Ontology type: schema:ScholarlyArticle
2004-01
AUTHORSDanny W-K Ng, Mahesh B. Chandrasekharan, Timothy C. Hall
ABSTRACTThe involvement of transcription factors Arabidopsis abscisic acid-insensitive3 (ABI3), maize viviparous1 (VP1) and Phaseolus vulgaris ABI3-like factor (PvALF) in the spatial control of storage protein gene expression is well established. However, little insight exists as to how they are themselves regulated. To address this, a 5.15 kb ABI3 upstream sequence including a 4.6 kb full-length promoter and 519 bp of 5′-untranslated region (UTR) was used to drive either β-glucuronidase (GUS) or green fluorescent protein (GFP) expression in Arabidopsis. Expression from the full-length (−4630/+519ABI3) and various 5′-truncated promoters was detected during embryogenesis in all lines, except those transgenic for promoter elements shorter than 364 bp. Two upstream activating regions, −3600 to −2033 and −2033 to −882, enhanced GUS expression in seeds. The −882 to −364 region was sufficient to confer seed-specific expression of GUS when fused to a −64/+6CaMV 35S minimal promoter. Expression from the ABI3 promoter constructs was seed-specific, except in the presence of exogenous abscisic acid (ABA) (>0.3 μM), when GUS expression was detected in seedling roots. Excision of a 405 bp region containing three upstream open reading frames (uORFs) from the 5′-UTR dramatically increased GUS expression and debilitated constraint of reporter expression in roots. Negative regulation of ABI3 expression by the 5′-UTR may involve a post-transcriptional mechanism analogous to that of tumor suppressor genes which also bear long, uORF-containing, 5′-UTRs, or through interactions with RNA-binding proteins. More... »
PAGES25-38
http://scigraph.springernature.com/pub.10.1023/b:plan.0000028767.06820.34
DOIhttp://dx.doi.org/10.1023/b:plan.0000028767.06820.34
DIMENSIONShttps://app.dimensions.ai/details/publication/pub.1036462652
PUBMEDhttps://www.ncbi.nlm.nih.gov/pubmed/15159632
JSON-LD is the canonical representation for SciGraph data.
TIP: You can open this SciGraph record using an external JSON-LD service: JSON-LD Playground Google SDTT
[
{
"@context": "https://springernature.github.io/scigraph/jsonld/sgcontext.json",
"about": [
{
"id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/06",
"inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/",
"name": "Biological Sciences",
"type": "DefinedTerm"
},
{
"id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/0604",
"inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/",
"name": "Genetics",
"type": "DefinedTerm"
},
{
"inDefinedTermSet": "https://www.nlm.nih.gov/mesh/",
"name": "5' Flanking Region",
"type": "DefinedTerm"
},
{
"inDefinedTermSet": "https://www.nlm.nih.gov/mesh/",
"name": "Abscisic Acid",
"type": "DefinedTerm"
},
{
"inDefinedTermSet": "https://www.nlm.nih.gov/mesh/",
"name": "Amino Acid Sequence",
"type": "DefinedTerm"
},
{
"inDefinedTermSet": "https://www.nlm.nih.gov/mesh/",
"name": "Arabidopsis",
"type": "DefinedTerm"
},
{
"inDefinedTermSet": "https://www.nlm.nih.gov/mesh/",
"name": "Arabidopsis Proteins",
"type": "DefinedTerm"
},
{
"inDefinedTermSet": "https://www.nlm.nih.gov/mesh/",
"name": "Base Sequence",
"type": "DefinedTerm"
},
{
"inDefinedTermSet": "https://www.nlm.nih.gov/mesh/",
"name": "Darkness",
"type": "DefinedTerm"
},
{
"inDefinedTermSet": "https://www.nlm.nih.gov/mesh/",
"name": "Gene Expression Regulation, Developmental",
"type": "DefinedTerm"
},
{
"inDefinedTermSet": "https://www.nlm.nih.gov/mesh/",
"name": "Gene Expression Regulation, Plant",
"type": "DefinedTerm"
},
{
"inDefinedTermSet": "https://www.nlm.nih.gov/mesh/",
"name": "Glucuronidase",
"type": "DefinedTerm"
},
{
"inDefinedTermSet": "https://www.nlm.nih.gov/mesh/",
"name": "Green Fluorescent Proteins",
"type": "DefinedTerm"
},
{
"inDefinedTermSet": "https://www.nlm.nih.gov/mesh/",
"name": "Light",
"type": "DefinedTerm"
},
{
"inDefinedTermSet": "https://www.nlm.nih.gov/mesh/",
"name": "Luminescent Proteins",
"type": "DefinedTerm"
},
{
"inDefinedTermSet": "https://www.nlm.nih.gov/mesh/",
"name": "Molecular Sequence Data",
"type": "DefinedTerm"
},
{
"inDefinedTermSet": "https://www.nlm.nih.gov/mesh/",
"name": "Mutation",
"type": "DefinedTerm"
},
{
"inDefinedTermSet": "https://www.nlm.nih.gov/mesh/",
"name": "Plant Growth Regulators",
"type": "DefinedTerm"
},
{
"inDefinedTermSet": "https://www.nlm.nih.gov/mesh/",
"name": "Plant Leaves",
"type": "DefinedTerm"
},
{
"inDefinedTermSet": "https://www.nlm.nih.gov/mesh/",
"name": "Plant Roots",
"type": "DefinedTerm"
},
{
"inDefinedTermSet": "https://www.nlm.nih.gov/mesh/",
"name": "Plants, Genetically Modified",
"type": "DefinedTerm"
},
{
"inDefinedTermSet": "https://www.nlm.nih.gov/mesh/",
"name": "Promoter Regions, Genetic",
"type": "DefinedTerm"
},
{
"inDefinedTermSet": "https://www.nlm.nih.gov/mesh/",
"name": "Recombinant Fusion Proteins",
"type": "DefinedTerm"
},
{
"inDefinedTermSet": "https://www.nlm.nih.gov/mesh/",
"name": "Regulatory Sequences, Nucleic Acid",
"type": "DefinedTerm"
},
{
"inDefinedTermSet": "https://www.nlm.nih.gov/mesh/",
"name": "Seeds",
"type": "DefinedTerm"
},
{
"inDefinedTermSet": "https://www.nlm.nih.gov/mesh/",
"name": "Transcription Factors",
"type": "DefinedTerm"
}
],
"author": [
{
"affiliation": {
"alternateName": "Institute of Developmental and Molecular Biology and Department of Biology, Texas A&M University, 77843-3155, College Station, TX, USA",
"id": "http://www.grid.ac/institutes/grid.264756.4",
"name": [
"Institute of Developmental and Molecular Biology and Department of Biology, Texas A&M University, 77843-3155, College Station, TX, USA"
],
"type": "Organization"
},
"familyName": "Ng",
"givenName": "Danny W-K",
"id": "sg:person.01344416205.91",
"sameAs": [
"https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01344416205.91"
],
"type": "Person"
},
{
"affiliation": {
"alternateName": "Institute of Developmental and Molecular Biology and Department of Biology, Texas A&M University, 77843-3155, College Station, TX, USA",
"id": "http://www.grid.ac/institutes/grid.264756.4",
"name": [
"Institute of Developmental and Molecular Biology and Department of Biology, Texas A&M University, 77843-3155, College Station, TX, USA"
],
"type": "Organization"
},
"familyName": "Chandrasekharan",
"givenName": "Mahesh B.",
"id": "sg:person.01262613325.91",
"sameAs": [
"https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01262613325.91"
],
"type": "Person"
},
{
"affiliation": {
"alternateName": "Institute of Developmental and Molecular Biology and Department of Biology, Texas A&M University, 77843-3155, College Station, TX, USA",
"id": "http://www.grid.ac/institutes/grid.264756.4",
"name": [
"Institute of Developmental and Molecular Biology and Department of Biology, Texas A&M University, 77843-3155, College Station, TX, USA"
],
"type": "Organization"
},
"familyName": "Hall",
"givenName": "Timothy C.",
"id": "sg:person.01332715617.81",
"sameAs": [
"https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01332715617.81"
],
"type": "Person"
}
],
"citation": [
{
"id": "sg:pub.10.1038/nature00936",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1049497123",
"https://doi.org/10.1038/nature00936"
],
"type": "CreativeWork"
},
{
"id": "sg:pub.10.1023/a:1010693703421",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1034341197",
"https://doi.org/10.1023/a:1010693703421"
],
"type": "CreativeWork"
},
{
"id": "sg:pub.10.1023/a:1013340004348",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1003060343",
"https://doi.org/10.1023/a:1013340004348"
],
"type": "CreativeWork"
},
{
"id": "sg:pub.10.1007/bf00020199",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1024363386",
"https://doi.org/10.1007/bf00020199"
],
"type": "CreativeWork"
},
{
"id": "sg:pub.10.1023/a:1006201910593",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1043091421",
"https://doi.org/10.1023/a:1006201910593"
],
"type": "CreativeWork"
},
{
"id": "sg:pub.10.1038/sj.onc.1206783",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1049113327",
"https://doi.org/10.1038/sj.onc.1206783"
],
"type": "CreativeWork"
}
],
"datePublished": "2004-01",
"datePublishedReg": "2004-01-01",
"description": "The involvement of transcription factors Arabidopsis abscisic acid-insensitive3 (ABI3), maize viviparous1 (VP1) and Phaseolus vulgaris ABI3-like factor (PvALF) in the spatial control of storage protein gene expression is well established. However, little insight exists as to how they are themselves regulated. To address this, a 5.15 kb ABI3 upstream sequence including a 4.6 kb full-length promoter and 519 bp of 5\u2032-untranslated region (UTR) was used to drive either \u03b2-glucuronidase (GUS) or green fluorescent protein (GFP) expression in Arabidopsis. Expression from the full-length (\u22124630/+519ABI3) and various 5\u2032-truncated promoters was detected during embryogenesis in all lines, except those transgenic for promoter elements shorter than 364 bp. Two upstream activating regions, \u22123600 to \u22122033 and \u22122033 to \u2212882, enhanced GUS expression in seeds. The \u2212882 to \u2212364 region was sufficient to confer seed-specific expression of GUS when fused to a \u221264/+6CaMV 35S minimal promoter. Expression from the ABI3 promoter constructs was seed-specific, except in the presence of exogenous abscisic acid (ABA) (>0.3 \u03bcM), when GUS expression was detected in seedling roots. Excision of a 405 bp region containing three upstream open reading frames (uORFs) from the 5\u2032-UTR dramatically increased GUS expression and debilitated constraint of reporter expression in roots. Negative regulation of ABI3 expression by the 5\u2032-UTR may involve a post-transcriptional mechanism analogous to that of tumor suppressor genes which also bear long, uORF-containing, 5\u2032-UTRs, or through interactions with RNA-binding proteins.",
"genre": "article",
"id": "sg:pub.10.1023/b:plan.0000028767.06820.34",
"inLanguage": "en",
"isAccessibleForFree": false,
"isPartOf": [
{
"id": "sg:journal.1101246",
"issn": [
"0167-4412",
"1573-5028"
],
"name": "Plant Molecular Biology",
"publisher": "Springer Nature",
"type": "Periodical"
},
{
"issueNumber": "1",
"type": "PublicationIssue"
},
{
"type": "PublicationVolume",
"volumeNumber": "54"
}
],
"keywords": [
"upstream open reading frames",
"GUS expression",
"abscisic acid",
"storage protein gene expression",
"ABSCISIC ACID INSENSITIVE3",
"seed-specific expression",
"exogenous abscisic acid",
"post-transcriptional mechanisms",
"open reading frame",
"full-length promoter",
"protein gene expression",
"green fluorescent protein expression",
"fluorescent protein expression",
"tumor suppressor gene",
"ABI3 promoter",
"ABI3 expression",
"minimal promoter",
"promoter elements",
"reading frame",
"upstream sequences",
"reporter expression",
"negative regulation",
"gene expression",
"promoter constructs",
"bp region",
"suppressor gene",
"spatial expression",
"promoter",
"VIVIPAROUS1",
"protein expression",
"UTR",
"spatial control",
"expression",
"glucuronidase",
"BP",
"Arabidopsis",
"roots",
"embryogenesis",
"genes",
"RNA",
"protein",
"transgenic",
"seeds",
"regulation",
"region",
"little insight",
"sequence",
"acid",
"insights",
"mechanism",
"lines",
"interaction",
"constructs",
"frame",
"involvement",
"presence",
"factors",
"elements",
"control",
"constraints",
"excision"
],
"name": "The 5\u2032 UTR negatively regulates quantitative and spatial expression from the ABI3 promoter",
"pagination": "25-38",
"productId": [
{
"name": "dimensions_id",
"type": "PropertyValue",
"value": [
"pub.1036462652"
]
},
{
"name": "doi",
"type": "PropertyValue",
"value": [
"10.1023/b:plan.0000028767.06820.34"
]
},
{
"name": "pubmed_id",
"type": "PropertyValue",
"value": [
"15159632"
]
}
],
"sameAs": [
"https://doi.org/10.1023/b:plan.0000028767.06820.34",
"https://app.dimensions.ai/details/publication/pub.1036462652"
],
"sdDataset": "articles",
"sdDatePublished": "2022-06-01T22:03",
"sdLicense": "https://scigraph.springernature.com/explorer/license/",
"sdPublisher": {
"name": "Springer Nature - SN SciGraph project",
"type": "Organization"
},
"sdSource": "s3://com-springernature-scigraph/baseset/20220601/entities/gbq_results/article/article_389.jsonl",
"type": "ScholarlyArticle",
"url": "https://doi.org/10.1023/b:plan.0000028767.06820.34"
}
]Download the RDF metadata as: json-ld nt turtle xml License info
JSON-LD is a popular format for linked data which is fully compatible with JSON.
curl -H 'Accept: application/ld+json' 'https://scigraph.springernature.com/pub.10.1023/b:plan.0000028767.06820.34'
N-Triples is a line-based linked data format ideal for batch operations.
curl -H 'Accept: application/n-triples' 'https://scigraph.springernature.com/pub.10.1023/b:plan.0000028767.06820.34'
Turtle is a human-readable linked data format.
curl -H 'Accept: text/turtle' 'https://scigraph.springernature.com/pub.10.1023/b:plan.0000028767.06820.34'
RDF/XML is a standard XML format for linked data.
curl -H 'Accept: application/rdf+xml' 'https://scigraph.springernature.com/pub.10.1023/b:plan.0000028767.06820.34'
This table displays all metadata directly associated to this object as RDF triples.
257 TRIPLES
22 PREDICATES
118 URIs
104 LITERALS
31 BLANK NODES