Transient decreases in methylation at 5′-CCGG-3′ sequences in potato (Solanum tuberosum L.) meristem DNA during progression of tubers through dormancy ... View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

2003-02

AUTHORS

R. David Law, Jeffrey C. Suttle

ABSTRACT

The 5-methylcytosine (5mC) content in DNA of tuber meristems isolated from field-grown potatoes (Solanum tuberosum L.) was determined during a 7-month storage period at 3 degrees C for three growing/postharvest seasons. No significant changes in 5mC levels were noted genome-wide or within 5'-CG-3' dinucleotide sequences, 5'-CG-3' islands or 5'-CA(T)G-3' trinucleotide sequences during storage. However, a consistent but transient 50-70% decrease in methylation at both cytosines within 5'-CCGG-3' sequences was detected that peaked 112-194 days after harvest. This result was corroborated by methylation-sensitive amplified fragment length polymorphism analysis of meristem DNA. Similar to tuber meristems undergoing progression through natural dormancy, premature chemical termination of dormancy resulted in rapid, transient 5'-CCGG-3' demethylation in meristem DNA. Minimum methylation levels at this sequence preceded initiation of high levels of de novo DNA synthesis by two days. Cytosine methylation status was also followed in in vitro-generated potato microtubers during 7 months of post-harvest storage. As in DNA from tuber bud meristems, no changes in genome-wide 5mC content or methylation at 5'-CA(T)G-3' or 5'-CG-3' island sequences were noted in microtuber DNA. However, there was a transient 46% drop in methylation at 5'-CG-3' dinucleotides concomitant with minimum levels of 5'-CCGG-3' methylation (30-60% below those in dormant microtubers) 57-98 days after harvest. As microtubers exited dormancy, there were sustained three- and seven-fold increases in RNA and DNA synthesis rates, peaking on or after 98 days of storage, respectively. Together, these data demonstrate that demethylation of 5'-CCGG-3' sequences occurs independently of tuber age during dormancy progression and precedes transcriptional activation of genes leading to cell division and meristem growth in potatoes. More... »

PAGES

437-447

Identifiers

URI

http://scigraph.springernature.com/pub.10.1023/a:1022002304479

DOI

http://dx.doi.org/10.1023/a:1022002304479

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1017755717

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/12602873


Indexing Status Check whether this publication has been indexed by Scopus and Web Of Science using the SN Indexing Status Tool
Incoming Citations Browse incoming citations for this publication using opencitations.net

JSON-LD is the canonical representation for SciGraph data.

TIP: You can open this SciGraph record using an external JSON-LD service: JSON-LD Playground Google SDTT

[
  {
    "@context": "https://springernature.github.io/scigraph/jsonld/sgcontext.json", 
    "about": [
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/0604", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Genetics", 
        "type": "DefinedTerm"
      }, 
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/06", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Biological Sciences", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "5-Methylcytosine", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Base Sequence", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Binding Sites", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Cytosine", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "DNA Methylation", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "DNA, Plant", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Genome, Plant", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Meristem", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Polymorphism, Restriction Fragment Length", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Solanum tuberosum", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Time Factors", 
        "type": "DefinedTerm"
      }
    ], 
    "author": [
      {
        "affiliation": {
          "alternateName": "North Dakota State University", 
          "id": "https://www.grid.ac/institutes/grid.261055.5", 
          "name": [
            "United States Department of Agriculture, Agricultural Research Service, Northern Crop Science Laboratory, State University Station, Post Office Box 5677, 58105-5677, Fargo, ND, USA"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Law", 
        "givenName": "R. David", 
        "id": "sg:person.0761211563.73", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0761211563.73"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "North Dakota State University", 
          "id": "https://www.grid.ac/institutes/grid.261055.5", 
          "name": [
            "United States Department of Agriculture, Agricultural Research Service, Northern Crop Science Laboratory, State University Station, Post Office Box 5677, 58105-5677, Fargo, ND, USA"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Suttle", 
        "givenName": "Jeffrey C.", 
        "id": "sg:person.01330647461.61", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01330647461.61"
        ], 
        "type": "Person"
      }
    ], 
    "citation": [
      {
        "id": "https://doi.org/10.1104/pp.90.2.406", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1000176999"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/bf02853997", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1001212220", 
          "https://doi.org/10.1007/bf02853997"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/bf02853997", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1001212220", 
          "https://doi.org/10.1007/bf02853997"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/s0959-437x(00)00061-7", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1003320153"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1023/a:1006457321781", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1003655986", 
          "https://doi.org/10.1023/a:1006457321781"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/s0168-9525(97)01199-2", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1003681939"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1093/nar/22.17.3640", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1004090560"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1111/j.1399-3054.1988.tb00616.x", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1006805454"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1073/pnas.96.11.6107", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1007359638"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1046/j.1365-313x.1996.9050579.x", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1007361909"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1046/j.1365-313x.2000.00805.x", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1009377718"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/s1360-1385(99)01501-0", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1009798859"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1104/pp.39.5.768", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1011613275"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1006/abio.1995.1204", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1013336882"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/0167-4781(90)90100-g", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1013811476"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/0167-4781(90)90100-g", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1013811476"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/13767", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1014815183", 
          "https://doi.org/10.1038/13767"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/13767", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1014815183", 
          "https://doi.org/10.1038/13767"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/bf00234057", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1020215017", 
          "https://doi.org/10.1007/bf00234057"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/bf00234057", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1020215017", 
          "https://doi.org/10.1007/bf00234057"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/5047", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1020518662", 
          "https://doi.org/10.1038/5047"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/5047", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1020518662", 
          "https://doi.org/10.1038/5047"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1046/j.1365-313x.1995.7030471.x", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1020644813"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/s0378-1119(97)00503-9", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1021828413"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/292860a0", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1024461091", 
          "https://doi.org/10.1038/292860a0"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1002/(sici)1097-010x(199809/10)282:1/2<239::aid-jez25>3.0.co;2-n", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1024886379"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/38664", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1026663914", 
          "https://doi.org/10.1038/38664"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/38664", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1026663914", 
          "https://doi.org/10.1038/38664"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1073/pnas.93.22.12406", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1027150465"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/s0065-230x(08)60702-2", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1028310825"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/0092-8674(92)90611-f", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1028643561"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1104/pp.91.2.451", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1030782009"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/s0163-7258(99)00043-1", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1032898159"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/bf00220938", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1036953803", 
          "https://doi.org/10.1007/bf00220938"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1006/bbrc.1999.1187", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1037890372"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/s0168-9452(99)00010-2", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1038093003"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1093/nar/26.10.2255", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1040440102"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1093/nar/23.21.4407", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1042685674"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/s004380050986", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1047572081", 
          "https://doi.org/10.1007/s004380050986"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/s002999900177", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1050528955", 
          "https://doi.org/10.1007/s002999900177"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/s002999900177", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1050528955", 
          "https://doi.org/10.1007/s002999900177"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/s002999900177", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1050528955", 
          "https://doi.org/10.1007/s002999900177"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/0168-9452(93)90061-4", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1050779102"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/bf00015069", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1051345718", 
          "https://doi.org/10.1007/bf00015069"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/bf00015069", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1051345718", 
          "https://doi.org/10.1007/bf00015069"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1104/pp.105.3.891", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1060840398"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1126/science.1059745", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1062444540"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.2144/99262bm11", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1074251812"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.2144/99274st05", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1074535587"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.2144/00294rr02", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1074720229"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1002/j.1460-2075.1994.tb06483.x", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1082692754"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1093/hmg/4.suppl_1.1751", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1082851726"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1079/9780851994475.0211", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1089222894"
        ], 
        "type": "CreativeWork"
      }
    ], 
    "datePublished": "2003-02", 
    "datePublishedReg": "2003-02-01", 
    "description": "The 5-methylcytosine (5mC) content in DNA of tuber meristems isolated from field-grown potatoes (Solanum tuberosum L.) was determined during a 7-month storage period at 3 degrees C for three growing/postharvest seasons. No significant changes in 5mC levels were noted genome-wide or within 5'-CG-3' dinucleotide sequences, 5'-CG-3' islands or 5'-CA(T)G-3' trinucleotide sequences during storage. However, a consistent but transient 50-70% decrease in methylation at both cytosines within 5'-CCGG-3' sequences was detected that peaked 112-194 days after harvest. This result was corroborated by methylation-sensitive amplified fragment length polymorphism analysis of meristem DNA. Similar to tuber meristems undergoing progression through natural dormancy, premature chemical termination of dormancy resulted in rapid, transient 5'-CCGG-3' demethylation in meristem DNA. Minimum methylation levels at this sequence preceded initiation of high levels of de novo DNA synthesis by two days. Cytosine methylation status was also followed in in vitro-generated potato microtubers during 7 months of post-harvest storage. As in DNA from tuber bud meristems, no changes in genome-wide 5mC content or methylation at 5'-CA(T)G-3' or 5'-CG-3' island sequences were noted in microtuber DNA. However, there was a transient 46% drop in methylation at 5'-CG-3' dinucleotides concomitant with minimum levels of 5'-CCGG-3' methylation (30-60% below those in dormant microtubers) 57-98 days after harvest. As microtubers exited dormancy, there were sustained three- and seven-fold increases in RNA and DNA synthesis rates, peaking on or after 98 days of storage, respectively. Together, these data demonstrate that demethylation of 5'-CCGG-3' sequences occurs independently of tuber age during dormancy progression and precedes transcriptional activation of genes leading to cell division and meristem growth in potatoes.", 
    "genre": "research_article", 
    "id": "sg:pub.10.1023/a:1022002304479", 
    "inLanguage": [
      "en"
    ], 
    "isAccessibleForFree": false, 
    "isPartOf": [
      {
        "id": "sg:journal.1101246", 
        "issn": [
          "0167-4412", 
          "1573-5028"
        ], 
        "name": "Plant Molecular Biology", 
        "type": "Periodical"
      }, 
      {
        "issueNumber": "3", 
        "type": "PublicationIssue"
      }, 
      {
        "type": "PublicationVolume", 
        "volumeNumber": "51"
      }
    ], 
    "name": "Transient decreases in methylation at 5\u2032-CCGG-3\u2032 sequences in potato (Solanum tuberosum L.) meristem DNA during progression of tubers through dormancy precede the resumption of sprout growth", 
    "pagination": "437-447", 
    "productId": [
      {
        "name": "readcube_id", 
        "type": "PropertyValue", 
        "value": [
          "5499811a62e9f0493449c5b651d8e817c6a33f685f9ddf94b4c2f38f9dda90dc"
        ]
      }, 
      {
        "name": "pubmed_id", 
        "type": "PropertyValue", 
        "value": [
          "12602873"
        ]
      }, 
      {
        "name": "nlm_unique_id", 
        "type": "PropertyValue", 
        "value": [
          "9106343"
        ]
      }, 
      {
        "name": "doi", 
        "type": "PropertyValue", 
        "value": [
          "10.1023/a:1022002304479"
        ]
      }, 
      {
        "name": "dimensions_id", 
        "type": "PropertyValue", 
        "value": [
          "pub.1017755717"
        ]
      }
    ], 
    "sameAs": [
      "https://doi.org/10.1023/a:1022002304479", 
      "https://app.dimensions.ai/details/publication/pub.1017755717"
    ], 
    "sdDataset": "articles", 
    "sdDatePublished": "2019-04-10T21:35", 
    "sdLicense": "https://scigraph.springernature.com/explorer/license/", 
    "sdPublisher": {
      "name": "Springer Nature - SN SciGraph project", 
      "type": "Organization"
    }, 
    "sdSource": "s3://com-uberresearch-data-dimensions-target-20181106-alternative/cleanup/v134/2549eaecd7973599484d7c17b260dba0a4ecb94b/merge/v9/a6c9fde33151104705d4d7ff012ea9563521a3ce/jats-lookup/v90/0000000001_0000000264/records_8687_00000504.jsonl", 
    "type": "ScholarlyArticle", 
    "url": "http://link.springer.com/10.1023%2FA%3A1022002304479"
  }
]
 

Download the RDF metadata as:  json-ld nt turtle xml License info

HOW TO GET THIS DATA PROGRAMMATICALLY:

JSON-LD is a popular format for linked data which is fully compatible with JSON.

curl -H 'Accept: application/ld+json' 'https://scigraph.springernature.com/pub.10.1023/a:1022002304479'

N-Triples is a line-based linked data format ideal for batch operations.

curl -H 'Accept: application/n-triples' 'https://scigraph.springernature.com/pub.10.1023/a:1022002304479'

Turtle is a human-readable linked data format.

curl -H 'Accept: text/turtle' 'https://scigraph.springernature.com/pub.10.1023/a:1022002304479'

RDF/XML is a standard XML format for linked data.

curl -H 'Accept: application/rdf+xml' 'https://scigraph.springernature.com/pub.10.1023/a:1022002304479'


 

This table displays all metadata directly associated to this object as RDF triples.

263 TRIPLES      21 PREDICATES      84 URIs      32 LITERALS      20 BLANK NODES

Subject Predicate Object
1 sg:pub.10.1023/a:1022002304479 schema:about N041207998d5a42f0a0595552e294f30a
2 N0cf11ade56ef4e05a7ea77c4dbe01b60
3 N3674ca9540054bacb1485bd589e37da3
4 N3de4e161dd404382ab24c4755a706230
5 N60e9cb554f7b4c95bd387c7eb614a2b3
6 N6ada28e00ccc40e39429b03691a18d99
7 Na101543355d840c6b6213ad58013e1bc
8 Nac0140cda6774f299b22abdefa31d577
9 Nd4d2c15ffd32496d8282dad8e32c4f75
10 Nf7d2d6531e6d4eaa9e4d9f7501964d90
11 Nf83d171420d94931b87fbcc4895fc783
12 anzsrc-for:06
13 anzsrc-for:0604
14 schema:author N8856240bf9984fea82f65e62d55ba5b7
15 schema:citation sg:pub.10.1007/bf00015069
16 sg:pub.10.1007/bf00220938
17 sg:pub.10.1007/bf00234057
18 sg:pub.10.1007/bf02853997
19 sg:pub.10.1007/s002999900177
20 sg:pub.10.1007/s004380050986
21 sg:pub.10.1023/a:1006457321781
22 sg:pub.10.1038/13767
23 sg:pub.10.1038/292860a0
24 sg:pub.10.1038/38664
25 sg:pub.10.1038/5047
26 https://doi.org/10.1002/(sici)1097-010x(199809/10)282:1/2<239::aid-jez25>3.0.co;2-n
27 https://doi.org/10.1002/j.1460-2075.1994.tb06483.x
28 https://doi.org/10.1006/abio.1995.1204
29 https://doi.org/10.1006/bbrc.1999.1187
30 https://doi.org/10.1016/0092-8674(92)90611-f
31 https://doi.org/10.1016/0167-4781(90)90100-g
32 https://doi.org/10.1016/0168-9452(93)90061-4
33 https://doi.org/10.1016/s0065-230x(08)60702-2
34 https://doi.org/10.1016/s0163-7258(99)00043-1
35 https://doi.org/10.1016/s0168-9452(99)00010-2
36 https://doi.org/10.1016/s0168-9525(97)01199-2
37 https://doi.org/10.1016/s0378-1119(97)00503-9
38 https://doi.org/10.1016/s0959-437x(00)00061-7
39 https://doi.org/10.1016/s1360-1385(99)01501-0
40 https://doi.org/10.1046/j.1365-313x.1995.7030471.x
41 https://doi.org/10.1046/j.1365-313x.1996.9050579.x
42 https://doi.org/10.1046/j.1365-313x.2000.00805.x
43 https://doi.org/10.1073/pnas.93.22.12406
44 https://doi.org/10.1073/pnas.96.11.6107
45 https://doi.org/10.1079/9780851994475.0211
46 https://doi.org/10.1093/hmg/4.suppl_1.1751
47 https://doi.org/10.1093/nar/22.17.3640
48 https://doi.org/10.1093/nar/23.21.4407
49 https://doi.org/10.1093/nar/26.10.2255
50 https://doi.org/10.1104/pp.105.3.891
51 https://doi.org/10.1104/pp.39.5.768
52 https://doi.org/10.1104/pp.90.2.406
53 https://doi.org/10.1104/pp.91.2.451
54 https://doi.org/10.1111/j.1399-3054.1988.tb00616.x
55 https://doi.org/10.1126/science.1059745
56 https://doi.org/10.2144/00294rr02
57 https://doi.org/10.2144/99262bm11
58 https://doi.org/10.2144/99274st05
59 schema:datePublished 2003-02
60 schema:datePublishedReg 2003-02-01
61 schema:description The 5-methylcytosine (5mC) content in DNA of tuber meristems isolated from field-grown potatoes (Solanum tuberosum L.) was determined during a 7-month storage period at 3 degrees C for three growing/postharvest seasons. No significant changes in 5mC levels were noted genome-wide or within 5'-CG-3' dinucleotide sequences, 5'-CG-3' islands or 5'-CA(T)G-3' trinucleotide sequences during storage. However, a consistent but transient 50-70% decrease in methylation at both cytosines within 5'-CCGG-3' sequences was detected that peaked 112-194 days after harvest. This result was corroborated by methylation-sensitive amplified fragment length polymorphism analysis of meristem DNA. Similar to tuber meristems undergoing progression through natural dormancy, premature chemical termination of dormancy resulted in rapid, transient 5'-CCGG-3' demethylation in meristem DNA. Minimum methylation levels at this sequence preceded initiation of high levels of de novo DNA synthesis by two days. Cytosine methylation status was also followed in in vitro-generated potato microtubers during 7 months of post-harvest storage. As in DNA from tuber bud meristems, no changes in genome-wide 5mC content or methylation at 5'-CA(T)G-3' or 5'-CG-3' island sequences were noted in microtuber DNA. However, there was a transient 46% drop in methylation at 5'-CG-3' dinucleotides concomitant with minimum levels of 5'-CCGG-3' methylation (30-60% below those in dormant microtubers) 57-98 days after harvest. As microtubers exited dormancy, there were sustained three- and seven-fold increases in RNA and DNA synthesis rates, peaking on or after 98 days of storage, respectively. Together, these data demonstrate that demethylation of 5'-CCGG-3' sequences occurs independently of tuber age during dormancy progression and precedes transcriptional activation of genes leading to cell division and meristem growth in potatoes.
62 schema:genre research_article
63 schema:inLanguage en
64 schema:isAccessibleForFree false
65 schema:isPartOf N7de93660d4074ba8898801d1e78fab0f
66 Nf8434f96eaf5470bb56abf6aee13358e
67 sg:journal.1101246
68 schema:name Transient decreases in methylation at 5′-CCGG-3′ sequences in potato (Solanum tuberosum L.) meristem DNA during progression of tubers through dormancy precede the resumption of sprout growth
69 schema:pagination 437-447
70 schema:productId N063d1b8608a94464ae5873add45af3d3
71 N1c3a277582a7481c97e6380a313d8cd8
72 N368e0b1925cf4860adec2af11d95d3df
73 N547a3524710e452b9dd523c950f4ffc8
74 N5baac9f7f3fe4a50b06d4273517e1a34
75 schema:sameAs https://app.dimensions.ai/details/publication/pub.1017755717
76 https://doi.org/10.1023/a:1022002304479
77 schema:sdDatePublished 2019-04-10T21:35
78 schema:sdLicense https://scigraph.springernature.com/explorer/license/
79 schema:sdPublisher Nacd539d96dea4390bb784c7ac1177687
80 schema:url http://link.springer.com/10.1023%2FA%3A1022002304479
81 sgo:license sg:explorer/license/
82 sgo:sdDataset articles
83 rdf:type schema:ScholarlyArticle
84 N041207998d5a42f0a0595552e294f30a schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
85 schema:name Solanum tuberosum
86 rdf:type schema:DefinedTerm
87 N063d1b8608a94464ae5873add45af3d3 schema:name nlm_unique_id
88 schema:value 9106343
89 rdf:type schema:PropertyValue
90 N0cf11ade56ef4e05a7ea77c4dbe01b60 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
91 schema:name Time Factors
92 rdf:type schema:DefinedTerm
93 N1c3a277582a7481c97e6380a313d8cd8 schema:name readcube_id
94 schema:value 5499811a62e9f0493449c5b651d8e817c6a33f685f9ddf94b4c2f38f9dda90dc
95 rdf:type schema:PropertyValue
96 N3674ca9540054bacb1485bd589e37da3 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
97 schema:name Cytosine
98 rdf:type schema:DefinedTerm
99 N368e0b1925cf4860adec2af11d95d3df schema:name dimensions_id
100 schema:value pub.1017755717
101 rdf:type schema:PropertyValue
102 N3de4e161dd404382ab24c4755a706230 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
103 schema:name Polymorphism, Restriction Fragment Length
104 rdf:type schema:DefinedTerm
105 N547a3524710e452b9dd523c950f4ffc8 schema:name doi
106 schema:value 10.1023/a:1022002304479
107 rdf:type schema:PropertyValue
108 N5baac9f7f3fe4a50b06d4273517e1a34 schema:name pubmed_id
109 schema:value 12602873
110 rdf:type schema:PropertyValue
111 N60e9cb554f7b4c95bd387c7eb614a2b3 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
112 schema:name DNA, Plant
113 rdf:type schema:DefinedTerm
114 N6ada28e00ccc40e39429b03691a18d99 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
115 schema:name DNA Methylation
116 rdf:type schema:DefinedTerm
117 N7de93660d4074ba8898801d1e78fab0f schema:issueNumber 3
118 rdf:type schema:PublicationIssue
119 N87becb7e0e51417aaf61c86ea3ae7776 rdf:first sg:person.01330647461.61
120 rdf:rest rdf:nil
121 N8856240bf9984fea82f65e62d55ba5b7 rdf:first sg:person.0761211563.73
122 rdf:rest N87becb7e0e51417aaf61c86ea3ae7776
123 Na101543355d840c6b6213ad58013e1bc schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
124 schema:name Binding Sites
125 rdf:type schema:DefinedTerm
126 Nac0140cda6774f299b22abdefa31d577 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
127 schema:name Genome, Plant
128 rdf:type schema:DefinedTerm
129 Nacd539d96dea4390bb784c7ac1177687 schema:name Springer Nature - SN SciGraph project
130 rdf:type schema:Organization
131 Nd4d2c15ffd32496d8282dad8e32c4f75 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
132 schema:name Base Sequence
133 rdf:type schema:DefinedTerm
134 Nf7d2d6531e6d4eaa9e4d9f7501964d90 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
135 schema:name 5-Methylcytosine
136 rdf:type schema:DefinedTerm
137 Nf83d171420d94931b87fbcc4895fc783 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
138 schema:name Meristem
139 rdf:type schema:DefinedTerm
140 Nf8434f96eaf5470bb56abf6aee13358e schema:volumeNumber 51
141 rdf:type schema:PublicationVolume
142 anzsrc-for:06 schema:inDefinedTermSet anzsrc-for:
143 schema:name Biological Sciences
144 rdf:type schema:DefinedTerm
145 anzsrc-for:0604 schema:inDefinedTermSet anzsrc-for:
146 schema:name Genetics
147 rdf:type schema:DefinedTerm
148 sg:journal.1101246 schema:issn 0167-4412
149 1573-5028
150 schema:name Plant Molecular Biology
151 rdf:type schema:Periodical
152 sg:person.01330647461.61 schema:affiliation https://www.grid.ac/institutes/grid.261055.5
153 schema:familyName Suttle
154 schema:givenName Jeffrey C.
155 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01330647461.61
156 rdf:type schema:Person
157 sg:person.0761211563.73 schema:affiliation https://www.grid.ac/institutes/grid.261055.5
158 schema:familyName Law
159 schema:givenName R. David
160 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0761211563.73
161 rdf:type schema:Person
162 sg:pub.10.1007/bf00015069 schema:sameAs https://app.dimensions.ai/details/publication/pub.1051345718
163 https://doi.org/10.1007/bf00015069
164 rdf:type schema:CreativeWork
165 sg:pub.10.1007/bf00220938 schema:sameAs https://app.dimensions.ai/details/publication/pub.1036953803
166 https://doi.org/10.1007/bf00220938
167 rdf:type schema:CreativeWork
168 sg:pub.10.1007/bf00234057 schema:sameAs https://app.dimensions.ai/details/publication/pub.1020215017
169 https://doi.org/10.1007/bf00234057
170 rdf:type schema:CreativeWork
171 sg:pub.10.1007/bf02853997 schema:sameAs https://app.dimensions.ai/details/publication/pub.1001212220
172 https://doi.org/10.1007/bf02853997
173 rdf:type schema:CreativeWork
174 sg:pub.10.1007/s002999900177 schema:sameAs https://app.dimensions.ai/details/publication/pub.1050528955
175 https://doi.org/10.1007/s002999900177
176 rdf:type schema:CreativeWork
177 sg:pub.10.1007/s004380050986 schema:sameAs https://app.dimensions.ai/details/publication/pub.1047572081
178 https://doi.org/10.1007/s004380050986
179 rdf:type schema:CreativeWork
180 sg:pub.10.1023/a:1006457321781 schema:sameAs https://app.dimensions.ai/details/publication/pub.1003655986
181 https://doi.org/10.1023/a:1006457321781
182 rdf:type schema:CreativeWork
183 sg:pub.10.1038/13767 schema:sameAs https://app.dimensions.ai/details/publication/pub.1014815183
184 https://doi.org/10.1038/13767
185 rdf:type schema:CreativeWork
186 sg:pub.10.1038/292860a0 schema:sameAs https://app.dimensions.ai/details/publication/pub.1024461091
187 https://doi.org/10.1038/292860a0
188 rdf:type schema:CreativeWork
189 sg:pub.10.1038/38664 schema:sameAs https://app.dimensions.ai/details/publication/pub.1026663914
190 https://doi.org/10.1038/38664
191 rdf:type schema:CreativeWork
192 sg:pub.10.1038/5047 schema:sameAs https://app.dimensions.ai/details/publication/pub.1020518662
193 https://doi.org/10.1038/5047
194 rdf:type schema:CreativeWork
195 https://doi.org/10.1002/(sici)1097-010x(199809/10)282:1/2<239::aid-jez25>3.0.co;2-n schema:sameAs https://app.dimensions.ai/details/publication/pub.1024886379
196 rdf:type schema:CreativeWork
197 https://doi.org/10.1002/j.1460-2075.1994.tb06483.x schema:sameAs https://app.dimensions.ai/details/publication/pub.1082692754
198 rdf:type schema:CreativeWork
199 https://doi.org/10.1006/abio.1995.1204 schema:sameAs https://app.dimensions.ai/details/publication/pub.1013336882
200 rdf:type schema:CreativeWork
201 https://doi.org/10.1006/bbrc.1999.1187 schema:sameAs https://app.dimensions.ai/details/publication/pub.1037890372
202 rdf:type schema:CreativeWork
203 https://doi.org/10.1016/0092-8674(92)90611-f schema:sameAs https://app.dimensions.ai/details/publication/pub.1028643561
204 rdf:type schema:CreativeWork
205 https://doi.org/10.1016/0167-4781(90)90100-g schema:sameAs https://app.dimensions.ai/details/publication/pub.1013811476
206 rdf:type schema:CreativeWork
207 https://doi.org/10.1016/0168-9452(93)90061-4 schema:sameAs https://app.dimensions.ai/details/publication/pub.1050779102
208 rdf:type schema:CreativeWork
209 https://doi.org/10.1016/s0065-230x(08)60702-2 schema:sameAs https://app.dimensions.ai/details/publication/pub.1028310825
210 rdf:type schema:CreativeWork
211 https://doi.org/10.1016/s0163-7258(99)00043-1 schema:sameAs https://app.dimensions.ai/details/publication/pub.1032898159
212 rdf:type schema:CreativeWork
213 https://doi.org/10.1016/s0168-9452(99)00010-2 schema:sameAs https://app.dimensions.ai/details/publication/pub.1038093003
214 rdf:type schema:CreativeWork
215 https://doi.org/10.1016/s0168-9525(97)01199-2 schema:sameAs https://app.dimensions.ai/details/publication/pub.1003681939
216 rdf:type schema:CreativeWork
217 https://doi.org/10.1016/s0378-1119(97)00503-9 schema:sameAs https://app.dimensions.ai/details/publication/pub.1021828413
218 rdf:type schema:CreativeWork
219 https://doi.org/10.1016/s0959-437x(00)00061-7 schema:sameAs https://app.dimensions.ai/details/publication/pub.1003320153
220 rdf:type schema:CreativeWork
221 https://doi.org/10.1016/s1360-1385(99)01501-0 schema:sameAs https://app.dimensions.ai/details/publication/pub.1009798859
222 rdf:type schema:CreativeWork
223 https://doi.org/10.1046/j.1365-313x.1995.7030471.x schema:sameAs https://app.dimensions.ai/details/publication/pub.1020644813
224 rdf:type schema:CreativeWork
225 https://doi.org/10.1046/j.1365-313x.1996.9050579.x schema:sameAs https://app.dimensions.ai/details/publication/pub.1007361909
226 rdf:type schema:CreativeWork
227 https://doi.org/10.1046/j.1365-313x.2000.00805.x schema:sameAs https://app.dimensions.ai/details/publication/pub.1009377718
228 rdf:type schema:CreativeWork
229 https://doi.org/10.1073/pnas.93.22.12406 schema:sameAs https://app.dimensions.ai/details/publication/pub.1027150465
230 rdf:type schema:CreativeWork
231 https://doi.org/10.1073/pnas.96.11.6107 schema:sameAs https://app.dimensions.ai/details/publication/pub.1007359638
232 rdf:type schema:CreativeWork
233 https://doi.org/10.1079/9780851994475.0211 schema:sameAs https://app.dimensions.ai/details/publication/pub.1089222894
234 rdf:type schema:CreativeWork
235 https://doi.org/10.1093/hmg/4.suppl_1.1751 schema:sameAs https://app.dimensions.ai/details/publication/pub.1082851726
236 rdf:type schema:CreativeWork
237 https://doi.org/10.1093/nar/22.17.3640 schema:sameAs https://app.dimensions.ai/details/publication/pub.1004090560
238 rdf:type schema:CreativeWork
239 https://doi.org/10.1093/nar/23.21.4407 schema:sameAs https://app.dimensions.ai/details/publication/pub.1042685674
240 rdf:type schema:CreativeWork
241 https://doi.org/10.1093/nar/26.10.2255 schema:sameAs https://app.dimensions.ai/details/publication/pub.1040440102
242 rdf:type schema:CreativeWork
243 https://doi.org/10.1104/pp.105.3.891 schema:sameAs https://app.dimensions.ai/details/publication/pub.1060840398
244 rdf:type schema:CreativeWork
245 https://doi.org/10.1104/pp.39.5.768 schema:sameAs https://app.dimensions.ai/details/publication/pub.1011613275
246 rdf:type schema:CreativeWork
247 https://doi.org/10.1104/pp.90.2.406 schema:sameAs https://app.dimensions.ai/details/publication/pub.1000176999
248 rdf:type schema:CreativeWork
249 https://doi.org/10.1104/pp.91.2.451 schema:sameAs https://app.dimensions.ai/details/publication/pub.1030782009
250 rdf:type schema:CreativeWork
251 https://doi.org/10.1111/j.1399-3054.1988.tb00616.x schema:sameAs https://app.dimensions.ai/details/publication/pub.1006805454
252 rdf:type schema:CreativeWork
253 https://doi.org/10.1126/science.1059745 schema:sameAs https://app.dimensions.ai/details/publication/pub.1062444540
254 rdf:type schema:CreativeWork
255 https://doi.org/10.2144/00294rr02 schema:sameAs https://app.dimensions.ai/details/publication/pub.1074720229
256 rdf:type schema:CreativeWork
257 https://doi.org/10.2144/99262bm11 schema:sameAs https://app.dimensions.ai/details/publication/pub.1074251812
258 rdf:type schema:CreativeWork
259 https://doi.org/10.2144/99274st05 schema:sameAs https://app.dimensions.ai/details/publication/pub.1074535587
260 rdf:type schema:CreativeWork
261 https://www.grid.ac/institutes/grid.261055.5 schema:alternateName North Dakota State University
262 schema:name United States Department of Agriculture, Agricultural Research Service, Northern Crop Science Laboratory, State University Station, Post Office Box 5677, 58105-5677, Fargo, ND, USA
263 rdf:type schema:Organization
 




Preview window. Press ESC to close (or click here)


...