01c
15 Nc3f903e53ae24cdbaee4314aa6283a05
16 Nc725ac631e4148ae9bd561a44e2022ba
17 Ne82f67063c3949779da7199efe70849e
18 Nee40391619be428996d988c735d1e590
19 anzsrc-for:06
20 anzsrc-for:0601
21 schema:author Nc711368cfd6c410ea0ccd980519ff323
22 schema:citation sg:pub.10.1007/bf02890401
23 sg:pub.10.1007/s004410051215
24 sg:pub.10.1023/a:1025816809372
25 schema:datePublished 2004-06
26 schema:datePublishedReg 2004-06-01
27 schema:description Increased matrix metalloproteinase-12 (MMP-12) has been implicated in atherosclerosis and many other inflammatory processes. To define MMP-12 functions in vivo, we generated transgenic rabbits that expressed human (h) MMP-12 gene under the control of a macrophage-specific promoter, the human scavenger receptor promoter. Two transgenic founder rabbits were found to have hMMP-12 transgene integration by Southern blot analysis. hMMP-12 mRNA was expressed in peritoneal and alveolar macrophages, and in tissues enriched in macrophages in transgenic rabbits. High levels of hMMP-12 protein were detected in the conditioned media of cultured peritoneal and alveolar macrophages from transgenic rabbits. Zymography showed that hMMP-12 secreted from macrophages possessed enzymatic activity toward β-casein. To evaluate the expression of hMMP-12 in inflammatory sites, we used carrageenan-induced granulomas as an in vivo model for tissue macrophages and foam cells. Granuloma size in transgenic rabbits was significantly increased compared to that in control rabbits, and histological examination revealed that granulomas of transgenic rabbits were enriched in macrophages associated with increased hMMP-12 expression. We believe that this transgenic rabbit model with increased expression of hMMP-12 may become a useful model for further mechanistic studies of MMP-12 in inflammatory diseases and cancer invasion; it is also an ideal model for testing the in vivo action of MMP-12 inhibitors.
28 schema:genre article
29 schema:inLanguage en
30 schema:isAccessibleForFree false
31 schema:isPartOf N796501c17c6d48b8976858020eaae8eb
32 Nd0fb9712e885418287f4bfc44c36e1a0
33 sg:journal.1102526
34 schema:keywords Further mechanistic studies
35 Human Matrix Metalloproteinase-12
36 MMP-12 gene
37 Southern blot analysis
38 action
39 activity
40 alveolar macrophages
41 analysis
42 atherosclerosis
43 blot analysis
44 cancer invasion
45 carrageenan
46 casein
47 cells
48 control
49 control rabbits
50 disease
51 enzymatic activity
52 examination
53 expression
54 expression of hMMP-12
55 foam cells
56 founder rabbits
57 function
58 genes
59 granuloma size
60 granulomas
61 hMMP-12
62 hMMP-12 expression
63 hMMP-12 mRNA
64 hMMP-12 protein
65 hMMP-12 transgene integration
66 high levels
67 histological examination
68 human (h) MMP-12 gene
69 human scavenger receptor promoter
70 ideal model
71 inflammatory diseases
72 inflammatory process
73 inflammatory sites
74 inhibitors
75 integration
76 invasion
77 levels
78 mRNA
79 macrophage-specific overexpression
80 macrophage-specific promoter
81 macrophages
82 matrix metalloproteinase-12
83 mechanistic studies
84 medium
85 metalloproteinase-12
86 model
87 overexpression
88 process
89 promoter
90 protein
91 rabbit model
92 rabbits
93 receptor promoter
94 scavenger receptor promoter
95 sites
96 size
97 study
98 tissue
99 tissue macrophages
100 transgene integration
101 transgenic founder rabbits
102 transgenic rabbit model
103 transgenic rabbits
104 useful model
105 vivo
106 vivo action
107 vivo model
108 zymography
109 schema:name Macrophage-Specific Overexpression of Human Matrix Metalloproteinase-12 in Transgenic Rabbits
110 schema:pagination 261-269
111 schema:productId N8abed1411d0b4cc8a9ab0c16148062e5
112 N91ab6d1307514167b725d5633815b791
113 Nab4c976097ba4234a6cd9a10cf77cb80
114 schema:sameAs https://app.dimensions.ai/details/publication/pub.1003968565
115 https://doi.org/10.1023/b:trag.0000034717.70729.61
116 schema:sdDatePublished 2022-01-01T18:14
117 schema:sdLicense https://scigraph.springernature.com/explorer/license/
118 schema:sdPublisher N09004d5f89ed4770b15cccda9b546ac4
119 schema:url https://doi.org/10.1023/b:trag.0000034717.70729.61
120 sgo:license sg:explorer/license/
121 sgo:sdDataset articles
122 rdf:type schema:ScholarlyArticle
123 N05c126953eeb424b8fc3d361da2bdbad rdf:first sg:person.01172340432.80
124 rdf:rest N582c625bb0fe4bff809e6c4973d7c1a8
125 N09004d5f89ed4770b15cccda9b546ac4 schema:name Springer Nature - SN SciGraph project
126 rdf:type schema:Organization
127 N0bbd73ee484c4f9ca21ae17c925a71ee rdf:first sg:person.0762316763.77
128 rdf:rest Nfe095c6ee62e4aaf8618081d4a159f6c
129 N32da8340798944ed903b821df4493125 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
130 schema:name Macrophages
131 rdf:type schema:DefinedTerm
132 N391702620ef24f3cbb92fefda56963c8 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
133 schema:name Transformation, Genetic
134 rdf:type schema:DefinedTerm
135 N3919eca541914755aa0afbaca3a71a49 rdf:first sg:person.0625674757.78
136 rdf:rest N0bbd73ee484c4f9ca21ae17c925a71ee
137 N3e13aec9501447a99dc1511e665f70ca schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
138 schema:name DNA Primers
139 rdf:type schema:DefinedTerm
140 N4ce6ef2304994b9c8056c211b55a19a9 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
141 schema:name Animals, Genetically Modified
142 rdf:type schema:DefinedTerm
143 N4dcb112418664f9d9f97ede4266a2b44 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
144 schema:name Blotting, Southern
145 rdf:type schema:DefinedTerm
146 N4e503925f6314ef08084cc2a0338cb6c schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
147 schema:name Transgenes
148 rdf:type schema:DefinedTerm
149 N526a460425e449eeba23508c6934ebd5 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
150 schema:name Promoter Regions, Genetic
151 rdf:type schema:DefinedTerm
152 N582c625bb0fe4bff809e6c4973d7c1a8 rdf:first sg:person.011340333222.04
153 rdf:rest N3919eca541914755aa0afbaca3a71a49
154 N7189cab23d9d41f2b2ea32b6bb908571 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
155 schema:name Matrix Metalloproteinase 12
156 rdf:type schema:DefinedTerm
157 N796501c17c6d48b8976858020eaae8eb schema:volumeNumber 13
158 rdf:type schema:PublicationVolume
159 N7aa59f18ecb641208a6f236fc0dacaa6 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
160 schema:name Models, Animal
161 rdf:type schema:DefinedTerm
162 N7f1240d962474b8f8e08f286d3d979eb schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
163 schema:name Caseins
164 rdf:type schema:DefinedTerm
165 N8056e05a100b4400a6f117ad66dfe413 rdf:first Nfeca6d98f8674f1e940c6b42c3288ed7
166 rdf:rest N05c126953eeb424b8fc3d361da2bdbad
167 N805ae5ad61b6462b8de4dc6954af5bf1 rdf:first sg:person.01304023165.50
168 rdf:rest Nd503346c42584e18a786a08ed81bf070
169 N82990c8aade64af68f352196542a69a9 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
170 schema:name Animals
171 rdf:type schema:DefinedTerm
172 N87189f069d4449d4924b0196482f83d5 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
173 schema:name Rabbits
174 rdf:type schema:DefinedTerm
175 N8a8e90a6246442538fef2a7dc686eb1a rdf:first sg:person.01300565244.96
176 rdf:rest N9438dde3d1e14ac1aea2e0e686a292b1
177 N8abed1411d0b4cc8a9ab0c16148062e5 schema:name dimensions_id
178 schema:value pub.1003968565
179 rdf:type schema:PropertyValue
180 N91ab6d1307514167b725d5633815b791 schema:name doi
181 schema:value 10.1023/b:trag.0000034717.70729.61
182 rdf:type schema:PropertyValue
183 N9438dde3d1e14ac1aea2e0e686a292b1 rdf:first sg:person.01321234402.12
184 rdf:rest N8056e05a100b4400a6f117ad66dfe413
185 N95411025770742d987b77619fda1fa21 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
186 schema:name Receptors, Scavenger
187 rdf:type schema:DefinedTerm
188 Nab4c976097ba4234a6cd9a10cf77cb80 schema:name pubmed_id
189 schema:value 15359603
190 rdf:type schema:PropertyValue
191 Nacbb091678604a26b6dfb595c700e01c schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
192 schema:name Gene Expression
193 rdf:type schema:DefinedTerm
194 Nc3f903e53ae24cdbaee4314aa6283a05 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
195 schema:name Metalloendopeptidases
196 rdf:type schema:DefinedTerm
197 Nc711368cfd6c410ea0ccd980519ff323 rdf:first sg:person.01136672602.49
198 rdf:rest N8a8e90a6246442538fef2a7dc686eb1a
199 Nc725ac631e4148ae9bd561a44e2022ba schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
200 schema:name Granuloma
201 rdf:type schema:DefinedTerm
202 Nd0fb9712e885418287f4bfc44c36e1a0 schema:issueNumber 3
203 rdf:type schema:PublicationIssue
204 Nd503346c42584e18a786a08ed81bf070 rdf:first sg:person.012664624074.29
205 rdf:rest rdf:nil
206 Ne82f67063c3949779da7199efe70849e schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
207 schema:name Receptors, Immunologic
208 rdf:type schema:DefinedTerm
209 Nee40391619be428996d988c735d1e590 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
210 schema:name Humans
211 rdf:type schema:DefinedTerm
212 Nfe095c6ee62e4aaf8618081d4a159f6c rdf:first sg:person.01054341145.34
213 rdf:rest N805ae5ad61b6462b8de4dc6954af5bf1
214 Nfeca6d98f8674f1e940c6b42c3288ed7 schema:affiliation grid-institutes:None
215 schema:familyName Matsumoto
216 schema:givenName Shin-Ich
217 rdf:type schema:Person
218 anzsrc-for:06 schema:inDefinedTermSet anzsrc-for:
219 schema:name Biological Sciences
220 rdf:type schema:DefinedTerm
221 anzsrc-for:0601 schema:inDefinedTermSet anzsrc-for:
222 schema:name Biochemistry and Cell Biology
223 rdf:type schema:DefinedTerm
224 sg:journal.1102526 schema:issn 0962-8819
225 1573-9368
226 schema:name Transgenic Research
227 schema:publisher Springer Nature
228 rdf:type schema:Periodical
229 sg:person.01054341145.34 schema:affiliation grid-institutes:None
230 schema:familyName Shikama
231 schema:givenName Hisataka
232 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01054341145.34
233 rdf:type schema:Person
234 sg:person.011340333222.04 schema:affiliation grid-institutes:grid.20515.33
235 schema:familyName Koike
236 schema:givenName Tomonari
237 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.011340333222.04
238 rdf:type schema:Person
239 sg:person.01136672602.49 schema:affiliation grid-institutes:grid.20515.33
240 schema:familyName Fan
241 schema:givenName Jianglin
242 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01136672602.49
243 rdf:type schema:Person
244 sg:person.01172340432.80 schema:affiliation grid-institutes:grid.20515.33
245 schema:familyName Liang
246 schema:givenName Jingyan
247 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01172340432.80
248 rdf:type schema:Person
249 sg:person.012664624074.29 schema:affiliation grid-institutes:grid.412339.e
250 schema:familyName Watanabe
251 schema:givenName Teruo
252 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.012664624074.29
253 rdf:type schema:Person
254 sg:person.01300565244.96 schema:affiliation grid-institutes:grid.20515.33
255 schema:familyName Wang
256 schema:givenName Xiaofei
257 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01300565244.96
258 rdf:type schema:Person
259 sg:person.01304023165.50 schema:affiliation grid-institutes:grid.271052.3
260 schema:familyName Sasaguri
261 schema:givenName Yasuyuki
262 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01304023165.50
263 rdf:type schema:Person
264 sg:person.01321234402.12 schema:affiliation grid-institutes:grid.20515.33
265 schema:familyName Wu
266 schema:givenName Lihua
267 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01321234402.12
268 rdf:type schema:Person
269 sg:person.0625674757.78 schema:affiliation grid-institutes:grid.20515.33
270 schema:familyName Ichikawa
271 schema:givenName Tomonaga
272 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0625674757.78
273 rdf:type schema:Person
274 sg:person.0762316763.77 schema:affiliation grid-institutes:grid.411971.b
275 schema:familyName Sun
276 schema:givenName Huijun
277 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0762316763.77
278 rdf:type schema:Person
279 sg:pub.10.1007/bf02890401 schema:sameAs https://app.dimensions.ai/details/publication/pub.1017343523
280 https://doi.org/10.1007/bf02890401
281 rdf:type schema:CreativeWork
282 sg:pub.10.1007/s004410051215 schema:sameAs https://app.dimensions.ai/details/publication/pub.1049837530
283 https://doi.org/10.1007/s004410051215
284 rdf:type schema:CreativeWork
285 sg:pub.10.1023/a:1025816809372 schema:sameAs https://app.dimensions.ai/details/publication/pub.1021644784
286 https://doi.org/10.1023/a:1025816809372
287 rdf:type schema:CreativeWork
288 grid-institutes:None schema:alternateName Yamanouchi Pharmaceutical Company Research Institute, 305-8585, Tsukuba, Japan
289 schema:name Yamanouchi Pharmaceutical Company Research Institute, 305-8585, Tsukuba, Japan
290 rdf:type schema:Organization
291 grid-institutes:grid.20515.33 schema:alternateName Laboratory of Cardiovascular Disease, Department of Pathology, Institute of Basic Medical Sciences, University of Tsukuba, 305-8575, Tsukuba, Japan
292 schema:name Laboratory of Cardiovascular Disease, Department of Pathology, Institute of Basic Medical Sciences, University of Tsukuba, 305-8575, Tsukuba, Japan
293 rdf:type schema:Organization
294 grid-institutes:grid.271052.3 schema:alternateName Department of Pathology and Cell Biology, School of Medicine, University of Occupational and Environmental Health, 807-8555, Kitakyushu, Japan
295 schema:name Department of Pathology and Cell Biology, School of Medicine, University of Occupational and Environmental Health, 807-8555, Kitakyushu, Japan
296 rdf:type schema:Organization
297 grid-institutes:grid.411971.b schema:alternateName Department of Pharmacology, Dalian Medical University, 116027, Dalian, China
298 schema:name Department of Pharmacology, Dalian Medical University, 116027, Dalian, China
299 Laboratory of Cardiovascular Disease, Department of Pathology, Institute of Basic Medical Sciences, University of Tsukuba, 305-8575, Tsukuba, Japan
300 rdf:type schema:Organization
301 grid-institutes:grid.412339.e schema:alternateName Saga University, 840-8502, Saga, Japan
302 schema:name Saga University, 840-8502, Saga, Japan
303 rdf:type schema:Organization
 




Preview window. Press ESC to close (or click here)


...