Transformed Roots of Lupinus mutabilis: Induction, Culture and Isoflavone Biosynthesis View Full Text


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Article Info

DATE

2004-07

AUTHORS

Mehmet Babaoglu, Michael R. Davey, J. Brian Power, Frank Sporer, Michael Wink

ABSTRACT

Transformed roots of Lupinus mutabilis cv. Potosi induced by Agrobacterium rhizogenes strain R1601 were cultured on Murashige and Skoog-based medium lacking kanamycin sulphate, or with this antibiotic at 40 mg l−1. The neomycin phosphotransferase gene in the genome of transformed roots was confirmed by non-radioactive Southern hybridisation. Neomycin phosphotransferase protein was detected by ELISA. Transformed roots synthesised isoflavones, but not quinolizidine alkaloids; the latter are typical secondary metabolites of lupin normally produced in aerial parts of the plant. Genistein and 2′-hydroxygenistein, were the main secondary metabolites in cultured, transformed roots, whereas the glycoside genistin was more abundant in roots of non-transformed plants. Wighteone concentrations in transgenic roots were higher than those of non-transformed roots. Transformed roots produced twice the concentration of isoflavones compared with roots from non-transformed plants, indicating that Ri plasmid T-DNA genes modified isoflavone concentration and pattern of biosynthesis. More... »

PAGES

29-36

References to SciGraph publications

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    http://scigraph.springernature.com/pub.10.1023/b:ticu.0000020386.03780.ea

    DOI

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    DIMENSIONS

    https://app.dimensions.ai/details/publication/pub.1005943973


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