Ontology type: schema:ScholarlyArticle
1999-08
AUTHORSKouhei Fukushima, Iwao Sasaki, Shun Sato, Hironobu Sasano, Z. Krozowski, Seiki Matsuno
ABSTRACTMineralocorticoid action is essential forcolonic sodium and water absorption and is mediated viamineralocorticoid receptors in the upper half of coloniccrypts. On the other hand, it has been established that sodium butyrate inducesdifferentiation-like phenomenon in vitro. The aim ofthis study is to investigate whether this bacterialproduct participates in the regulation of gene andprotein expression of mineralocorticoid receptor in vitro. IEC6and T84 cells were stimulated by sodium butyrate andRNAs extracted. Gene expression of mineralocorticoidreceptor was evaluated by northern blotting orsemiquantitative RT-PCR. Protein expression was determined inT84 cells using immunohistochemistry. To investigatewhether MR induction was associated with cellulardifferentiation, we also measured alkaline phosphatase in situ. The mineralocorticoid receptor genewas induced by sodium butyrate in both IEC6 and T84cells. Immunoreactivity increased in butyrate-treatedT84 cells, but receptorcontaining cells were notuniformly distributed and often formed clusters.Induction of alkaline phosphate activity was alsodemonstrated in both IEC6 and T84 cells. Double stainingby immunoreactivity and alkaline phosphatase activityclearly demonstrated the colocalization of both afterbutyrate treatment. In conclusion, sodium butyrateup-regulates gene and protein expression of thefunctionally important mineralocorticoid receptor inepithelial cells, after induction by differentiation-likecondition in vitro. More... »
PAGES1571-1578
http://scigraph.springernature.com/pub.10.1023/a:1026606809171
DOIhttp://dx.doi.org/10.1023/a:1026606809171
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PUBMEDhttps://www.ncbi.nlm.nih.gov/pubmed/10492134
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