Identification and Mapping of chi115 Gene and DNA Markers Linked to It in Pea (Pisum sativum L.) View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

2004-07

AUTHORS

K. Cheghamirza, O. V. Koveza, F. A. Konovalov, S. A. Gostimsky

ABSTRACT

Chlorophyll mutant Chi115 was induced by ethylmethane sulfonate (EMS) treatment of seeds of genotype Torsdag in Moscow State University and is characterized by lighter plant color. The monogenic nature of the mutant was determined by analyzing the F2 population from a cross between two P. sativum genotypes, WL1238 and Chi115. To establish a local map around the chi115gene, the RAPD and ISSR techniques were used with 45 RAPD and 10 ISSR primers in combination with bulked segregant analysis (BSA). Linkage of 12 RAPDs and 2 ISSRs to the chi115locus was observed in analysis of F2 single plants. Two RAPD markers that were closely associated with the chi115 gene were converted into the sequence characterized amplified region (SCAR) markers. By lowering the LOD score to 2, the linkage group containing the chi115 gene could be linked to the b gene (color of the flower) on linkage group III. Nevertheless, to prove the result obtained, three CAPS markers Sodmt, TubA1, and Rb were chosen on linkage group III. The results of linkage analysis showed that these CAPS markers were located within the linkage group including thechi115gene. More... »

PAGES

737-742

Journal

TITLE

Russian Journal of Genetics

ISSUE

7

VOLUME

40

Author Affiliations

Identifiers

URI

http://scigraph.springernature.com/pub.10.1023/b:ruge.0000036522.14176.a3

DOI

http://dx.doi.org/10.1023/b:ruge.0000036522.14176.a3

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1014866221


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