Ontology type: schema:ScholarlyArticle
2004-06
AUTHORSXue-Ming Tang, Wei Shen, F.M. Lakay, Wei-Lan Shao, Zheng-Xiang Wang, B.A. Prior, Jian Zhuge
ABSTRACTThe alkaline protease gene, apr, from Bacillus licheniformis 2709 was cloned into a Bacillus shuttle expression vector, pHL, to yield the recombinant plasmid pHL-apr. The pHL-apr was expressed in Bacillus subtilis WB600, yielding a high expression strain BW-016. The amount of alkaline protease produced in the recombinant increased by 65% relative to the original strain. SDS-PAGE analysis indicated a Mr of 30.5 kDa. The amino acid sequence deduced from the DNA sequence analysis revealed a 98% identity to that of Bacillus licheniformis 6816. More... »
PAGES975-979
http://scigraph.springernature.com/pub.10.1023/b:bile.0000030042.91094.38
DOIhttp://dx.doi.org/10.1023/b:bile.0000030042.91094.38
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