Differential Properties Between an Endogenous Brain Na+, K+-ATPase Inhibitor and Ouabain View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

1997-04

AUTHORS

Clara Peña, Georgina Rodríguez de Lores Arnaiz

ABSTRACT

By means of a Sephadex G-50 column and anionic exchange HPLC a cerebral cortex soluble fraction (II-E) which highly inhibits neuronal Na(+)-K(+)-ATPase activity has been previously obtained. Herein, II-E properties are compared with those of the cardenolide ouabain, the selective and specific Na+, K(+)-ATPase inhibitor. It was observed that alkali treatment destroyed II-E but not ouabain inhibitory activity. II-E presented a maximal absorbance at 265 nm both at pH 7 and pH 2 which diminished at pH 10. Ouabain showed a maximum at 220 nm which was not altered by alkalinization. II-E was not retained in a C-18 column, indicating its hydrophilic nature, whereas ouabain presented a 26-min retention time in reverse phase HPLC. Therefore, it is concluded that the inhibitory factor present in II-E is structurally different to ouabain. More... »

PAGES

379-383

Identifiers

URI

http://scigraph.springernature.com/pub.10.1023/a:1027343323629

DOI

http://dx.doi.org/10.1023/a:1027343323629

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1049344882

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/9130247


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