Microbial monitoring by molecular tools of a two-phase anaerobic bioreactor treating fruit and vegetable wastes View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

2004-05

AUTHORS

H. Bouallagui, M. Torrijos, J.J. Godon, R. Moletta, R. Ben Cheikh, Y. Touhami, J.P. Delgenes, M. Hamdi

ABSTRACT

Microbial consortia in a two-phase, anaerobic bioreactor using a mixture of fruit and vegetable wastes were established. Bacterial and archaeal communities obtained by a culture-independent approach based on single strand conformation polymorphism analysis of total 16S rDNA showed the adaptation of the microflora to the process parameters. Throughout the 90 d of the study, the species composition of the bacterial community changed significantly. Bacterial 16S rDNA showed at least 7 different major species with a very prominent one corresponding to a Megasphaera elsdenii whereas bacterial 16S rDNA of a methanization bioreactor showed 10 different major species. After two weeks, Prevotella ruminicola became major and its dominance increased continuously until day 50. After an acid shock at pH 5, the 16S rDNA archaeal patterns in the acidogenic reactor showed two major prominent species corresponding to Methanosphaera stadtmanii and Methanobrevibacter wolinii, a hydrogenotrophic bacterium. More... »

PAGES

857-862

Identifiers

URI

http://scigraph.springernature.com/pub.10.1023/b:bile.0000025892.19733.18

DOI

http://dx.doi.org/10.1023/b:bile.0000025892.19733.18

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1006385284

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/15269562


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