Heterologous Kluyveromyces lactis β-galactosidase secretion by Saccharomyces cerevisiae super-secreting mutants View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

2001-01

AUTHORS

M. Becerra, S. Díaz Prado, E. Cerdán, M.I. González Siso

ABSTRACT

Several Saccharomyces cerevisiae strains with a super-secreting phenotype have been transformed using a secretion plasmid containing the LAC4 gene and have proven to be effective in the secretion of Kluyveromyceslactis β-galactosidase. The strain CGY1585 (ssc1-1) showed the highest secretion (1.7 EU ml−1) in the culture medium. As far as we know, Kluyveromyceslactis β-galactosidase is the largest sized protein and the only intracellular one among those secreted by these mutants hitherto. The recombinant strains all grew in lactose media. More... »

PAGES

33-40

Identifiers

URI

http://scigraph.springernature.com/pub.10.1023/a:1026795706520

DOI

http://dx.doi.org/10.1023/a:1026795706520

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1007928027


Indexing Status Check whether this publication has been indexed by Scopus and Web Of Science using the SN Indexing Status Tool
Incoming Citations Browse incoming citations for this publication using opencitations.net

JSON-LD is the canonical representation for SciGraph data.

TIP: You can open this SciGraph record using an external JSON-LD service: JSON-LD Playground Google SDTT

[
  {
    "@context": "https://springernature.github.io/scigraph/jsonld/sgcontext.json", 
    "about": [
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/0601", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Biochemistry and Cell Biology", 
        "type": "DefinedTerm"
      }, 
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/06", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Biological Sciences", 
        "type": "DefinedTerm"
      }
    ], 
    "author": [
      {
        "affiliation": {
          "name": [
            "Departamento de Biolox\u00eda Celular e Molecular, Facultade de Ciencias, Campus da Zapateira s/n, 15071-A Coru\u00f1a, Spain"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Becerra", 
        "givenName": "M.", 
        "id": "sg:person.01035705111.71", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01035705111.71"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "name": [
            "Departamento de Biolox\u00eda Celular e Molecular, Facultade de Ciencias, Campus da Zapateira s/n, 15071-A Coru\u00f1a, Spain"
          ], 
          "type": "Organization"
        }, 
        "familyName": "D\u00edaz Prado", 
        "givenName": "S.", 
        "id": "sg:person.015730575154.33", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.015730575154.33"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "name": [
            "Departamento de Biolox\u00eda Celular e Molecular, Facultade de Ciencias, Campus da Zapateira s/n, 15071-A Coru\u00f1a, Spain"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Cerd\u00e1n", 
        "givenName": "E.", 
        "id": "sg:person.0601204117.18", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0601204117.18"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "name": [
            "Departamento de Biolox\u00eda Celular e Molecular, Facultade de Ciencias, Campus da Zapateira s/n, 15071-A Coru\u00f1a, Spain"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Gonz\u00e1lez Siso", 
        "givenName": "M.I.", 
        "id": "sg:person.011245364025.32", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.011245364025.32"
        ], 
        "type": "Person"
      }
    ], 
    "citation": [
      {
        "id": "sg:pub.10.1007/bf01021064", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1003233988", 
          "https://doi.org/10.1007/bf01021064"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1002/(sici)1097-0061(19980630)14:9<827::aid-yea281>3.0.co;2-n", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1006224653"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1002/(sici)1097-0061(19980630)14:9<827::aid-yea281>3.0.co;2-n", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1006224653"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/0960-8524(96)00036-3", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1011169947"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/s0304-4165(97)00048-2", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1011544794"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1002/bit.260390802", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1016742280"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/0076-6879(83)01013-7", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1019090072"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/0378-1119(88)90185-0", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1031424271"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/0378-1119(88)90185-0", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1031424271"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1002/(sici)1097-0061(19990930)15:13<1299::aid-yea454>3.0.co;2-v", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1034332414"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/0378-1119(91)90329-a", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1035153039"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/0378-1119(91)90329-a", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1035153039"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/0378-1119(93)90318-w", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1043883873"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/0378-1119(93)90318-w", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1043883873"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1023/a:1008885827560", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1044428070", 
          "https://doi.org/10.1023/a:1008885827560"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/0092-8674(89)90410-8", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1050227122"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1111/j.1470-8744.1998.tb01378.x", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1061939103"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1126/science.3939723", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1062622574"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://app.dimensions.ai/details/publication/pub.1078294835", 
        "type": "CreativeWork"
      }
    ], 
    "datePublished": "2001-01", 
    "datePublishedReg": "2001-01-01", 
    "description": "Several Saccharomyces cerevisiae strains with a super-secreting phenotype have been transformed using a secretion plasmid containing the LAC4 gene and have proven to be effective in the secretion of Kluyveromyceslactis \u03b2-galactosidase. The strain CGY1585 (ssc1-1) showed the highest secretion (1.7 EU ml\u22121) in the culture medium. As far as we know, Kluyveromyceslactis \u03b2-galactosidase is the largest sized protein and the only intracellular one among those secreted by these mutants hitherto. The recombinant strains all grew in lactose media.", 
    "genre": "research_article", 
    "id": "sg:pub.10.1023/a:1026795706520", 
    "inLanguage": [
      "en"
    ], 
    "isAccessibleForFree": false, 
    "isPartOf": [
      {
        "id": "sg:journal.1289037", 
        "issn": [
          "0951-208X", 
          "1573-6784"
        ], 
        "name": "Biotechnology Letters", 
        "type": "Periodical"
      }, 
      {
        "issueNumber": "1", 
        "type": "PublicationIssue"
      }, 
      {
        "type": "PublicationVolume", 
        "volumeNumber": "23"
      }
    ], 
    "name": "Heterologous Kluyveromyces lactis \u03b2-galactosidase secretion by Saccharomyces cerevisiae super-secreting mutants", 
    "pagination": "33-40", 
    "productId": [
      {
        "name": "readcube_id", 
        "type": "PropertyValue", 
        "value": [
          "25c4588823a49e3ea44195f997384c2be5c8faeba8b0209594b8a16a0c5a64f5"
        ]
      }, 
      {
        "name": "doi", 
        "type": "PropertyValue", 
        "value": [
          "10.1023/a:1026795706520"
        ]
      }, 
      {
        "name": "dimensions_id", 
        "type": "PropertyValue", 
        "value": [
          "pub.1007928027"
        ]
      }
    ], 
    "sameAs": [
      "https://doi.org/10.1023/a:1026795706520", 
      "https://app.dimensions.ai/details/publication/pub.1007928027"
    ], 
    "sdDataset": "articles", 
    "sdDatePublished": "2019-04-10T14:58", 
    "sdLicense": "https://scigraph.springernature.com/explorer/license/", 
    "sdPublisher": {
      "name": "Springer Nature - SN SciGraph project", 
      "type": "Organization"
    }, 
    "sdSource": "s3://com-uberresearch-data-dimensions-target-20181106-alternative/cleanup/v134/2549eaecd7973599484d7c17b260dba0a4ecb94b/merge/v9/a6c9fde33151104705d4d7ff012ea9563521a3ce/jats-lookup/v90/0000000001_0000000264/records_8663_00000503.jsonl", 
    "type": "ScholarlyArticle", 
    "url": "http://link.springer.com/10.1023%2FA%3A1026795706520"
  }
]
 

Download the RDF metadata as:  json-ld nt turtle xml License info

HOW TO GET THIS DATA PROGRAMMATICALLY:

JSON-LD is a popular format for linked data which is fully compatible with JSON.

curl -H 'Accept: application/ld+json' 'https://scigraph.springernature.com/pub.10.1023/a:1026795706520'

N-Triples is a line-based linked data format ideal for batch operations.

curl -H 'Accept: application/n-triples' 'https://scigraph.springernature.com/pub.10.1023/a:1026795706520'

Turtle is a human-readable linked data format.

curl -H 'Accept: text/turtle' 'https://scigraph.springernature.com/pub.10.1023/a:1026795706520'

RDF/XML is a standard XML format for linked data.

curl -H 'Accept: application/rdf+xml' 'https://scigraph.springernature.com/pub.10.1023/a:1026795706520'


 

This table displays all metadata directly associated to this object as RDF triples.

133 TRIPLES      21 PREDICATES      42 URIs      19 LITERALS      7 BLANK NODES

Subject Predicate Object
1 sg:pub.10.1023/a:1026795706520 schema:about anzsrc-for:06
2 anzsrc-for:0601
3 schema:author N87a54dabcde544c4ad1b16143227fa40
4 schema:citation sg:pub.10.1007/bf01021064
5 sg:pub.10.1023/a:1008885827560
6 https://app.dimensions.ai/details/publication/pub.1078294835
7 https://doi.org/10.1002/(sici)1097-0061(19980630)14:9<827::aid-yea281>3.0.co;2-n
8 https://doi.org/10.1002/(sici)1097-0061(19990930)15:13<1299::aid-yea454>3.0.co;2-v
9 https://doi.org/10.1002/bit.260390802
10 https://doi.org/10.1016/0076-6879(83)01013-7
11 https://doi.org/10.1016/0092-8674(89)90410-8
12 https://doi.org/10.1016/0378-1119(88)90185-0
13 https://doi.org/10.1016/0378-1119(91)90329-a
14 https://doi.org/10.1016/0378-1119(93)90318-w
15 https://doi.org/10.1016/0960-8524(96)00036-3
16 https://doi.org/10.1016/s0304-4165(97)00048-2
17 https://doi.org/10.1111/j.1470-8744.1998.tb01378.x
18 https://doi.org/10.1126/science.3939723
19 schema:datePublished 2001-01
20 schema:datePublishedReg 2001-01-01
21 schema:description Several Saccharomyces cerevisiae strains with a super-secreting phenotype have been transformed using a secretion plasmid containing the LAC4 gene and have proven to be effective in the secretion of Kluyveromyceslactis β-galactosidase. The strain CGY1585 (ssc1-1) showed the highest secretion (1.7 EU ml−1) in the culture medium. As far as we know, Kluyveromyceslactis β-galactosidase is the largest sized protein and the only intracellular one among those secreted by these mutants hitherto. The recombinant strains all grew in lactose media.
22 schema:genre research_article
23 schema:inLanguage en
24 schema:isAccessibleForFree false
25 schema:isPartOf N6caa360d65b54fcf92ef543d38080e02
26 N7f69e62224994c4980f6b7b7f16292f6
27 sg:journal.1289037
28 schema:name Heterologous Kluyveromyces lactis β-galactosidase secretion by Saccharomyces cerevisiae super-secreting mutants
29 schema:pagination 33-40
30 schema:productId N2a41c6859a4c4810a05ae0d4d232cbcd
31 N499b56d3df0445958ff596c761d213ba
32 N71edb60d1cf04dd8bb9a9b968048d500
33 schema:sameAs https://app.dimensions.ai/details/publication/pub.1007928027
34 https://doi.org/10.1023/a:1026795706520
35 schema:sdDatePublished 2019-04-10T14:58
36 schema:sdLicense https://scigraph.springernature.com/explorer/license/
37 schema:sdPublisher Nb2abeacbea044cf9a12605c76db7ef69
38 schema:url http://link.springer.com/10.1023%2FA%3A1026795706520
39 sgo:license sg:explorer/license/
40 sgo:sdDataset articles
41 rdf:type schema:ScholarlyArticle
42 N2273fa4644854705b99bcf75f34cf9d8 rdf:first sg:person.0601204117.18
43 rdf:rest N87af144c5051436b8cc89b0bd33e9371
44 N2a41c6859a4c4810a05ae0d4d232cbcd schema:name doi
45 schema:value 10.1023/a:1026795706520
46 rdf:type schema:PropertyValue
47 N499b56d3df0445958ff596c761d213ba schema:name dimensions_id
48 schema:value pub.1007928027
49 rdf:type schema:PropertyValue
50 N6caa360d65b54fcf92ef543d38080e02 schema:issueNumber 1
51 rdf:type schema:PublicationIssue
52 N71edb60d1cf04dd8bb9a9b968048d500 schema:name readcube_id
53 schema:value 25c4588823a49e3ea44195f997384c2be5c8faeba8b0209594b8a16a0c5a64f5
54 rdf:type schema:PropertyValue
55 N7e0ddf1320554b529ebcf5284f5c6741 schema:name Departamento de Bioloxía Celular e Molecular, Facultade de Ciencias, Campus da Zapateira s/n, 15071-A Coruña, Spain
56 rdf:type schema:Organization
57 N7f69e62224994c4980f6b7b7f16292f6 schema:volumeNumber 23
58 rdf:type schema:PublicationVolume
59 N87a54dabcde544c4ad1b16143227fa40 rdf:first sg:person.01035705111.71
60 rdf:rest Nd8f03abc2f27444589239856e9bd445f
61 N87af144c5051436b8cc89b0bd33e9371 rdf:first sg:person.011245364025.32
62 rdf:rest rdf:nil
63 Na7d69cc4c8cf4f2f8006af09221f3a66 schema:name Departamento de Bioloxía Celular e Molecular, Facultade de Ciencias, Campus da Zapateira s/n, 15071-A Coruña, Spain
64 rdf:type schema:Organization
65 Nb2abeacbea044cf9a12605c76db7ef69 schema:name Springer Nature - SN SciGraph project
66 rdf:type schema:Organization
67 Nc417c7865e264fc28b68007b4f6602c7 schema:name Departamento de Bioloxía Celular e Molecular, Facultade de Ciencias, Campus da Zapateira s/n, 15071-A Coruña, Spain
68 rdf:type schema:Organization
69 Nc57b9929e59045b89ea1675f6916b30a schema:name Departamento de Bioloxía Celular e Molecular, Facultade de Ciencias, Campus da Zapateira s/n, 15071-A Coruña, Spain
70 rdf:type schema:Organization
71 Nd8f03abc2f27444589239856e9bd445f rdf:first sg:person.015730575154.33
72 rdf:rest N2273fa4644854705b99bcf75f34cf9d8
73 anzsrc-for:06 schema:inDefinedTermSet anzsrc-for:
74 schema:name Biological Sciences
75 rdf:type schema:DefinedTerm
76 anzsrc-for:0601 schema:inDefinedTermSet anzsrc-for:
77 schema:name Biochemistry and Cell Biology
78 rdf:type schema:DefinedTerm
79 sg:journal.1289037 schema:issn 0951-208X
80 1573-6784
81 schema:name Biotechnology Letters
82 rdf:type schema:Periodical
83 sg:person.01035705111.71 schema:affiliation Nc417c7865e264fc28b68007b4f6602c7
84 schema:familyName Becerra
85 schema:givenName M.
86 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01035705111.71
87 rdf:type schema:Person
88 sg:person.011245364025.32 schema:affiliation N7e0ddf1320554b529ebcf5284f5c6741
89 schema:familyName González Siso
90 schema:givenName M.I.
91 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.011245364025.32
92 rdf:type schema:Person
93 sg:person.015730575154.33 schema:affiliation Na7d69cc4c8cf4f2f8006af09221f3a66
94 schema:familyName Díaz Prado
95 schema:givenName S.
96 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.015730575154.33
97 rdf:type schema:Person
98 sg:person.0601204117.18 schema:affiliation Nc57b9929e59045b89ea1675f6916b30a
99 schema:familyName Cerdán
100 schema:givenName E.
101 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0601204117.18
102 rdf:type schema:Person
103 sg:pub.10.1007/bf01021064 schema:sameAs https://app.dimensions.ai/details/publication/pub.1003233988
104 https://doi.org/10.1007/bf01021064
105 rdf:type schema:CreativeWork
106 sg:pub.10.1023/a:1008885827560 schema:sameAs https://app.dimensions.ai/details/publication/pub.1044428070
107 https://doi.org/10.1023/a:1008885827560
108 rdf:type schema:CreativeWork
109 https://app.dimensions.ai/details/publication/pub.1078294835 schema:CreativeWork
110 https://doi.org/10.1002/(sici)1097-0061(19980630)14:9<827::aid-yea281>3.0.co;2-n schema:sameAs https://app.dimensions.ai/details/publication/pub.1006224653
111 rdf:type schema:CreativeWork
112 https://doi.org/10.1002/(sici)1097-0061(19990930)15:13<1299::aid-yea454>3.0.co;2-v schema:sameAs https://app.dimensions.ai/details/publication/pub.1034332414
113 rdf:type schema:CreativeWork
114 https://doi.org/10.1002/bit.260390802 schema:sameAs https://app.dimensions.ai/details/publication/pub.1016742280
115 rdf:type schema:CreativeWork
116 https://doi.org/10.1016/0076-6879(83)01013-7 schema:sameAs https://app.dimensions.ai/details/publication/pub.1019090072
117 rdf:type schema:CreativeWork
118 https://doi.org/10.1016/0092-8674(89)90410-8 schema:sameAs https://app.dimensions.ai/details/publication/pub.1050227122
119 rdf:type schema:CreativeWork
120 https://doi.org/10.1016/0378-1119(88)90185-0 schema:sameAs https://app.dimensions.ai/details/publication/pub.1031424271
121 rdf:type schema:CreativeWork
122 https://doi.org/10.1016/0378-1119(91)90329-a schema:sameAs https://app.dimensions.ai/details/publication/pub.1035153039
123 rdf:type schema:CreativeWork
124 https://doi.org/10.1016/0378-1119(93)90318-w schema:sameAs https://app.dimensions.ai/details/publication/pub.1043883873
125 rdf:type schema:CreativeWork
126 https://doi.org/10.1016/0960-8524(96)00036-3 schema:sameAs https://app.dimensions.ai/details/publication/pub.1011169947
127 rdf:type schema:CreativeWork
128 https://doi.org/10.1016/s0304-4165(97)00048-2 schema:sameAs https://app.dimensions.ai/details/publication/pub.1011544794
129 rdf:type schema:CreativeWork
130 https://doi.org/10.1111/j.1470-8744.1998.tb01378.x schema:sameAs https://app.dimensions.ai/details/publication/pub.1061939103
131 rdf:type schema:CreativeWork
132 https://doi.org/10.1126/science.3939723 schema:sameAs https://app.dimensions.ai/details/publication/pub.1062622574
133 rdf:type schema:CreativeWork
 




Preview window. Press ESC to close (or click here)


...