Lactase in Immobilized Cells of Watermelon View Full Text


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Article Info

DATE

2003-07

AUTHORS

I. Tilemann, E. Tokhtaeva, E. Sedlarova, A. Barth, A. Valent, P. Siekel, M. Duricek

ABSTRACT

A cell suspension culture of Citrullus vulgaris Schrad cv. “Samara” was permeabilized by Tween 80 and immobilized by glutaraldehyde. The highest lactase activity was achieved at pH 4.3, the temperature optimum for cell suspension was at 50°C, while for the immobilized cells the optimum was at 58°C. The hydrolysis of substrate was linear for 3 h, reaching 60-67% conversion rate. The cells were characterized by high enzyme activity. The stability of the enzyme showed convenient physico-mechanical properties (physical protection from shear forces and easy separation of product from biocatalysts) in long-term storage. More... »

PAGES

394-398

References to SciGraph publications

  • 1987-09. Immobilization of yeast cells by adhesion on tuff granules in APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
  • 2002-06. Invertase in Immobilized Cells of Eschscholtzia Californica in BIOLOGIA PLANTARUM
  • 1990-11. Immobilization of lactase for the continuous hydrolysis of whey permaete in BIOPROCESS AND BIOSYSTEMS ENGINEERING
  • Identifiers

    URI

    http://scigraph.springernature.com/pub.10.1023/b:conc.0000003424.33324.e4

    DOI

    http://dx.doi.org/10.1023/b:conc.0000003424.33324.e4

    DIMENSIONS

    https://app.dimensions.ai/details/publication/pub.1030271201


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